Have a personal or library account? Click to login
The use of pre-rooted in-vitro grapevine shoot segments for elimination of Arabis mosaic virus with chemotherapy Cover

The use of pre-rooted in-vitro grapevine shoot segments for elimination of Arabis mosaic virus with chemotherapy

Open Access
|Jan 2026

References

  1. Chauhan, P., Singla, K., Rajbhar, M., Singh, A., Das, N., Kumar, K. 2019: A systematic review of conventional and advanced approaches for the control of plant viruses. Journal of Applied Biology and Biotechnology 7(4): 89-98.
  2. Data Visualization with R and Ggplot2 | the R Graph Gallery Available online: https://r-graph-gallery.com/ggplot2-package.html (28.02.2023)
  3. Digiaro, M., Elbeaino, T., Martelli, G.P. 2017: Grapevine fanleaf virus and other old world nepoviruses. In: Meng, B., Martelli, G., Golino, D., Fuchs, M. (eds) Grapevine Viruses: Molecular Biology, Diagnostics and Management. - Springer, Cham, 2017, S. 47-82.
  4. Eichmeier, A., Kominkova, M., Pecenka, J., Kominek, P. 2019: High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy. Journal of Virological Methods 267: 66-70.
  5. Elkin, L.A., Kay, M., Higgins, J.J., Wobbrock, J.O. 2021: An aligned rank transform procedure for multifactor contrast tests. UIST 2021 - Proceedings of the 34th Annual ACM Symposium on User Interface Software and Technology, S. 754–768. doi:10.1145/3472749.3474784.
  6. Galbacs, Z.S., Molnar, S., Halasz, G., Kozma, P., Hoffmann, S., Kovács, L., Veres, A., Galli, Z.S., Szőke, A., Heszky, L., Kiss, E. 2009: Identification of grapevine cultivars using microsatellite-based DNA barcodes. VITIS-Journal of Grapevine Research 48(1): 17-24.
  7. Gambino, G., Gribaudo, I. 2006: Simultaneous detection of nine grapevine viruses by multiplex reverse transcription-polymerase chain reaction with coamplification of a plant RNA as internal control. Phytopathology 96(11): 1223-1229.
  8. Gao, F., Lin, W., Shen, J., Liao, F. 2016: Genetic diversity and molecular evolution of arabis mosaic virus based on the CP gene sequence. Archives of Virology 161(4): 1047-1051.
  9. Guţă, I.C., Buciumeanu, E.C. 2011: Grapevine chemotherapy for elimination of multiple virus infection. Romanian Biotechnological Letters 16(5): 6535-6539.
  10. Hu, G., Dong, Y., Zhang, Z., Fan, X., Ren, F., Li, Z., Zhang, S. 2018: Elimination of Grapevine rupestris stem pitting-associated virus from Vitis vinifera ‘Kyoho’by an antiviral agent combined with shoot tip culture. Scientia Horticulturae 229: 99-106.
  11. Hu, G., Dong, Y., Zhang, Z., Fan, X., Ren, F. 2022: Inefficiency of ribavirin to eliminate apple scar skin viroid from apple plants. Plant Cell, Tissue and Organ Culture (PCTOC) 151(1): 189-197.
  12. Komínek, P., Komínková, M., Jandová, B. 2016: Effect of repeated Ribavirin treatment on grapevine viruses. Acta Virologica 60(4): 400-403.
  13. Komorowska, B., Hasiów-Jaroszewska, B., Budzyńska, D. 2021: Genetic variability and molecular evolution of arabis mosaic virus based on the coat protein gene sequence. Plant Pathology 70(9): 2197-2206.
  14. Magyar-Tábori, K., Mendler-Drienyovszki, N., Hanász, A., Zsombik, L., Dobránszki, J. 2021: phytotoxicity and other adverse effects on the in-vitro shoot cultures caused by virus elimination treatments: Reasons and Solutions. Plants 10(4): 670.
  15. Modarresi Chahardehi, A., Rakhshandehroo, F., Mozafari, J., Mousavi, L. 2016: Efficiency of a chemo-thermotherapy technique for eliminating Arabis mosaic virus (ArMV) and Prunus necrotic ringspot virus (PNRSV) from in vitro rose plantlets. Journal of Crop Protection 5(4): 497-506.
  16. Olah, R., Turcsan, M., Olah, K., Farkas, E., Deak, T., Jahnke, G., Sardy, D.A.N. 2022: Somatic Embryogenesis: A Tool for Fast and Reliable Virus and Viroid Elimination for Grapevine and other Plant Species. Horticulturae 8(6): 508.
  17. Panattoni, A., Luvisi, A., Triolo, E. 2013: Review. Elimination of Viruses in Plants: Twenty Years of Progress. Span. J. Agric. Res. 11: 173–188. https://doi.org/10.5424/sjar/2013111-3201.
  18. R Core Team R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. Available online: http://www.r-project.org/.
  19. Shatnawi, M., Anfoka, G., Shibli, R., Al-Mazra'awi, M., Shahrour, W., Arebiat, A. 2011: Clonal propagation and cryogenic storage of virus-free grapevine (Vitis vinifera L.) via meristem culture. Turkish Journal of Agriculture and Forestry 35(2): 173-184.
  20. Skiada, F.G., Maliogka, V.I., Katis, N.I., Eleftheriou, E.P. 2013: Elimination of Grapevine rupestris stem pitting-associated virus (GRSPaV) from two Vitis vinifera cultivars by in vitro chemotherapy. European journal of plant pathology 135: 407-414.
  21. Szegedi, E., Deák, T., Turcsán, M., Szénási, M., Bordé, Á., Oláh, R. 2018: Evaluation of intron containing potential reference gene-specific primers to validate grapevine nucleic acid samples prepared for conventional PCR and RT-PCR. Vitis 57(2): 69-73.
  22. Xu, Q., Wen, X., Deng, X. 2004: A simple protocol for isolating genomic DNA from chestnut rose (Rosa roxburghii Tratt) for RFLP and PCR analyses. Plant Molecular Biology Reporter 22: 301-302.
  23. Wang, M.R., Cui, Z.H., Li, J.W., Hao, X.Y., Zhao, L., Wang, Q.C. 2018: In vitro thermotherapy-based methods for plant virus eradication. Plant methods 14(1): 1-18.
  24. Weiland, C.M., Cantos, M., Troncoso, A., Perez-Camacho, F. 2004: Regeneration of virus-free plants by in vitro chemotherapy of GFLV (Grapevine fanleaf virus) infected explants of Vitis vinifera L. Cv'Zalema'. Acta Hortic. 652: 463-466.
  25. Wobbrock, J.O., Findlater, L., Gergle, D., Higgins, J.J. 2011: The Aligned Rank Transform for Nonparametric Factorial Analyses Using Only ANOVA Procedures. Conference on Human Factors in Computing Systems – Proceedings. S. 143–146. doi:10.1145/1978942.1978963.
DOI: https://doi.org/10.2478/mittklbg-2023-0011 | Journal eISSN: 3061-063X | Journal ISSN: 3061-0621
Language: English
Page range: 168 - 175
Submitted on: Apr 26, 2023
|
Published on: Jan 9, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 Krisztina Oláh, Mihály Turcsán, Gizella Jahnke, Tamás Deák, Róbert Oláh, Diána Ágnes Nyitrainé Sárdy, published by High School and Federal Office of Viticulture and Pomology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.