Skip to main content
Have a personal or library account? Click to login

               Pratylenchus pseudocoffeae Mizukubo, 1992 infecting dahlia (Dahlia hybrida) in the United States expands its known host and geographic ranges Cover

Pratylenchus pseudocoffeae Mizukubo, 1992 infecting dahlia (Dahlia hybrida) in the United States expands its known host and geographic ranges

Open Access
|Jul 2026

References

  1. Burnett, S. E., Peterson, B. J., Oliveira, I. and Bowers, T. 2023. Comparison of dahlia cultivars for cut flower production in the Northeastern United States. HortTechnology 33(5):419–424.
  2. Castillo, P. and Vovlas, N. 2007. Pratylenchus, Nematoda, Pratylenchidae: Diagnosis, biology, pathogenicity, and management. Nematology Monographs & Perspectives 6:1–530.
  3. Chenna, R., Sugawara, H., Koike, T., Lopez, Z., R., Gibson, T. J., Higgins, D. G. and Thompson, J. D. 2003. Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Research 31:3497–3500. 10.1093/nar/gkg500
  4. Ciobanu, E. C., Bădulescu, L., Badea, M. L. and Toma, F. 2021. Studies and research on the species and varieties of Dahlia in cultivation. Scientific Papers. Series B. Horticulture 65(1):683–689.
  5. Coyne, D. L., Adewuyi, O. and Mbiru, E. 2014. Protocol for in vitro culturing of lesion nematodes: Radopholus similis and Pratylenchus spp. on carrot discs. Ibadan, Nigeria: International Institute of Tropical Agriculture (IITA). Available online at https://biblio.iita.org/documents/U14ManCoyneProtocolNothomNodev.pdf-6b2ee7a4acb3c78c3fadfef69b39776a.pdf
  6. De Ley, P., De Ley, I. T., Morris, K., Abebe, E., Mundo-Ocampo, M., Yoder, M., Heras, J., Waumann, D., Rocha-Olivares, A., Burr, A. H. J., Baldwin, J. G. and Thomas, W. K. 2005. An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 360(1462):1945–1958. https://doi.org/10.1098/rstb.2005.1726
  7. Deimi, A. M., De Luca, F. and Vovlas, N. 2009. Characterization and parasitic habits of a root-lesion nematode from chrysanthemum in Iran and its relationship to Pratylenchus pseudocoffeae. Nematology 11(5):757–768.
  8. Fox, J. and Weisberg, S. 2019. An R companion to applied regression, 3rd edition. Thousand Oaks, CA: Sage. https://www.john-fox.ca/Companion/
  9. Frey, J., Frey, B., Frei, D., Gueuning, M., Blaser, S., Bühlmann, A. 2022. Workflow for the nucleic acid-based identification of insects using whole genome amplification and nanopore sequencing - kawa v.1. Protocols.io. Available at https://www.protocols.io/view/workflow-for-the-nucleic-acid-based-identification-261ge41kdv47/v1
  10. Handoo, Z. A., Yan, G., Kantor, M. R., Huang, D., Chowdhury, I. A., Plaisance, A., Bauchan, G. R. and Mowery, J. D. 2021. Morphological and molecular characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a new root-lesion nematode species on soybean in North Dakota, USA. Plants 10(1):168.
  11. Hothorn, T., Bretz, F. and Westfall, P. 2008. Simultaneous inference in general parametric models. Biometrical Journal 50(3):346–363.
  12. Howlett, B. J., Brownlee, A. G., Guest, D. I., Adcock, G. J. and McFadden, G. I. 1992. The 5S ribosomal RNA gene is linked to large and small subunit ribosomal RNA genes in the oomycetes, Phytopthora vignae, P. cinnamomi, P. megasperma f. sp. glycinae and Saprolegnia ferax. Current Genetics 22:455–461. 10.1007/BF00326410.
  13. Inserra, R. N., Duncan, L. W., Dunn, D., Kaplan, D. T. and Porazinska, D. L. 1998. Pratylenchus pseudocoffeae from Florida and its relationship with P. gutierrezi and P. coffeae. Nematologica 44(6):683–712. https://doi.org/10.1163/005725998X00087
  14. Joyce, S. A., Reid, A., Driver, F., Curran, J. 1994. Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes. pp. 178–187 in A. M. Burnell, R.-U. Ehlers and J. P. Masson, eds. COST 812 Biotechnology: Genetics of Entomopathogenic Nematode-Bacterium Complexes; Proceedings of Symposium & Workshop; St. Patrick’s College, Maynooth, County Kildare, Ireland. Luxembourg: European Commission. DG XII.
  15. Kantor, M., Handoo, Z., Kantor, C. and Carta, L. 2022. Top ten most important US-regulated and emerging plant-parasitic nematodes. Horticulturae 8(3):208. https://doi.org/10.3390/horticulturae8030208
  16. Kawasaki, E. S. 1990. Sample preparation from blood, cells and other fluids. pp. 146–152 in M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, eds. PCR protocols. London, UK: Academic Press.
  17. Kim, D., Chun, J.-Y. and Lee, K.-Y. 2016. Morphometric and molecular characterization of populations of Pratylenchus kumamotoensis and P. pseudocoffeae (Nematoda, Pratylenchidae) newly recorded in Korea. ZooKeys 600:1–5. https://doi.org/10.3897/zookeys.600.8508
  18. Kushida, A. and Kondo, N. 2015. A simple method for the detection and discrimination of Pratylenchus and Meloidogyne species in nematode communities. Nematological Research. 45:101–114.
  19. Mizukubo, T. 1992. Pratylenchus pseudocoffeae n. sp. (Nematoda: Pratylenchidae) from composite plants in Japan. Applied Entomology and Zoology 27(3):437–444. https://doi.org/10.1303/aez.27.437
  20. Nicholas, K. B., Nicholas Jr, H. B. and DeerWeld II, D. W. 1997. GeneDoc: Analysis and visualization of genetic variation. EMBNEW News 867:4–14.
  21. Posada, D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25:1253–1256. 10. 1093/molbev/msn083
  22. R Core Team. 2023. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing.
  23. Ronquist, F. and Huelsenbeck, J. P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574.
  24. Santana, L. S., Villanueva-Carvajal, A., Morales-Rosales, E. J., Laguna-Cerda, A. and Dominguez-Lopez, A. 2016. Evaluation of inulin extracted from Mexican wild dahlias (Dahlia coccinea Cav.). Phyton 85:63–70.
  25. Subbotin, S., Waeyenberge, L. and Moens, M. 2000. Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNA-RFLP. Nematology 2(2):153–164.
  26. Subbotin, S. A. 2021. Phylogenetic analysis of DNA sequence data. pp. 265–282 in R. N. Perry, D. J. Hunt and S. A. Subbotin, eds. Techniques for work with plant and soil nematodes, Wallingford, UK: CAB International.
  27. Swofford, D. L. 2003. PAUP*: phylogenetic analysis using parsimony (*and other methods), Version 4.0b 10. Sunder land, MA, USA: Sinauer Associates.
  28. Uesugi, K., Iwahori, H. and Tateishi, Y. 2012. Pathogenicity of Pratylenchus pseudocoffeae in chrysanthemum and its reproduction on 23 crops. Nematological Research (Japanese Journal of Nematology) 42(2):31–34.
  29. USDA-NASS. 2024. 2023 Floriculture crops highlights. Washington, DC: United States Department of Agriculture, National Agricultural Statistics Service. Available at https://www.nass.usda.gov/Publications/Highlights/2024/2023-floriculture-highlights.pdf
  30. Viaene, N., Hallmann, J. and Molendijk, L. P. G. 2021. Methods for nematode extraction. pp. 29–59 in R. N. Perry, D. J. Hunt and S. A. Subbotin, eds. Techniques for work with plant and soil nematodes. Oxfordshire, UK: CAB International.
  31. Wickham, H., Averick, M, Bryan, J., Chang, W., McGowan, L. D., François, R., Grolemund, G., Hayes, A., Henry, L., Hester, J., Kuhn, M., Pedersen, T. L., Miller, E., Bache, S. M., Müller, K., Ooms, J., Robinson, D., Seidel, D. P, Spinu, V., Takahashi, K., Vaughan, D., Wilke, C., Woo, K. and Yutani, H. 2019. Welcome to the tidyverse. Journal of Open Source Software 4(43):1686.
  32. Williams, B. D., Schrank, B., Huynh, C., Shownkeen, R., Waterston, R. H. 1992. A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131(3):609–624. 10.1093/genetics/131.3.609
DOI: https://doi.org/10.2478/jofnem-2026-0019 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 246 - 257
Submitted on: May 1, 2026
Accepted on: Jun 17, 2026
Published on: Jul 30, 2026
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2026 Erika Consoli, Mesfin Bogale, Sandip Mondal, Timothy I. Ralston, Alemayehu Habteweld, Zafar Handoo, Emily Wolf, Paulo Vieira, Francesca Peduto Hand, Sara R. May, Horacio D. Lopez-Nicora, Mihail Kantor, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.