Journal of Nematology Feed

Host status of sweetpotato cultivars and germplasm to Rotylenchulus reniformis

Mon, 19 Oct 2026 00:00:00 GMT

Rotylenchulus reniformis (the reniform nematode) is an emerging pest of sweetpotato. The aim of this study is to (i) determine the host status of sweetpotato cultivars to R. reniformis, (ii) identify potential sources of resistance among sweetpotato germplasm, and (iii) evaluate R. reniformis parasitism on root biomass partitioning. Greenhouse trials evaluated R. reniformis reproduction and sweetpotato root weight with 10 commercial cultivars and 28 plant introductions (PIs). All commercial cultivars supported similar nematode reproduction to the susceptible control ‘Beauregard’, indicating widespread susceptibility. Four PIs (‘Saing-Mi’, ‘Regal’, ‘Morada Sombica’, and ‘Tainung 63’) had lower nematode reproduction compared to ‘Beauregard’. Rotylenchulus reniformis infection increased fibrous root biomass by 28.4%, yet reduced storage root weight by an average of 30.4%, demonstrating yield penalties associated with R. reniformis parasitism. This is the first report identifying ‘Covington’ and ‘Orleans’ as highly susceptible to R. reniformis and confirms that R. reniformis significantly inhibits storage root production. These findings emphasize the negative impact of R. reniformis in sweetpotato production and highlight the lack of available host resistance.

Beyond contact toxicity: Volatile and systemic nematode suppression by ‘Candidatus Pseudomonas auctus’ JDE115 in soybean

Mon, 19 Oct 2026 00:00:00 GMT

Plant-parasitic nematodes such as soybean cyst nematodes (SCNs) (Heterodera glycines) and root-knot nematodes (Meloidogyne spp.) pose a significant threat to global soybean productivity. This study evaluated the multimodal biocontrol potential of ‘Candidatus Pseudomonas auctus’ JDE115, a soybean nodule-derived bacterium known for its plant growth-promoting properties. Greenhouse-based pot and split-root assays showed that JDE115 not only significantly improved soybean growth and nodulation but also suppressed SCN reproduction by over 90% locally and 85% systemically, indicating both direct antagonism and possibly induced systemic resistance. Volatile profiling using solid-phase microextraction gas chromatography–mass spectrometry identified dimethyl disulfide and 1-undecene as distinctive volatile organic compounds produced by JDE115. Bioassays with free-living nematodes demonstrated that 1-undecene elicits strong avoidance behavior and causes rapid mortality in nematodes in the absence of direct contact. Furthermore, a colorimetric picrate assay confirmed substantial hydrogen cyanide production by the bacterium. Additional tests revealed that JDE115 inhibits egg hatching and causes juvenile mortality through a combination of chemical toxicity and enzymatic degradation. Collectively, these findings establish JDE115 as a multifunctional plant growth-promoting rhizobacterium with potential nematicidal activity and broad biocontrol potential, underscoring its value as a next-generation microbial tool for integrated nematode management in soybean cultivation. To our knowledge, this is the first report demonstrating combined volatile-mediated and systemic suppression of SCN by a nodule-derived Pseudomonas.

Survey of plant-parasitic nematodes in Arkansas and Mid-South Golf course putting greens

Mon, 19 Oct 2026 00:00:00 GMT

Plant-parasitic nematodes (PPNs) pose a significant risk to turfgrass health, especially golf course putting greens. Several PPN taxa are responsible for causing damage, each varying in their geographic distribution, host preference, and damage potential. Limited information is available on PPN species and distribution within putting greens throughout Arkansas and the surrounding states. In 2023 and 2024, a survey was conducted on 352 greens from 39 golf courses in Arkansas, Louisiana, Mississippi, Tennessee, and Texas. Greens consisted of either creeping bentgrass (Agrostis stolonifera) or hybrid bermudagrass (Cynodon dactylon x. C. transvaalensis). For each soil sample, nematodes were identified to the genus level, and soil characteristics (organic matter, pH, phosphorous, potassium, calcium, magnesium, sulfur, sodium, iron, manganese, zinc, copper, and boron) were measured. A total of 13 PPN taxa were identified across all samples, with spiral (Helicotylenchus spp.) and ring (Criconemoides spp.) nematodes being the most common. The sting nematode (Belonolaimus spp.), a highly destructive PPN, was identified for the first time in Arkansas and Mississippi. PerMANOVA analysis revealed a significant effect of soil parameters and turfgrass species on PPN community composition (p < 0.001). Lance (Hoplolaimus spp.) and stunt (Tylenchorhynchus spp.) nematodes were most common in creeping bentgrass greens, while sting and ring nematodes were most common in hybrid bermudagrass greens. Total PPN density was negatively correlated with pH (r = −0.214), potassium (r = −0.163), and magnesium (r = −0.159). Limited relationships were observed between population densities of specific PPN taxa and the soil characteristics measured. This study provides a better understanding of PPN distribution and prevalence in Arkansas and Mid-South golf course putting greens.

Where next for the discipline of nematology? Trends and implications for workforce sustainability, disease diagnostics, and biosecurity

Mon, 19 Oct 2026 00:00:00 GMT

Nematodes are among the most abundant and functionally important organisms on Earth, strongly influencing soil processes, agricultural productivity, and biosecurity systems. Despite nematodes’ increasingly important role in agricultural resilience and national biosecurity, the discipline has experienced sustained institutional decline across North America. This work examines the historical development, peak, and contraction of nematology as a scientific discipline in the United States, with comparative insights from Canada. Drawing on historical records, professional society membership data, faculty demographics, diagnostic infrastructure, and bibliometric analyses, we demonstrate that current nematology capacity is increasingly fragile, geographically concentrated, and insufficient to meet emerging risks. The findings reveal a paradox: while nematode-driven threats to crops, forests, and ecosystems are intensifying due to global trade and environmental change, workforce pipelines, diagnostic capacity, and institutional support continue to erode. Bibliometric trends further suggest that the field’s future visibility depends on cross-disciplinary integration with molecular biology, ecology, and food security research. We argue that nematology constitutes critical scientific infrastructure and that its decline reflects structural disinvestment rather than diminished relevance. Strategic reinvestment in nematology training, diagnostics, and faculty positions is essential in an increasingly high-risk global environment.

Comparisons of nematode assay efficiencies and recommendations across 10 state nematode assay labs utilizing turfgrass

Mon, 19 Oct 2026 00:00:00 GMT

Nematode assay extraction methods vary among state laboratories for quantifying plant-parasitic nematodes (PPNs) from turfgrass samples, which can lead to differences in nematode population estimates and management recommendations for turfgrass. This study compared the extraction efficiencies of 10 state nematode assay labs in the eastern United States using homogenous turfgrass soil samples. Random samples were collected from three locations; homogenized; and distributed to each lab. Three labs used semi-automatic elutriation, while seven employed hand sieving with centrifugation and sugar floatation. Variability in population estimates among state labs was evident in five of six recorded PPN genera by state and four of six by hand-sieving. Hoplolaimus spp. were different when compared by semi-automatic elutriation with all other genera recorded being similar. Despite variations in counts, recommendations for nematode management were generally consistent within each lab. These findings highlight the importance of using the same assay lab over time for reliable nematode monitoring and suggest that automation may reduce the variability in nematode extraction.

Description of Hexamermis philaeni n. sp. (Enoplea: Mermithidae), a parasitoid of the meadow spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) in Italy

Thu, 15 Oct 2026 00:00:00 GMT

A new species of the genus Hexamermis, Hexamermis philaeni sp. n., was described analyzing specimens isolated from nymphs and adults of the meadow spittlebug Philaenus spumarius in Italy. The combination of the following morphological characters identifies this novel species: terminal mouth opening; the presence of six cephalic papillae; six hypodermal cords at midbody; vulvar opening wide and visible without lips, vulvar cone developed, vagina bend at connection with the uterus; spicules paired, developed, separated and equal in size, slightly curved near the distal end and shorter than the width of the body at the level of the cloaca; genital papillae surrounding the cloaca, surpassing the base of the spicules and arranged as follows: 24 pre-cloacal, 5 ad-cloacal, and 50 postcloacal. Bayesian phylogenetic analysis based on 18S data placed H. philaeni within Hexamermis–Agamermis mixed clade. To our knowledge, this work represents the first record of a Hexamermis species parasitizing P. spumarius.

Postinfection development of Rotylenchulus reniformis on resistant Glycine max genotypes

Wed, 14 Oct 2026 00:00:00 GMT

Soybean (Glycine max) lines JTN-5203, PI 404166, and 02011-126 support less root infection by reniform nematode (Rotylenchulus reniformis), but post-infection effects of these lines on the parasite have not been investigated. In this study, post-infection development and fecundity in these lines were compared with that of the susceptible control cultivar Braxton. The infection cycle was divided into early (up to 10 days after inoculation (DAI)) and late (15–30 DAI) phases, during which root-associated nematodes were classified as vermiform, swelling, reniform, or gravid. Repeated experiments were conducted in a growth chamber in containers filled with steam pasteurized soil mix. Seedlings were inoculated with 500 nematodes (mixed vermiform life stages). Containers were arranged in a completely randomized design. As early as 10 DAI, slightly delayed development was observed on resistant lines with half as many gravid nematodes as Braxton. Line 02011-126 had a higher proportion of swelling nematodes than the other resistant lines in late phases, suggesting different resistance mechanisms. All resistant lines inhibited fecundity, with an up to 98% reduction at 30 DAI. In summary, fewer infections, slower development, and reduced reproduction all contribute to resistance in these lines.

The Strongyloides stercoralis nervous system

Wed, 14 Oct 2026 00:00:00 GMT

The skin-penetrating, human-parasitic nematode Strongyloides stercoralis infects over 600 million people worldwide, causing extensive morbidity and socioeconomic burden. Outside the host body, these nematodes usually live in moist soil as infective third-stage larvae (iL3s), a life stage that is considered to be evolutionarily homologous to the dauer stage of free-living nematodes such as Caenorhabditis elegans. S. stercoralis iL3s navigate their local environment and seek out hosts using sensory cues such as heat, host-emitted odorants, and carbon dioxide. Once host contact is made, iL3s actively explore the surface of the skin before penetrating the tissue and entering the body. Inside the body, iL3s navigate to the small intestine, where they live and reproduce as parasitic adults. The nervous system of S. stercoralis orchestrates the various sensory and motor behaviors that are necessary for successful passage through the parasite life cycle. Here, we describe our current understanding of the structure of the nervous system of the S. stercoralis iL3 stage. We highlight the neurons that drive behaviors such as dispersal, host seeking, and skin penetration. Additionally, we identify differences between the nervous systems of S. stercoralis iL3s and C. elegans dauers; these adaptations likely contribute to the differences in behavior and lifestyle between these two species of nematodes.

Evaluating aldicarb and fluazaindolizine for management of Meloidogyne incognita in Arkansas soybean production

Wed, 14 Oct 2026 00:00:00 GMT

Aldicarb and fluazaindolizine were evaluated in-furrow for management of Meloidogyne incognita in two, multi-year field experiments near Kerr, Arkansas. Aldicarb was evaluated in 2017, 2019, and 2022 on the moderately resistant soybean cultivar, Delta Grow DG4940 GLY, as well as the susceptible cultivars, DG4870 RR or DG4880 GLY, depending on the year. Fluazaindolizine was evaluated in 2019, 2020, and 2022 on the susceptible cultivar DG4880 GLY. Aldicarb significantly reduced galling compared to the non-nematicide treated control (NTC) in 2 of 3 years, whereas fluazaindolizine did so in 1 of 3 years. The cultivar by treatment interaction on galling was significant in each year of the aldicarb experiments, but the effect of the nematicides differed by cultivar and year. Despite reductions in galling, only aldicarb + fluopyram protected grain yield compared to the NTC in the 2019 aldicarb experiment. Regardless of nematicide, the moderately resistant cultivar significantly reduced galling and protected yield compared to the susceptible cultivars. Overall, these data show that aldicarb and fluazaindolizine reduce galling by M. incognita; however, the nematicides were not as effective as a moderately resistant cultivar.

Efficacy of the entomopathogenic nematodes on pupal stage of the Oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae) and Codling moth, Cydia pomonella (Lepidoptera: Tortricidae)

Fri, 25 Sep 2026 00:00:00 GMT

Oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) and codling moth, Cydia pomonella (Lepidoptera: Tortricidae) are serious pests of temperate fruit trees such as peach, pear and apple. Control of pupae would reduce or eliminate damage to fruit caused by subsequent generations. The objective of this study was to evaluate the effectiveness of commercially available entomopathogenic nematodes (EPNs) against pupae of G. molesta and C. pomonella under laboratory conditions. In a preliminary assay, a series of application rates of Steinernema carpocapsae and Steinernema feltiae were used against pupae, and on the basis of the results, we selected an application rate of 5,000 infective juveniles/insect to determine the EPN virulence. Due to limited availability of insects, we conducted two rounds of fully replicated virulence experiments. In virulence test in round 1, we tested S. carpocapsae, S. feltiae, and Heterorhabditis bacteriophora against G. molesta and C. pomonella pupae. In round 2, S. carpocapsae was considered a standard (based on the results of round 1 test) and was included with three other species of EPNs: Steinernema riobrave, Steinernema glaseri, and Heterorhabditis indica. Results indicated that all EPNs tested were pathogenic and caused higher mortality than the non-treated control. The highest pupal mortality (60–80%) against both insects at 3 days posttreatment was observed for S. carpocapsae treatments compared with other treatments in both rounds of tests. In conclusion, EPNs have potential to be applied to G. molesta and C. pomonella pupae to efficiently reduce fruit damage and pest populations in the subsequent growing season. Future studies will need to explore the use of EPNs against G. molesta and C. pomonella pupae under different/natural environmental conditions within orchard agroecosystems.

Policy, collaboration, and extension: creating a global framework for plant-parasitic nematode management

Thu, 24 Sep 2026 00:00:00 GMT

The global burden of plant‑parasitic nematodes (PPN) will not be reduced by technological advances alone. Impact depends on policy design, collaboration across institutions, and durable extension (agricultural outreach and technology transfer programs) systems that translate research into practice. This proceeding integrates five perspectives. A Horizon Europe project (NEM‑EMERGE) demonstrates how coordinated work packages (i.e., closely integrated and jointly managed components of the project) that span spatial modelling, heat‑stable resistance, virulence diagnostics, and soil health can be coupled to socio‑economic engagement to drive adoption. A North American public–private partnership against soybean cyst nematode shows how a single, consistent message with behavioural tools and outcome tracking can change practices. An industry view explains how incentive systems (e.g. reduced‑risk fast track) could speed the development of sustainable solutions and outlines legal challenges with respect to the development of integrated solutions. A Brazilian perspective argues that biologicals or chemicals will not perform without attention to soil health and that public–private collaboration can fill gaps in extension. A policy analysis calls for a revival of extension through regional hubs of excellence that work with global hubs and with expanded digital platforms. Recommendations are to fund multi‑actor platforms, invest in diagnostics and adoption science (the study of factors influencing adoption of technologies and management practices), harmonized regulatory frameworks, and scale digital extension in global hotspots.

Understanding the impact of plant-parasitic nematodes on global food security and trade in a changing world

Thu, 24 Sep 2026 00:00:00 GMT

Plant-parasitic nematodes remain a pervasive yet often hidden constraint on crop productivity, smallholder livelihoods, and market access. This proceedings integrates four vantage points. First, a sub-Saharan African perspective describes how intensification, reliance on informal seed systems, and limited diagnostics allow root-knot nematodes (Meloidogyne spp.) and potato cyst nematodes (PCN) to proliferate in vegetables, tubers, and perennials. Second, a global biogeographic analysis maps soil nematode abundance and trophic structure and demonstrates strong associations with soil organic carbon and temperature, allowing climate-informed risk anticipation. Third, a state-level case study from North Carolina details the response to Meloidogyne enterolobii through interior quarantine, commodity-aligned outreach, and a dedicated quarantine nursery that de-risks field research. Fourth, a South American regulatory perspective from Chile illustrates PCN regionalization and an emergency response to Aphelenchoides fragariae in strawberry, which balanced risk management and socio-economic realities. Cross-cutting priorities include climate-informed surveillance, high-throughput diagnostics, clean seed and nursery certification, and risk-proportionate quarantine aligned with trade. Recommendations for policymakers include coordinated monitoring, harmonized diagnostics, grower-centric extension, and enabling policy frameworks for integrated nematode management and trade resilience.

Rethinking control strategies for plant-parasitic nematodes

Thu, 24 Sep 2026 00:00:00 GMT

To improve management for plant-parasitic nematodes, new solutions must reflect biological realities and be guided by coherent, science-based regulations. This study addresses five areas: (1) regulatory and market hurdles that slow adaptation of biological control products despite strong safety records and scalable production; (2) microbiome-mediated soil resilience, including nematode–microbiome interactions, suppressive consortia, validated delivery systems, and diagnostics to shift soils toward durable suppression; (3) effective containment for nematodes of regulatory concern, and precise resistance bioengineering through genome editing tools that mirror natural allelic variation, thus, warranting streamlined oversight; (4) omics-enabled platforms to discover and validate RNA interference targets for nematode control in perennial crops, coupled with transgrafting; and (5) advances in affordable RNA-based biopesticides and efforts to strengthen social license. Policymakers should adopt fit-for-purpose, fast-track pathways for low-risk biological and biotechnological products, and expand support for diagnostics, core research, and field validation across production systems. Furthermore, to ensure effective implementation, ongoing communication among regulators, scientists, industry, and growers is essential.

Nematodes on the move: An introduction to the symposium proceedings and policy recommendations

Thu, 24 Sep 2026 00:00:00 GMT

This introductory paper summarizes the proceedings of the “Nematodes on the Move” symposium, highlighting the global impacts of plant-parasitic nematodes on agriculture, recent advances in management strategies, and the urgent need for international collaboration, regulatory modernization, and extension networks to support sustainable food production. Key policy recommendations and future research directions are outlined.

SON2026 Abstracts

Sat, 19 Sep 2026 00:00:00 GMT

Efficacy of succinate dehydrogenase inhibitor nematicides fluopyram and cyclobutrifluram against Hoplolaimus galeatus in Golf Turf

Tue, 15 Sep 2026 00:00:00 GMT

Following the cancellation of fernamiphos, lance nematodes (Hoplolaimus spp.) have become one of the most challenging plant-parasitic nematodes to manage in turfgrass systems. Succinate dehydrogenase inhibitor nematicides are among the most used nematode management tools on golf courses in the United States. Currently, there are two SDHI turfgrass nematicides registered in the United States: the fluopyram nematicide Indemnify, which was launched in 2016, and the cyclobutrifluram nematicide Trefinti, which was launched in 2025. The objective of this research was to compare the efficacy of these two nematicides against the lance nematode Hoplolaimus galeatus in microwell bioassays, greenhouse, and field experiments. Microwell bioassays compared the percent mortality of H. galeatus among the nematicides following exposure to different concentrations of the nematicides at different time intervals. Greenhouse experiments compared the efficacy of the nematicides when applied at field-relevant rates to H. galeatus-infested turfgrass in pots. Finally, the efficacy of the two nematicides for management of H. galeatus was compared in turfgrass field trials. In the bioassay experiment, exposure of H. galeatus to cyclobutrifluram resulted in higher nematode mortality than exposure to fluopyram. The greenhouse experiment demonstrated that cyclobutrifluram at 250 g a.i./ha and above significantly suppressed H. galeatus population density, whereas fluopyram did not. Finally, results from field trials indicate that cyclobutrifluram is an effective treatment for enhancing the health of golf turf infested by H. galeatus. These findings suggest that the cyclobutrifluram nematicide Trefinti exhibits strong efficacy against H. galeatus and could serve as an effective treatment for management of H. galeatus on golf turf.

Survey of soybean cyst nematode population abundance and virulence phenotypes in the Sanjiang Plain of Heilongjiang Province, China

Thu, 30 Jul 2026 00:00:00 GMT

Heilongjiang Province is China’s leading soybean-producing region, where the soybean cyst nematode (SCN; Heterodera glycines Ichinohe) has expanded across an increasing geographic range. Recent surveys reported SCN presence in at least 63 soybean-producing counties; however, eastern production zones remain insufficiently characterized. To evaluate the current distribution, population density, and virulence structure of SCN in the Sanjiang Plain, we collected 186 soil samples from 6 major soybean-producing counties in 2024. Samples represented four dominant soil types: black soil (Phaeozem), meadow soil, dark brown soil (Cambisol), and albic soil. SCN was detected in 93.5% of fields, confirming its widespread establishment. Cyst densities ranged from 2 to 10 cysts per 100 g dry soil, corresponding to 122–579 eggs and juveniles per 100 g soil. Population densities were lower under maize–soybean rotation (204 eggs and juveniles per 100 g soil) than under continuous soybean (252 per 100 g), though the difference was modest. Under continuous soybean cultivation, dark brown soils supported significantly higher densities (420 per 100 g of dry soil) compared with black and meadow soils, suggesting that soil type may strongly modulate SCN abundance within this cropping system. Virulence testing of nine representative populations classified all populations as race 3. Female index values remained below 10% on PI 88788, PI 90763, and Pickett, indicating no detectable shift toward broader virulence in this region. Collectively, these findings indicate that although SCN is widely distributed in the Sanjiang Plain, population pressure and virulence complexity remain moderate relative to western Heilongjiang. The dominance of race 3 suggests that current resistance sources remain effective, providing an opportunity for proactive resistance deployment and integrated management to prevent future virulence evolution in this expanding soybean production region.

Pratylenchus pseudocoffeae Mizukubo, 1992 infecting dahlia (Dahlia hybrida) in the United States expands its known host and geographic ranges

Thu, 30 Jul 2026 00:00:00 GMT

Pratylenchus pseudocoffeae is a migratory endoparasitic nematode with a limited documented geographic distribution and host range. We studied nine putative P. pseudocoffeae populations recovered from symptomatic dahlia (Dahlia hybrida) tubers in Ohio and Pennsylvania and evaluated their pathogenicity. Two populations were selected for integrative characterization using morphological and molecular analysis, whereas the remaining populations were identified molecularly based on two ribosomal DNA loci (i.e., internal transcribed spacer and large subunit regions). All populations were confirmed as P. pseudocoffeae, representing the first report of this species in Ohio and Pennsylvania, and the first report of dahlia as a natural host. Pathogenicity was assessed under greenhouse conditions by inoculating rooted dahlia cuttings with initial soil densities of 0, 7, 14, and 28 nematodes cm−3 soil. Inoculated plants exhibited significant reductions in root biomass (61–75%) relative to noninoculated controls. Taken together, these results demonstrate that dahlia is a suitable host of P. pseudocoffeae, contributing to a better understanding of the distribution of this species in North America, and provide molecular diagnostic data to support accurate nematode identification for future diagnostic and epidemiological studies.

Effect of environmental conditions on fluopyram applied in-furrow on cotton nematodes

Tue, 28 Jul 2026 00:00:00 GMT

Fluopyram is a nematicide for use as an at-plant, in-furrow on cotton (among other crops) in the United States. Trials (33 total) were conducted in cotton fields with and without fluopyram applied at-plant, in-furrow in Meloidogyne incognita and Rotylenchulus reniformis infested fields. Root galling caused by M. incognita was reduced on average by 24% with fluopyram. The amount of rain for the first rain after planting did affect fluopyram activity, with 1, 2, and 3 cm rain predicted to reduce root galling by 15, 26, and 39%, respectively. On average, cotton lint yield in M. incognita tests was 7% higher with fluopyram (1,270 kg lint/ha) compared to no nematicide (1,185 kg lint/ha). Fluopyram did not significantly affect R. reniformis density or cotton lint yield in R. reniformis tests. Lint yield increased as the number of days until the first rain increased, and fluopyram-treated plots yielded more than no nematicide as days until the first rain increased. Lint yield decreased in R. reniformis fields as average air temperature increased (for the first 14 days), but the warmer the temperature, the bigger the difference between fluopyram (higher yields) than no nematicide (lower yields). There is strong evidence, even in a water-limited environment like west Texas, that significant early rains will improve fluopyram reduction of M. incognita galling and increase yield. The impact of fluopyram with R. reniformis is not as clear and trended opposite for beneficial environmental conditions compared to M. incognita.

Evaluation of Glycine max genotypes (maturity groups IV and V) for resistance to Meloidogyne enterolobii via phenotypic and GWAS assays

Fri, 17 Jul 2026 00:00:00 GMT

The production of soybean is a vital industry within North Carolina (NC) and the United States, accounting for millions of dollars in annual revenue, yet is currently under threat due to the presence of Meloidogyne enterolobii. Plant resistance is a crucial management technique for this nematode, but M. enterolobii is known to overcome resistant cultivars that have suppressed infection by other root-knot nematodes such as M. incognita. Therefore, identifying novel resistance genes that work antagonistically to M. enterolobii is paramount. In this study, 198 lines of Glycine max were assessed for resistance to M. enterolobii through a greenhouse assay. During initial screening, soybean lines were inoculated with a NC isolate of M. enterolobii and assessed after 60 days for resistance using three metrics, including root galling severity, reproduction factor, and eggs per gram of root. The ten most resistant soybean lines were then tested in a confirmation screening. Three soybean lines: PI 548555, PI 639740, and PI 556852, were confirmed to be resistant with all three lines resulting in resistance metrics under the defined resistance threshold. The use of these lines could provide much needed support to soybean growers in NC and allow for novel resistance loci or genes to be bred into existing cultivars. A Genome-wide association study analysis was performed using publicly available marker data for resistance traits, but no significant associations were detected in the current analysis.