Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Globodera ellingtonae
By: Chandni Shah and Louise-Marie Dandurand
References
- Ahuja, A., Joshi, V., Singh, G., Kundu, A., Bhat, C. G., Kumar, S., Rao, U. and Somvanshi, V. S. 2021. Rapid and sensitive detection of potato cyst nematode Globodera rostochiensis by loop-mediated isothermal amplification assay. 3 Biotech, 11: 1–8.
- Ahuja, A. and Somvanshi, V. S. 2021. Diagnosis of plant-parasitic nematodes using loop-mediated isothermal amplification (LAMP): A review. Crop Protection, 147:105–459.
- APHIS-USDA. 2009. Best management practices for preventing spread of potato cyst nematodes. Online publication. United States Department of Agriculture’s Animal and Plant Health Inspection Service. https://www.aphis.usda.gov/sites/default/files/bestpractices.pdf
- Babilônia, G. B., Lopes, E. A., Londe, M. L. A., Ribeiro, L. B., Oliveira, C. M. G. and Buonicontro, D. S. 2024. Unveiling the potential of loop-mediated isothermal amplification (LAMP) for plant-parasitic nematode identification: a review. Journal of Plant Diseases and Protection, 131:665–679
- Bairwa, A., Dipta, B., Verma, G., Venkatasalam, E. P., Shanthi, A., Jeevalatha, A., Naga, K. C., Sharma, S., Thakur, D. and Mhatre, P. H. 2023. Development and evaluation of loop-mediated isothermal amplification assay for rapid and sensitive detection of potato cyst nematode, Globodera pallida from soil. 3 Biotech, 13:1–10.
- Bekal, S., Niblack, T. L. and Lambert, K. N. 2003. A chorismate mutase from the soybean cyst nematode Heterodera glycines shows polymorphisms that correlate with virulence. Molecular Plant-Microbe Interactions, 16:439–446.
- Camacho, M. J., Albuquerque, D. C., Inácio, M. L., Martins, V. C., Mota, M., Freitas, P. P. and de Andrade, E., 2024. FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—The pale potato cyst nematode. Frontiers in Bioengineering and Biotechnology, 12:78–79.
- Chronis, D., Chen, S., Skantar, A. M., Zasada, I. A. and Wang, X. 2014. A new chorismate mutase gene identified from Globodera ellingtonae and its utility as a molecular diagnostic marker. European Journal of Plant Pathology, 139:239–246.
- Contina, J. B., Dandurand, L. M. and Knudsen, G. R. 2019. A predictive risk model analysis of the potato cyst nematode Globodera pallida in Idaho. Plant Disease, 103:3117–3128.
- Dandurand, L. M. and Knudsen, G. R. 2016. Effect of the trap crop Solanum sisymbriifolium and two biocontrol fungi on reproduction of the potato cyst nematode, Globodera pallida. Annals of Applied Biology, 169:180–189.
- Ding, S. W., Yang, S. H., Wu, W. J., Xie, H. and Xu, C. L. 2019. Rapid diagnosis of Ditylenchus destructor by loop-mediated isothermal amplification assay based on 28S rRNA sequences. European Journal of Plant Pathology, 153:1165–1175.
- Ezzatyhusna, N., Nurul, M., Siti, I. H., Mn, S. and Suharni. 2017. Sensitivity evaluation of SYBR green I, SYBR safe and calcein dyes for detection of human papillomavirus 16 by loop-mediated isothermal amplification. Medicine and Health, 12(2):220–229.
- Handoo, Z. A., Carta, L. K., Skantar, A. M. and Chitwood, D. J. 2012. Description of Globodera ellingtonae n. sp. (Nematoda: Heteroderidae) from Oregon. Journal of Nematology, 44:40–57.
- Hesse, C. N., Moreno, I., Pardo, O. A., Fuentes, H. P., Grenier, E., Dandurand, L. M. and Zasada, I. A. 2021. Characterization of Globodera ellingtonae populations from Chile utilizing whole genome sequencing. Journal of Nematology, 53:2021–2088.
- Jones, J. T., Haegeman, A., Danchin, E. G. J., Gaur, H. S., Helder, J., Jones, M. G. K., Kikuchi, T., Manzanilla-López, R., Palomares-Rius, J. E., Wesemael, W. M. L. and Perry, R. N. 2013. Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, 14:946–961.
- Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N. and Kanzaki, N. 2009. A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology, 99: 1365–1369.
- Lax, P., Rondan Dueñas, J. C., Franco-Ponce, J., Gardenal, C. N. and Doucet, M. E. 2014. Morphology and DNA sequence data reveal the presence of Globodera ellingtonae in the Andean region. Contributions to Zoology, 83:227–243.
- Madani, M., Ward, L. J. and De Boer, S. H. 2008. Multiplex real-time polymerase chain reaction for identifying potato cyst nematodes, Globodera pallida and Globodera rostochiensis and the tobacco cyst nematode, Globodera tabacum. Canadian Journal of Plant Pathology, 30:554–564.
- Mhatre, P. H., Divya, K. L., Venkatasalam, E. P., Watpade, S., Bairwa, A. and Patil, J. 2022. Management of potato cyst nematodes with special focus on biological control and trap cropping strategies. Pest Management Science, 78:3746–3759.
- Nakhla, M. K., Owens, K. J., Li, W., Wei, G., Skantar, A. M. and Levy, L. 2010. Multiplex real-time PCR assays for the identification of the potato cyst and tobacco cyst nematodes. Plant Disease, 94:959–965.
- National Agricultural Statistics Service. 2023. https://esmis.nal.usda.gov/sites/default/release-files/fx719m44h/cr56pj02j/4q77h840g/pots0923.pdf 1–22.
- Nisa, R. U., Tantray, A. Y. and Shah, A. A. 2022. Shift from morphological to recent advanced molecular approaches for the identification of nematodes. Genomics, 114:110–295.
- Niu, J. H., Guo, Q. X., Jian, H., Chen, C. L., Yang, D., Liu, Q., & Guo, Y. D. 2011. Rapid detection of Meloidogyne spp. by LAMP assay in soil and roots. Crop Protection, 30:1063–1069.
- Notomi, T., Mori, Y., Tomita, N. and Kanda, H. 2015. Loop-mediated isothermal amplification (LAMP): principle, features and future prospects. Journal of Microbiology, 53:1–5.
- Nour, S. M., Lawrence, J. R., Zhu, H., Swerhone, G. D. W., Welsh, M., Welacky, T. W. and Topp, E. 2003. Bacteria associated with cysts of the soybean cyst nematode (Heterodera glycines). Applied and Environmental Microbiology, 69:607–615.
- Phillips, W. S., Kitner, M. and Zasada, I. A. 2017. Developmental dynamics of Globodera ellingtonae in field-grown potato. Plant Disease, 101:1182–1187.
- Price, J. A., Coyne, D., Blok, V. C. and Jones, J. T. 2021. Potato cyst nematodes Globodera rostochiensis and G. pallida. Molecular Plant Pathology, 22:495–507.
- Ruang-areerate, T., Sukphattanaudomchoke, C., Thita, T., Leelayoova, S., Piyaraj, P., Mungthin, M., Suwannin, P., Polpanich, D., Tangchaikeeree, T., Jangpatarapongsa, K., Choowongkomon, K. and Siripattanapipong, S. 2021. Development of loop-mediated isothermal amplification (LAMP) assay using SYBR safe and gold-nanoparticle probe for detection of Leishmania in HIV patients. Scientific Reports, 11:12152.
- Singh, G., Ahuja, A., Rao, U. and Somvanshi, V. S. 2021. Loop-mediated isothermal amplification based identification of entomopathogenic nematodes Heterorhabditis spp. and Steinernema spp. from soil DNA. BioControl, 66: 701–712.
- Skantar, A. M., Handoo, Z. A., Zasada, I. A., Ingham, R. E., Carta, L. K. and Chitwood, D. J. 2011. Morphological and molecular characterization of Globodera populations from Oregon and Idaho. Phytopathology, 101: 480–491.
- Sriworarat, C., Phumee, A., Mungthin, M., Leelayoova, S. and Siriyasatien, P. 2015. Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection. Parasites and Vectors, 8:591.
- Subbotin, S. A. and Franco, J. 2022. Molecular characterisation of the sedentary cystoid nematode, Mesodolichodera andina (Golden, Franco, Jatala and Astogaza, 1983) gen. n., comb. n. (Tylenchida: Heteroderidae) parasitising potatoes in the Andes. Russian Journal of Nematology, 30:31–42.
- Subbotin, S. A., Franco, J., Knoetze, R., Roubtsova, T. V., Bostock, R. M. and Cid Del Prado Vera, I. 2020. DNA barcoding, phylogeny and phylogeography of the cyst nematode species from the genus Globodera (Tylenchida: Heteroderidae). Nematology, 22:269–297.
- Subbotin, S. A., Vierstraete, A., De Ley, P., Rowe, J., Waeyenberge, L., Moens, M. and Vanfleteren, J. R. 2001. Phylogenetic relationships within the cyst-forming nematodes (Nematoda, Heteroderidae) based on analysis of sequences from the ITS regions of ribosomal DNA. Molecular Phylogenetics and Evolution, 21:1–16.
- Sukphattanaudomchoke, C., Siripattanapipong, S., Thita, T., Leelayoova, S., Piyaraj, P., Mungthin, M. and Ruang-areerate, T. 2020. Simplified closed tube loop mediated isothermal amplification (LAMP) assay for visual diagnosis of Leishmania infection. Acta Tropica, 212:105–651.
- Suwanngam, A., Schiffer, P. H., Sasnarukkit, A., Siripattanapipong, S., Jindapunnapat, K., Chinnasri, B. and Ruang-areerate, T. 2025. Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues. Scientific Reports, 15:1–12.
- Suwannin, P., Polpanich, D., Leelayoova, S., Mungthin, M., Tangboriboonrat, P., Elaissari, A., Jangpatarapongsa, K., Ruang-areerate, T. and Tangchaikeeree, T. 2021. Heat-enhancing aggregation of gold nanoparticles combined with loop-mediated isothermal amplification (HAG-LAMP) for Plasmodium falciparum detection. Journal of Pharmaceutical and Biomedical Analysis, 203:14–178.
- Talavera, M., Andreu, M., Valor, H. and Tobar, A. 1998. Nematodos fitoparásitos en áreas productoras de patata de Motril y Salobreña. Investigación Agraria. Producción y Protección Vegetales, 13:87–95.
- Thita, T., Leelayoova, S., Mungthin, M. and Ruang-Areerate, T. 2019. Reliable interpretation and long-term stability using SYBR TM safe fluorescent assay for loop-mediated isothermal amplification (LAMP) detection of Leishmania spp. Tropical Biomedicine, 36:495–504.
- USDA-APHIS. 2009a. Best management practices for preventing spread of potato cyst nematodes. Online publication. United States Department of Agriculture s Animal and Plant Health Inspection Service. https://www.aphis.usda.gov/sites/default/files/bestpractices.pdf
- USDA-APHIS. 2009b. Pale potato cyst nematode national survey and diagnostic cyst sample forwarding protocols. United States Department of Agriculture’s Animal and Plant Health Inspection Service. https://www.aphis.usda.gov/sites/default/files/PCN_NatlSurvey.pdf
- Vallejo, D., Rojas, D. A., Martinez, J. A., Marchant, S., Holguin, C. M. and Pérez, O. Y. 2021. Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia. PLoS One, 16:1–20.
- Vieira, P. and Gleason, C. 2019. Plant-parasitic nematode effectors — insights into their diversity and new tools for their identification. Current Opinion in Plant Biology, 50:37–43.
- Wainer, J. and Dinh, Q. 2021. Taxonomy, morphological and molecular identification of the potato cyst nematodes, Globodera pallida and G. rostochiensis. Plants, 10:1–21.
- Xu, C., Yang, S., Xie, Y., He, J., Chen, Y., Pan, Y. and Xie, H. 2023. Morphological and molecular characterization, including parasitic and pathogenic studies of a new spherical cyst nematode species, Globodera vulgaris n. sp. (Nematoda: Heteroderidae), associated with potatoes in China. Phytopathology, 113: 1560–1582.
- Yang, G., Li, B., Liu, L., Huang, W., Zhang, W. and Liu, Y. 2012. Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of a new SFTS bunyavirus. Archives of Virology, 157:1779–1783.
- Yang, S., Li, J., Yang, S., Tang, S., Wang, H., Xu, C. and Xie, H. 2024. A chorismate mutase from Radopholus similis plays an essential role in pathogenicity. Journal of Integrative Agriculture, 23:923–937.
- Yu, H., Chronis, D., Lu, S. and Wang, X. 2011. Chorismate mutase: An alternatively spliced parasitism gene and a diagnostic marker for three important Globodera nematode species. European Journal of Plant Pathology, 129:89–102.
- Yu, L. Z., Song, S. Y., Yu, C., Qi, L. J., Yu, Z. X., Jiao, B. B. and Yang, J. 2018. A loop mediated isothermal amplification (LAMP) assay for rapid and reliable detection of Anguina wevelli, a grass parasitic nematode. European Journal of Plant Pathology, 150:725–734.
- Zasada, I. A., Ingham, R. E., Baker, H. and Phillips, W. S. 2019. Impact of Globodera ellingtonae on yield of potato (Solanum tuberosum). Journal of Nematology, 51:e2019–73.
- Zhang, L. and Gleason, C. 2019. Loop-mediated isothermal amplification for the diagnostic detection of Meloidogyne chitwoodi and M. Fallax. Plant Disease, 103:12–18.
- Zhou, Q. J., Cai, Y., Gu, J. F., Wang, X. and Chen, J. 2017. Rapid and sensitive detection of Meloidogyne mali by loop-mediated isothermal amplification combined with a lateral flow dipstick. European Journal of Plant Pathology, 148:755–769.
Language: English
Page range: 208 - 219
Submitted on: Mar 30, 2026
Accepted on: May 23, 2026
Published on: Jul 7, 2026
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Related subjects:
© 2026 Chandni Shah, Louise-Marie Dandurand, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.