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Influence of nanoemulsion on the adhesion and survival of Aeromonas sp. in meat and contact surfaces of meat Cover

Influence of nanoemulsion on the adhesion and survival of Aeromonas sp. in meat and contact surfaces of meat

Open Access
|Jan 2021

Figures & Tables

Figure 1

Phylogenic analysis of the isolated strain Aeromonas sp. AUBAS34 along with its neighbours with a bootstrap value of 1000

Abbildung 1. Phylogene Analyse des isolierten Stammes Aeromonas sp. AUBAS34 zusammen mit seinen Nachbarn mit einem Bootstrap-Wert von 1000

Table 1

Influence of nanoemulsion treatment on the hydrophobicity and biofilm formation of Aeromonas sp.

Tabelle 1. Veränderungen der Hydrophobizität und Biofilmbildung von Aeromonas sp. durch die Nanoemulsionsbehandlung

TreatmentHydrophobicity (SAT)Swarming motility % surface coverageBiofilm formation (OD 595 nm)
AUSN3>1.617.5 ± 2.4c0.54 ± 0.18c
Preservative1.229.6 ±3.2b0.94 ± 0.12b
Control0.834.9 ± 2.7a1.42 ± 0.22a

[i] Hydrophobicity determined according to SAT (salt aggregation test). Biofilm formation using microtiter plate assay. Swarming motility in the presence of 0.5% agar. Different letters after values indicate that there is a significant difference at p < 0.05.

Table 2

Influence of AUSN3 treatment on the adhesion of Aeromonas sp. to meat and contact surfaces of meat

Tabelle 2. Einfluss der AUSN3-Behandlung auf die Adhäsion von Aeromonas sp. zu Fleisch und Kontaktflächen von Fleisch

Cultivation mediaTreatmentNumber of cells adhered (log CFU/g)*
Meat surfaceContact surface of meat
BeefMuttonPorkPolystyreneGlassStainless steel
PCA agarAUSN35.8 ± 0.03c5.5 ± 0.11b5.6 ± 0.04c4.3 ± 0.10c3.4 ± 0.0b3.2 ± 0.1b
Preservative6.4 ± 0.04b5.9 ± 0.08a,b6.4 ± 0.10b5.2 ± 0.08b4.9 ± 0.4a5.5 ± 0.1a
Control7.2 ± 0.10a6.3 ± 0.44a7.1 ± 0.12a6.1 ± 0.10a5.3 ± 0.1a5.7 ± 0.0 a

* No. of bacteria that adhered after a time period of 2 h at 20 °C. Different letters after values indicate that there is a significant difference at p < 0.05.

Figure 2

Influence of AUSN3 on the survival of Aeromonas sp. in meat and contact surfaces of meat at 20 ± 2 °C. a) polystyrene, b) glass, c) stainless steel, d) beef, e) pork and f) mutton. Values are a means of three replications ± standard deviations.

Abbildung 2. Einfluss von AUSN3 auf das Überleben von Aeromonas sp. in Fleisch und Kontaktflächen von Fleisch bei 20 ± 2 ° C. a) Polystyrol, b) Glas, c) Edelstahl, d) Rindfleisch, e) Schweinefleisch und f) Hammel. Die Werte sind Mittelwerte aus drei Wiederholungen ± Standardabweichungen.

DOI: https://doi.org/10.2478/boku-2020-0012 | Journal eISSN: 2719-5430 (formerly 0006-5471) | Journal ISSN: 0006-5471
Language: English, German
Page range: 137 - 145
Submitted on: Apr 28, 2020
Accepted on: Aug 31, 2020
Published on: Jan 29, 2021
Published by: Universität für Bodenkultur Wien
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2021 Melvin Joe Manoharan, Kanakambaram Bradeeba, Abitha Benson, Palanivel Karpagavinayaga Sivakumaar, published by Universität für Bodenkultur Wien
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.