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Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture Cover

Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Medical Journal of Cell Biology
Volume 8 (2020): Issue 4 (December 2020)
By: Artur Bryja,  Wojciech Pieńkowski,  Katarzyna Stefańska,  Błażej Chermuła,  Rut Bryl,  Maria Wieczorkiewicz,  Jakub Kulus,  Grzegorz Wąsiatycz,  Dorota Bukowska,  Kornel Ratajczak,  Jędrzej M. Jaśkowski,  James N. Petitte,  Paul Mozdziak,  Leszek Pawelczyk,  Robert Z. Spaczyński and  Paweł Antosik  
Open Access
|Dec 2020

Figures & Tables

Figure 1

TGFB 1, CD105 and FSP1 expression in granulosa cells during 7-day in vitro culture. Data was presented like logarithm of fold change. Statistical analysis of the data was performed using the T-test and the differences were considered to be significant at the level of p<0.001 (***), p<0.01 (**), p<0.05 (*)
TGFB 1, CD105 and FSP1 expression in granulosa cells during 7-day in vitro culture. Data was presented like logarithm of fold change. Statistical analysis of the data was performed using the T-test and the differences were considered to be significant at the level of p<0.001 (***), p<0.01 (**), p<0.05 (*)

Oligonucleotide sequences of primers used for RT-qPCR analysis

GENEGENE ACCESSION NUMBERPRIMER SEQUENCE (5’-3’)PRODUCT SIZE (BP)
TGFB1NM_000660CCTTGCGGAAGTCAATGTACA GGCCTTTCCTGCTTCTCATG150
CD(ENG) 105BC014271CACTAGCCAGGTCTCGAAGG CTGAGGACCAGAAGCACCTC165
(SFSP1001 A4)CR450345TCTTGGGGAAAAGGACAGATGA CCCAACCACATCAGAGGAGT195
ACTBNM_001101CTCAGGAGGAGCAATGATCTTG AAAGACCTGTACGCCAACAC132
DOI: https://doi.org/10.2478/acb-2020-0019 | Journal eISSN: 2544-3577
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Language: English
Page range: 152 - 157
Submitted on: Oct 6, 2020
Accepted on: Nov 17, 2020
Published on: Dec 31, 2020
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
granulosa cells,
TGFB1,
CD105,
FSP1
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2020 Artur Bryja, Wojciech Pieńkowski, Katarzyna Stefańska, Błażej Chermuła, Rut Bryl, Maria Wieczorkiewicz, Jakub Kulus, Grzegorz Wąsiatycz, Dorota Bukowska, Kornel Ratajczak, Jędrzej M. Jaśkowski, James N. Petitte, Paul Mozdziak, Leszek Pawelczyk, Robert Z. Spaczyński, Paweł Antosik, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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