Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Bryja, Artur; Pieńkowski, Wojciech; Stefańska, Katarzyna; Chermuła, Błażej; Bryl, Rut; Wieczorkiewicz, Maria; Kulus, Jakub; Wąsiatycz, Grzegorz; Bukowska, Dorota; Ratajczak, Kornel; Jaśkowski, Jędrzej M.; Petitte, James N.; Mozdziak, Paul; Pawelczyk, Leszek; Spaczyński, Robert Z.; Antosik, Paweł

doi:10.2478/acb-2020-0019

Abstract

The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.

These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells.

Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone.

Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique.

Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed.

Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells

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Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

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