Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Artur Bryja; Wojciech Pieńkowski; Katarzyna Stefańska; Błażej Chermuła; Rut Bryl; Maria Wieczorkiewicz; Jakub Kulus; Grzegorz Wąsiatycz; Dorota Bukowska; Kornel Ratajczak; Jędrzej M. Jaśkowski; James N. Petitte; Paul Mozdziak; Leszek Pawelczyk; Robert Z. Spaczyński; Paweł Antosik

doi:10.2478/acb-2020-0019

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Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Medical Journal of Cell Biology

Volume 8 (2020): Issue 4 (December 2020)

By: Artur Bryja, Wojciech Pieńkowski, Katarzyna Stefańska, Błażej Chermuła, Rut Bryl, Maria Wieczorkiewicz, Jakub Kulus, Grzegorz Wąsiatycz, Dorota Bukowska, Kornel Ratajczak, Jędrzej M. Jaśkowski, James N. Petitte, Paul Mozdziak, Leszek Pawelczyk, Robert Z. Spaczyński and Paweł Antosik

Open Access

|Dec 2020

Abstract

The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.

These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells.

Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone.

Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique.

Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed.

Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells

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Articles in this issue

DOI: https://doi.org/10.2478/acb-2020-0019 | Journal eISSN: 2544-3577

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Language: English

Page range: 152 - 157

Submitted on: Oct 6, 2020

Accepted on: Nov 17, 2020

Published on: Dec 31, 2020

Published by: Foundation for Cell Biology and Molecular Biology

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

granulosa cells,

TGFB1,

CD105,

Related subjects:

© 2020 Artur Bryja, Wojciech Pieńkowski, Katarzyna Stefańska, Błażej Chermuła, Rut Bryl, Maria Wieczorkiewicz, Jakub Kulus, Grzegorz Wąsiatycz, Dorota Bukowska, Kornel Ratajczak, Jędrzej M. Jaśkowski, James N. Petitte, Paul Mozdziak, Leszek Pawelczyk, Robert Z. Spaczyński, Paweł Antosik, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 8 (2020): Issue 4 (December 2020)