Fig. 1.
Fig. 1.
Fig. 2.
Tirandamycin biosynthesis-related gene function prediction and consistency analysis_
| Number | Gene lengths (bp) | Coding protein | Homologous strain | Resemblance (%) |
|---|---|---|---|---|
| 4283 | 645 | TamK (TetR family regulator) | Streptomyces sp. 307-9 | 99 |
| 4284 | 1494 | TamJ (efflux pump) | Streptomyces sp. 307-9 | 99 |
| 4285 | 1236 | TamI (cytochrome P450) | Streptomyces sp. 307-9 | 99 |
| 4286 | 2814 | TamH (LuxR family regulator) | Streptomyces sp. 307-9 | 97 |
| 4287 | 2094 | TamG (DNA helicase) | Streptomyces sp. 307-9 | 99 |
| 4288 | 1539 | TamF (Prenyltransferase) | Streptomyces sp. 307-9 | 97 |
| 4289 | 831 | TamE (glucosidase) | Streptomyces sp. 307-9 | 98 |
| 4290 | 3165 | TamD (NRPS) | Streptomyces sp. 307-9 | 97 |
| 4291 | 831 | TamC (hypothetical protein) | Streptomyces sp. 307-9 | 97 |
| 4292 | 777 | TamB (alpha/beta fold hydrolase) | Streptomyces sp. 307-9 | 98 |
| 4293 | 8400 | TamAIII | Streptomyces sp. 307-9 | 95 |
| 4294 | 10131 | TamAII | Streptomyces sp. 307-9 | 94 |
| 4295 | 17490 | TamAI | Streptomyces sp. 307-9 | 95 |
| 4296 | 1503 | TamL (FAD-binding oxidoreductase) | Streptomyces sp. 307-9 | 98 |
| 4297 | 234 | TamM (4’-phosphopantetheinyl transferase) | Streptomyces. sp. 307-9 | 97 |
| 4291 | 831 | TamC (hypothetical protein) | Streptomyces sp. 307-9 | 97 |
Antagonistic effect of compound 44567-1 against Pseudomonas aeruginosa_
| Concentration (μg/ml) | 256 | 128 | 64 | 32 | 16 | 8 | 4 | 2 | 1 | 0.5 | 0.25 | 0 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| growth state | - | - | - | - | - | - | + | ++ | +++ | +++ | +++ | +++ |
TRM44567 secondary metabolite gene cluster prediction_
| Number | Gene cluster position | Lengths (bp) | Typology | Similar gene clusters | Resemblance (%) |
|---|---|---|---|---|---|
| 1 | 8,773-35,720 | 26,948 | butyrolactone, terpene | 2-methylisoborneol | 75 |
| 2 | 214,388-239,491 | 25,104 | lanthipeptide-class-i | - | - |
| 3 | 667,293-708,315 | 41,023 | other | 7-deoxypactamycin | 11 |
| 4 | 744,740-767,544 | 22,805 | lanthipeptide-class-iv | venezuelin | 50 |
| 5 | 811,611-837,822 | 26,212 | amglyccycl, tyrolactone | acarbose | 14 |
| 6 | 1,098,443-1,154,095 | 55,653 | T1PKS, NRPS | jomthonic acid A/B/C | 5 |
| 7 | 1,329,308-1,339,462 | 10,155 | RiPP-like | - | - |
| 8 | 1,355,940-1,397,127 | 41,188 | T3PKS | gobichelin A/B | 16 |
| 9 | 1,541,462-1,565,687 | 24,226 | lanthipeptide-class-i | - | - |
| 10 | 2,467,823-2,476,751 | 8,929 | RiPP-like | - | - |
| 11 | 2,621,822-2,641,748 | 19,927 | Trpene | albaflavenone | 100 |
| 12 | 3,407,363-3,468,045 | 60,683 | siderophore, lipolanthine, T1PKS, lanthipeptide-class-iii, linaridin | kinamycin | 22 |
| 13 | 3,603,223-3,671,199 | 67,977 | NRPS, NRPS-like | thiocoraline | 60 |
| 14 | 3,715,948-3,744,688 | 28,741 | RiPP-like, terpene | ashimides | 12 |
| 15 | 3,772,034-3,793,450 | 21,417 | butyrolactone | γ-butyrolactone | 100 |
| 16 | 3,859,629-3,900,359 | 40,731 | NRPS, NAPAA | stenothricin | 13 |
| 17 | 4,043,123-4,056,246 | 13,124 | siderophore | atakemycin | 6 |
| 18 | 4,127,840-4,168,065 | 40,226 | NRPS-like | lobosamide A/B/C | 4 |
| 19 | 4,646,047-4,672,724 | 26,678 | terpene | hopene | 92 |
| 20 | 4,773,585-4,854,740 | 81,156 | NRPS, T1PKS | tirandamycin | 100 |
| 21 | 5,069,238-5,079,453 | 10,216 | Ripp-like | informatipeptin | 42 |
| 22 | 276,872-347,373 | 70,502 | T2PKS | prejadomycin/gaudimyc in | 29 |
| 23 | 776,140-787,912 | 11,773 | siderophore | desferrioxamin B/E | 83 |
| 24 | 905,539-914,564 | 9,026 | melanin | melanin | 60 |
| 25 | 1,263,341-1,304,207 | 40,867 | T3PKS | s56-p1 | 11 |
| 26 | 1,631,756-1,704,267 | 72,512 | T2PKS | spore pigment | 83 |
| 27 | 2,082,866-2,093,270 | 10,405 | ectoine | ectoine | 100 |
| 28 | 2,295,368-2,314,558 | 19,191 | CDPS | - | - |
| 29 | 2,369,855-2,402,869 | 33,015 | NAPAA | meilingmycin | 2 |
| 30 | 2,970,457-3,010,999 | 40,543 | T3PKS | herboxidiene | 7 |
| 31 | 3094,635-3,115,642 | 21,008 | terpene | desotamide | 9 |
| 32 | 3,430,099-3,508,231 | 78,133 | Melanin, T1PKS, NRPS | scabichelin | 100 |
| 33 | 3,579,258-3,620,409 | 41,152 | T3PKS | alkylresorcinol | 100 |
| 34 | 3,887,970-3,908,680 | 20,711 | CDPS | - | - |
| 35 | 3,920,922-3,964,188 | 43,267 | NRPS, NAPAA | stenothricin | 13 |
Comparison of the antibacterial activities of 44567-1 against different targets_
| Number | Target organism | Bacteriostatic activity screening (diameter of inhibition circle) |
|---|---|---|
| 1 | Escherichia coli ATCC® 25922™ | - |
| 2 | Staphylococcus aureus ATCC® 25923™ | 3.0 ± 0.2 mm |
| 3 | Enterococcus faecalis ATCC® 29212™ | - |
| 4 | Klebsiella pneumoniae ATCC® 10031™ | - |
| 5 | Acinetobacter baumannii ATCC® 19606™ | - |
| 6 | Pseudomonas aeruginosa ATCC® 27853™ | 32.0 ± 0.3 mm |
| 7 | Salmonella choleraesu | - |
| 8 | Salmonella castellani | - |
| 9 | Candida albicans ATCC® 64550™ | - |