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Transcriptome Analysis of Komagataeibacter europaeus CGMCC 20445 Responses to Different Acidity Levels During Acetic Acid Fermentation Cover

Transcriptome Analysis of Komagataeibacter europaeus CGMCC 20445 Responses to Different Acidity Levels During Acetic Acid Fermentation

Polish Journal of Microbiology
Volume 70 (2021): Issue 3 (September 2021)
By: LITING WANG,  HOUSHENG HONG,  CHENGBO ZHANG,  ZUNXI HUANG and  HUIMING GUO  
Open Access
|Sep 2021

Figures & Tables

Fig. 1.

Acetic acid fermentation of Komagataeibacter europaeus CGMCC 20445 at an initial ethanol concentration of 4.3% (v/v).A) The cell growth of K. europaeus CGMCC 20445. B) Changes in the acidity of the fermentation medium. The arrow indicates the sample corresponding to the transcriptome sequencing. Data obtained from three biological replicates were shown as mean ± standard deviation.
Acetic acid fermentation of Komagataeibacter europaeus CGMCC 20445 at an initial ethanol concentration of 4.3% (v/v).A) The cell growth of K. europaeus CGMCC 20445. B) Changes in the acidity of the fermentation medium. The arrow indicates the sample corresponding to the transcriptome sequencing. Data obtained from three biological replicates were shown as mean ± standard deviation.

Fig. 2.

Heatmap of differentially expressed genes. The values of lg (FPKM+1) of genes in different samples are normalized, and the genes are clustered. The upregulated and downregulated genes were represented by red and blue grids, respectively. Table S3 shows the names of each gene, fkpm, padj, and the annotation information.
Heatmap of differentially expressed genes. The values of lg (FPKM+1) of genes in different samples are normalized, and the genes are clustered. The upregulated and downregulated genes were represented by red and blue grids, respectively. Table S3 shows the names of each gene, fkpm, padj, and the annotation information.

Fig. 3.

The expression status of genes associated with acid resistance for K. europaeus CGMCC 20445 (acidity: 4.08 ± 0.21 g/100 ml). The upregulated and downregulated genes were denoted by red and blue texts, respectively. PQQ-ADH – pyrroloquinoline quinone-dependent alcohol dehydrogenase; PQQ-ALDH – pyrroloquinoline quinone-dependent aldehyde dehydrogenase; NAD-ADH – NAD-dependent alcohol dehydrogenase; NAD-ALDH – NAD-dependent acetaldehyde dehydrogenase.
The expression status of genes associated with acid resistance for K. europaeus CGMCC 20445 (acidity: 4.08 ± 0.21 g/100 ml). The upregulated and downregulated genes were denoted by red and blue texts, respectively. PQQ-ADH – pyrroloquinoline quinone-dependent alcohol dehydrogenase; PQQ-ALDH – pyrroloquinoline quinone-dependent aldehyde dehydrogenase; NAD-ADH – NAD-dependent alcohol dehydrogenase; NAD-ALDH – NAD-dependent acetaldehyde dehydrogenase.

Statistics of differentially expressed genes of the samples treated_

TimeNumber of up-regulated genesNumber of down-regulated genes differentially expressed genes
40 h  502a (14%)406 (12%)  908 (26%)
64 h500 (14%)479 (14%)  979 (28%)
88 h602 (17%)620 (18%)1,222 (35%)
DOI: https://doi.org/10.33073/pjm-2021-027 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 305 - 313
Submitted on: Mar 9, 2021
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Accepted on: Jun 20, 2021
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Published on: Sep 17, 2021
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
acetic acid bacteria,
acid resistance,
transcriptomics
Related subjects:
Life sciences,
Microbiology and virology

© 2021 LITING WANG, HOUSHENG HONG, CHENGBO ZHANG, ZUNXI HUANG, HUIMING GUO, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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