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Isolated Phosphate-Solubilizing Soil Bacteria Promotes In vitro Growth of Solanum tuberosum L. Cover

Isolated Phosphate-Solubilizing Soil Bacteria Promotes In vitro Growth of Solanum tuberosum L.

Polish Journal of Microbiology
Volume 69 (2020): Issue 3 (September 2020)
By: GUSTAVO YAÑEZ-OCAMPO,  MARTHA E. MORA-HERRERA,  ARNOLDO WONG-VILLARREAL,  DENISSE M. DE LA PAZ-OSORIO,  NADIA DE LA PORTILLA-LÓPEZ,  JORGE LUGO,  ROCIO VACA-PAULÍN and  PEDRO DEL ÁGUILA  
Open Access
|Sep 2020

Figures & Tables

Fig. 1.

Phosphate solubilization assay, measured by halo formation by four bacterial strains cultured on PVK agar. The lines above each bar indicate standard deviation. The different letters above the bars denote statistical differences (LSD test (p < 0.001)).
Phosphate solubilization assay, measured by halo formation by four bacterial strains cultured on PVK agar. The lines above each bar indicate standard deviation. The different letters above the bars denote statistical differences (LSD test (p < 0.001)).

Fig. 2.

Kinetics of the bacterial growth on PVK liquid medium.a) the bacterial growth measured as optical density at a wavelength of 600 nm, b) the orthophosphates released measured with molybdenum blue method, and c) the acidification evidence (lower pH values) in PVK liquid medium. The lines shown above or below each point indicate the standard deviation.
Kinetics of the bacterial growth on PVK liquid medium.a) the bacterial growth measured as optical density at a wavelength of 600 nm, b) the orthophosphates released measured with molybdenum blue method, and c) the acidification evidence (lower pH values) in PVK liquid medium. The lines shown above or below each point indicate the standard deviation.

Fig. 3.

Phylogenetic tree of A3 bacterial strain identified by 16 S rDNA molecular analysis.
Phylogenetic tree of A3 bacterial strain identified by 16 S rDNA molecular analysis.

Fig. 4.

Development of adventitious roots in potato microplants (Solanum tuberosum L.) cv. Citlali, inoculated with PSB strain A3 and cultured for 28 d ± 2 d.
Development of adventitious roots in potato microplants (Solanum tuberosum L.) cv. Citlali, inoculated with PSB strain A3 and cultured for 28 d ± 2 d.

Potato microplants (Solanum tuberosum L_) cv_ Citlali grown for 28 ± 2 d in vitro in presence/absence of PSB strain A3_

TreatmentsStem length (cm)Root length (cm)Fresh weight (g)
Bacterial strain A3 added  10.043 ± 3.252*  2.943 ± 1.634*  0.573 ± 0.296*
Without added bacterial strain A35.6 ± 2.0264.297 ± 3.1360.174 ± 0.161

Growth evaluation of potato microplants cv_ Citlali with different P sources and sucrose concentrations_

Variable
Phosphate (A)Sucrose g/l (B)Interaction
KH2PO4 (T0, T1)Ca3(PO4)2 (T4, T5)  10 (T1, T5)  30 (T0, T4)AB
1*6.081 ± 0.154¤b6.801 ± 0.155a6.122 ± 0.153b6.760 ± 0.156aNo
215.099 ± 0.640NS16.180 ± 0.636NS14.335 ± 0.632b16.944 ± 0.644aNo
30.279 ± 0.093NS0.334 ± 0.094NS0.240 ± 0.0195b0.373 ± 0.0198aPresent
DOI: https://doi.org/10.33073/pjm-2020-039 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 357 - 365
Submitted on: Jun 4, 2020
Accepted on: Aug 15, 2020
Published on: Sep 8, 2020
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
sp.,
phosphorus soluble,
Pikovskaya agar,
potato rhizosphere,
plant growth promoting rhizobacteria
Related subjects:
Life sciences,
Microbiology and virology

© 2020 GUSTAVO YAÑEZ-OCAMPO, MARTHA E. MORA-HERRERA, ARNOLDO WONG-VILLARREAL, DENISSE M. DE LA PAZ-OSORIO, NADIA DE LA PORTILLA-LÓPEZ, JORGE LUGO, ROCIO VACA-PAULÍN, PEDRO DEL ÁGUILA, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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