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Lactobacillus fermentum JX306 Restrain D-galactose-induced Oxidative Stress of Mice through its Antioxidant Activity Cover

Lactobacillus fermentum JX306 Restrain D-galactose-induced Oxidative Stress of Mice through its Antioxidant Activity

Open Access
|Jun 2020

Figures & Tables

Fig. 1.

Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the liver of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the liver of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 2.

Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the kidney of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the kidney of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 3.

Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities and MDA concentration in the serum of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities and MDA concentration in the serum of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 4.

Effect of L. fermentum JX306 on the expression of the genes encoding for peroxiredoxin-1 (Prdx1), glutathione peroxidase (Gsr), glutathione peroxidase (Gpx), and thioredoxin reductase (TR3) in the liver of D-galactose induced aging mice. (A) Thioredoxin reductase mRNA; (B) Peroxiredoxin1 mRNA; (C) Glutathione reductase mRNA; (D) Glutathione peroxidase mRNA. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on the expression of the genes encoding for peroxiredoxin-1 (Prdx1), glutathione peroxidase (Gsr), glutathione peroxidase (Gpx), and thioredoxin reductase (TR3) in the liver of D-galactose induced aging mice. (A) Thioredoxin reductase mRNA; (B) Peroxiredoxin1 mRNA; (C) Glutathione reductase mRNA; (D) Glutathione peroxidase mRNA. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 5.

Effects of L. fermentum JX306 treatment on organic damages in the liver (A), and the kidney (B) of D-galactose induced aging mice.
Effects of L. fermentum JX306 treatment on organic damages in the liver (A), and the kidney (B) of D-galactose induced aging mice.

The survival rate (%) of LAB in simulated bile, gastric fluid, and intestinal fluid_

StrainsSimulated bileSimulated gastric fluidSimulated intestinal fluid
L. fermentum JX30678.28 ± 0.18 a 53.05 ± 1.75 a 42.07 ± 6.52 a
L. fermentum GZ39474.61 ± 4.67 a 30.57 ± 3.68 b 27.26 ± 4.95 b
L. plantarum SC3465.09 ± 6.33 b 5.34 ± 1.92 c 12.23 ± 1.85 e
L. splantarum GZ32874.48 ± 1.79 a 1.51 ± 0.22 e 9.75 ± 0.45 f
Pediococcus pentosaceus GZ43022.99 ± 3.12 d 3.62 ± 1.11 d 15.26 ± 2.34 d
Leuconostoc mesenteroides YN29533.89 ± 0.96 c 5.34 ± 0.79 c 17.28 ± 0.56 c

Groups of experimental animals_

GroupNumberExperimental treatments
Oral administrationIntraperitoneal injection
NC10Normal saline (0.85%, 20 ml/kg BW)Normal saline (0.85%, 20 ml/kg BW)
MC10Normal saline (0.85%, 20 ml/kg BW)D-galactose (30 g/l, 200 mg/kg BW)
PC10Vitamin C (30 g/l, 200 mg/kg BW)D-galactose (30 g/l, 200 mg/kg BW)
LD10 L. fermentum JX306 (108 CFU/day)D-galactose (30 g/l, 200 mg/kg BW)
MD10 L. fermentum JX306 (109 CFU/day)D-galactose (30 g/l, 200 mg/kg BW)
HD10 L. fermentum JX306 (1010 CFU/day)D-galactose (30 g/l, 200 mg/kg BW)

The selected LAB strains with high antioxidant activity_

StrainsDPPH scavenging rate (%)Hydrogen radicals scavenging rate (%)Inhibition rate of lipid peroxidation (%)
L. fermentum JX30637.29 ± 1.75 a 37.90 ± 0.29 a 28.14 ± 2.97 a
L. fermentum GZ39434.92 ± 3.57 a 35.02 ± 1.70 b 23.89 ± 1.60 b
L. plantarum SC3423.09 ± 4.00 c 34.50 ± 1.44 b 12.69 ± 0.23 d
L. plantarum GZ32827.83 ± 2.25 b 29.21 ± 1.60 c 20.85 ± 2.07 bc
Pediococcus pentosaceus GZ43016.22 ± 0.89 d 23.32 ± 1.62 d 18.01 ± 2.09 c
Leuconostoc mesenteroides YN29515.26 ± 0.67 d 34.31 ± 1.18 b 14.43 ± 1.29 d
DOI: https://doi.org/10.33073/pjm-2020-024 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 205 - 215
Submitted on: Feb 14, 2020
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Accepted on: Apr 21, 2020
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Published on: Jun 4, 2020
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2020 DI ZHANG, CHUANG LI, RUIRUI SHI, FENGCHUN ZHAO, ZHENGYOU YANG, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.