Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry

JUSTYNA D. KOWALSKA; ADRIANA NOWAK; KATARZYNA ŚLIŻEWSKA; MAŁGORZATA STAŃCZYK; MAGDALENA ŁUKASIAK; JAROSŁAW DASTYCH

doi:10.33073/pjm-2020-001

Figures & Tables

The survival of lactic acid bacteria at 1% and 2% bile salts.

Adherence of Salmonella sp. strains to adenocarcinoma cells Caco-2 in the presence of lactic acid bacteria presented as stimulation/inhibition rate (%).

Cytotoxic effect of probiotics’ supernatants on Caco-2 cells in the range of tested dilutions presented as % of control.

Total antagonistic activity index (IAA) of lactic acid bacteria.

Antagonistic activity of Lactobacillus sp_ strains against Salmonella sp_ (type of inhibition: +++ strong; ++ moderate; + weak; − no inhibition)_

Salmonella sp. strain	Year of Salmonella acquisition	L. rhamnosus LOCK 1131	L. casei LOCK 1132	L. paracasei LOCK 1133
S. Typhimurium ATCC 13311		+++	+++	+++
S. Typhimurium ATCC 14028		+++	+++	+++
S. Enteritidis ATCC 13076		+++	+++	+++
S. Enteritidis 1067/S/09 K-2	2009	+ ^a	+ ^a	+++ ^b
S. Enteritidis 848/S/09	2009	++ ^a	+ ^b	++ ^a
S. Enteritidis Ferma ‘K’ K-9	2010	+ ^a	+ ^a	++ ^b
S. Enteritidis Woź K-7	2010	+ ^a	++ ^a	++ ^b
S. Enteritidis Ferma ‘K’ K-10	2010	++ ^a	+ ^b	++ ^a
S. Enteritidis Ferma ‘K’ K-11	2010	++ ^a	+ ^b	++ ^a
S. Enteritidis Ferma ‘K’ K-12	2010	+ ^a	+ ^b	+ ^a
S. Enteritidis Ferma ‘K’ K-3	2010	+ ^a	++ ^b	+ ^a
S. Enteritidis Ferma ‘K’ K-4	2010	+ ^a	++ ^b	+ ^a
S. Enteritidis Ferma ‘K’ K-6	2010	+ ^a	+ ^b	+ ^a
S. Enteritidis Ferma ‘K’ K-14	2010	+ ^b	+ ^a	++ ^a
S. Enteritidis Ferma ‘K’ K-18	2010	++ ^a	+ ^ab	+ ^b
S. Enteritidis Pac K-piętro	2010	+ ^ab	+ ^a	+ ^b
S. Enteritidis Woź K-6	2010	+	+	+
S. Enteritidis Woź K-9	2010	++ ^a	+ ^ab	+ ^b
S. Enteritidis 64/S/10	2010	+	+	+
S. Enteritidis 249	2010	+	+	+
S. Enteritidis 517/S/09	2009	++ ^a	+ ^ab	+ ^b
S. Enteritidis 571/S/09	2009	+ ^a	+ ^b	++ ^c
S. Enteritidis 833/S/09	2009	+	+	++
S. Enteritidis 838/S/09	2009	++ ^b	+ ^a	+ ^a
S. Enteritidis 838/S/09 B	2009	+	+	++
S. Enteritidis 847/S/09	2009	+ ^a	+ ^a	− ^b
S. Enteritidis 865/S/09	2009	+	+	+
S. Enteritidis 866/S/09	2009	+ ^a	− ^b	+ ^a
S. Enteritidis 945/S/09	2009	++ ^b	+ ^a	− ^a
S. Enteritidis 975/S/09	2009	+	+	+
S. Enteritidis 1013/S/09 K-4	2009	+ ^a	++ ^b	+ ^a
S. Enteritidis 1014/S/09 K-1	2009	+ ^b	++ ^a	+ ^a
S. Enteritidis 1021/S/09	2009	++ ^b	+ ^a	+ ^a
S. Enteritidis 1022/S/09 K-9	2009	++ ^a	++ ^a	+ ^b
S. Enteritidis 1542/S/09 NWJ	2009	+ ^ab	+ ^a	+ ^b
S. Enteritidis 1044/S/09 K-2	2009	+ ^a	− ^b	+ ^a
S. Enteritidis 1445/S/09 K-46	2009	+	+	+
S. Enteritidis 1446/S/09 K-31	2009	++ ^b	+ ^a	+ ^a
S. Enteritidis 1465/S/09 MEK	2009	+ ^a	− ^b	+ ^a
S. Enteritidis 1515/S/09	2009	+ ^a	+ ^ab	+ ^b
S. Enteritidis 1535/S/09 NWJ	2009	+ ^b	+ ^a	+ ^a
S. Enteritidis 1714/09	2009	+	+	+
S. Enteritidis 2050/S/09	2009	+ ^a	+ ^ab	+ ^b
S. Enteritidis 1085/S/09 MEK	2009	++ ^a	++ ^b	+ ^c
S. Enteritidis 1231/S/09	2009	+	+	+
S. Enteritidis 1206/S/09 MEK	2009	++ ^a	+ ^b	− ^c
S. Enteritidis 1171/S/09	2009	a	++ ^b	+ ^c
S. Enteritidis 1257/S/09 K7	2009	++ ^a	++ ^b	+ ^c
S. Enteritidis 1250/S/09	2009	+ ^a	++ ^b	+ ^a
S. Enteritidis 1143/S/09	2009	+ ^a	++ ^b	++ ^ab
S. Enteritidis 1422/S/09 MEK	2009	+ ^a	++ ^b	+ ^c
S. Enteritidis 1106/S/09 K-1	2009	+ ^a	+ ^a	++ ^b
S. Enteritidis 1061/S/09/K-5	2009	++ ^b	++ ^a	++ ^a
S. Enteritidis 1067/S/09 K-3	2009	+	++	+
S. Enteritidis 1573/S/09 NWJ	2009	++ ^a	++ ^b	++ ^c
S. Enteritidis 1192/S/09 K-8	2009	++ ^ab	++ ^a	+ ^b
S. Enteritidis 1047/S/09 K-1	2009	++ ^a	++ ^a	+ ^b
S. Enteritidis 1545/S/09 MEK	2009	+ ^ab	++ ^a	+ ^b
S. Enteritidis 1048/S/09 K-8	2009	+	+	+
S. Enteritidis 1572/S/09 NWJ	2009	++ ^ab	++ ^a	++ ^b
S. Enteritidis 002PP2014	2014	+ ^a	++ ^ab	++ ^b
S. Enteritidis 004PP2014	2014	+ ^a	++ ^b	++ ^b
S. Enteritidis 005PP2014	2014	++ ^a	+ ^b	+ ^c
S. Enteritidis 001PP2014	2014	++	++	++
S. Enteritidis 006PP2014	2014	+	+	+
S. Enteritidis 007PP2014	2014	++ ^a	+ ^b	++ ^a
S. Enteritidis 2149/09	2009	++ ^a	+ ^b	++ ^a
S. Enteritidis 2619/S/10	2010	+ ^b	+ ^a	+ ^a
S. Enteritidis 003PP2014	2014	++ ^a	+ ^a	++ ^b
S. Enteritidis 008PP2014	2014	+ ^b	++ ^a	++ ^a
S. Enteritidis 009PP2014	2014	+ ^a	+ ^b	+ ^a
S. Enteritidis 010PP2014	2014	+ ^a	+ ^b	+ ^a
S. Enteritidis 12617/2/S	2014	+ ^a	+ ^a	++ ^b
S. Enteritidis 12633	2014	+	+	+
S. Enteritidis 12961/1	2014	+ ^b	++ ^a	++ ^a
S. Enteritidis 13001	2014	+ ^a	++ ^ab	++ ^b
S. Enteritidis 011PP2014	2014	++ ^a	++ ^b	++ ^ab
S. Enteritidis 012PP2014	2014	+ ^a	+ ^a	++ ^b
S. Enteritidis 014PP2014	2014	+ ^a	+ ^a	++ ^b
S. Enteritidis 015PP2014	2014	+ ^a	+ ^a	++ ^b
S. Enteritidis 016PP2015	2015	+ ^a	+ ^a	++ ^b
S. Enteritidis 017PP2015	2015	++ ^a	+ ^b	+ ^ab
S. Enteritidis 018PP2015	2015	++	++	++
S. Enteritidis 019PP2015	2015	++ ^a	+ ^b	+ ^c
S. Enteritidis 020PP2016	2016	+	+	+
S. Enteritidis 021PP2016	2016	++	++	++
S. Enteritidis 022PP2016	2016	+ ^a	+ ^b	++ ^a
S. Enteritidis 023PP2016	2016	+	++	+
S. Enteritidis 024PP2016	2016	+ ^a	+ ^b	++ ^c
S. Enteritidis 025PP2016	2016	+	+	+
S. Enteritidis 026PP2016	2016	++ ^a	++ ^ab	+ ^b
S. Enteritidis 027PP2016	2016	++ ^a	+ ^b	+ ^c
S. Enteritidis 028PP2016	2016	+ ^a	− ^b	+ ^c
S. Enteritidis 029PP2016	2016	+ ^a	+ ^b	+ ^a
S. Enteritidis 030PP2016	2016	+ ^a	- ^b	+ ^a
S. Enteritidis 031PP2016	2016	+	+	+
S. Enteritidis 032PP2016	2016	++ ^a	++ ^b	++ ^c
S. Enteritidis 033PP2016	2016	++	++	++
S. Enteritidis 034PP2016	2016	+	+	+
S. Enteritidis 035PP2016	2016	++ ^a	++ ^b	+ ^c
S. Enteritidis 036PP2016	2016	++	++	++
S. Enteritidis 037PP2016	2016	+ ^a	++ ^b	+ ^a
S. Enteritidis 038PP2016	2016	++ ^a	+ ^b	++ ^a
S. Enteritidis 039PP2016	2016	++	++	+
S. Enteritidis 040PP2016	2016	++ ^a	++ ^b	++ ^ab
S. Enteritidis 041PP2016	2016	++ ^b	++ ^a	++ ^a
S. Enteritidis 042PP2016	2016	++	++	++
S. Enteritidis 043PP2016	2016	+ ^a	+ ^a	++ ^b
S. Enteritidis 044PP2016	2016	++ ^a	+++ ^b	++ ^a
S. Enteritidis 045PP2016	2016	++	+++	++
S. Enteritidis 046PP2016	2016	+	++	+
S. Enteritidis 047PP2016	2016	++ ^a	++ ^b	++ ^ab
S. Enteritidis 048PP2016	2016	+ ^b	+ ^a	+ ^a
S. Enteritidis 049PP2016	2016	++ ^a	+++ ^b	+ ^c
S. Enteritidis 050PP2016	2016	++ ^a	+ ^a	+ ^b
S. Enteritidis 051PP2016	2016	+++	+++	+++
S. Enteritidis 53A	2013	++ ^a	+ ^b	++ ^a
S. Enteritidis Korczak 28A	2013	++ ^a	+ ^b	++ ^a
S. Enteritidis Korczak 32A	2013	+	+	+
S. Enteritidis Korczak 40A	2013	++ ^a	+ ^b	+ ^c
S. Enteritidis Korczak 41A	2013	++ ^a	+ ^b	++ ^a
S. Enteritidis 4050/2014 D09	2014	++ ^a	++ ^b	++ ^ab
S. Enteritidis 6537/1/S/14 D09	2014	+ ^a	+ ^b	+ ^ab
S. Enteritidis 6670/1/S/14 D09	2014	+ ^a	+ ^a	+ ^b
S. Enteritidis 7660/1/D03	2014	+ ^a	+ ^b	+ ^c
S. Enteritidis 65/S/10	2010	+ ^a	+ ^ab	+ ^b
S. Enteritidis 1KSFA	2013	++ ^a	+ ^ab	+ ^b

Probiotic properties of Lactobacillus strains_

	Autoaggregation	Coaggregation			Coefficient of adherence to polystyrene	Adherence to Caco-2 cells
		S. Ent. 13076	S. Typ. 13311	S. Typ. 14028		Init. No.	After 2 h	Adherence index
		S. Ent. 13076	S. Typ. 13311	S. Typ. 14028		[log CFU/ml]		Adherence index
L. rhamnosus LOCK 1131	96.1% ± 1.2%	14.8% ± 0.01%	84.2% ± 0.01%	51.8% ± 0.03%	1.18 ± 0.02	8.5	7.6 ± 1.1	89.8%
L. casei LOCK 1132	98.2% ± 3.6%	26.1% ± 0.01%	58.5% ± 0.01%	−11.1%± 0.01%	1.22 ± 0.02	8.5	8.3 ± 0.5	98.0%
L. paracasei LOCK 1133	93.5% ± 0.9%	41.8% ± 3.4%	17.8% ± 3.3%	43.7% ± 2.7%	1.20 ± 0.07	8.0	6.3 ± 0.1	78.9%

Antibiotic resistance of Lactobacillus strains_

	Antibiotic inhibition zone diameter[mm ± SD]
	PEN	KAN	TET	ERY	DOX	AMX
L. rhamnosus LOCK 1131	27 ± 2.5(S)	0(R)	34 ± 1.0(S)	33 ± 2.5(S)	32 ± 2.0(S)	21 ± 2.1(R)
L. casei LOCK 1132	28 ± 2.5(S)	0(R)	30 ± 0.6(S)	34 ± 1.5(S)	33 ± 1.0(S)	24 ± 0.6(IR)
L. paracasei LOCK 1133	27 ± 6.4(S)	0(R)	25 ± 1.5(S)	25 ± 0.0(S)	30 ± 0.6(S)	21 ± 1.0(R)

Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry

Figures & Tables

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Antagonistic activity of Lactobacillus sp_ strains against Salmonella sp_ (type of inhibition: +++ strong; ++ moderate; + weak; − no inhibition)_

Probiotic properties of Lactobacillus strains_

Antibiotic resistance of Lactobacillus strains_

Paradigm

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