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Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry Cover

Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry

Polish Journal of Microbiology
Volume 69 (2020): Issue 1 (March 2020)
By: ,  ,  ,  ,   and    
Open Access
|Jan 2020
Figures & tables

Figures & Tables

Fig. 1.

The survival of lactic acid bacteria at 1% and 2% bile salts.

Fig. 2.

The resistance of LAB to low pH.

Table I

Antibiotic resistance of Lactobacillus strains.

Antibiotic inhibition zone diameter
[mm ± SD]
PENKANTETERYDOXAMX
L. rhamnosus LOCK 113127 ± 2.5
(S)		0
(R)		34 ± 1.0
(S)		33 ± 2.5
(S)		32 ± 2.0
(S)		21 ± 2.1
(R)
L. casei LOCK 113228 ± 2.5
(S)		0
(R)		30 ± 0.6
(S)		34 ± 1.5
(S)		33 ± 1.0
(S)		24 ± 0.6
(IR)
L. paracasei LOCK 113327 ± 6.4
(S)		0
(R)		25 ± 1.5
(S)		25 ± 0.0
(S)		30 ± 0.6
(S)		21 ± 1.0
(R)

1 S: susceptible; R: resistant; IR: intermediate resistant

2 PEN: Penicillin G (1 unit); KAN: Kanamycin (30 μg); TET: Tetracycline (30 μg);

3 ERY: Erythromycin (30 μg); DOX: Doxycycline (30 μg); AMX: Amoxicillin (25 μg)

Table II

Probiotic properties of Lactobacillus strains.

AutoaggregationCoaggregationCoefficient of adherence to polystyreneAdherence to Caco-2 cells
S. Ent. 13076S. Typ. 13311S. Typ. 14028Init. No.After 2 hAdherence index
[log CFU/ml]
L. rhamnosus LOCK 113196.1% ± 1.2%14.8% ± 0.01%84.2% ± 0.01%51.8% ± 0.03%1.18 ± 0.028.57.6 ± 1.189.8%
L. casei LOCK 113298.2% ± 3.6%26.1% ± 0.01%58.5% ± 0.01%−11.1%± 0.01%1.22 ± 0.028.58.3 ± 0.598.0%
L. paracasei LOCK 113393.5% ± 0.9%41.8% ± 3.4%17.8% ± 3.3%43.7% ± 2.7%1.20 ± 0.078.06.3 ± 0.178.9%
Fig. 3.

Adherence of Salmonella sp. strains to adenocarcinoma cells Caco-2 in the presence of lactic acid bacteria presented as stimulation/inhibition rate (%).

Fig. 4.

Cytotoxic effect of probiotics’ supernatants on Caco-2 cells in the range of tested dilutions presented as % of control.

Table III

Antagonistic activity of Lactobacillus sp. strains against Salmonella sp. (type of inhibition: +++ strong; ++ moderate; + weak; − no inhibition).

Salmonella sp. strainYear of Salmonella acquisition L. rhamnosus LOCK 1131 L. casei LOCK 1132 L. paracasei LOCK 1133
S. Typhimurium ATCC 13311+++++++++
S. Typhimurium ATCC 14028+++++++++
S. Enteritidis ATCC 13076+++++++++
S. Enteritidis 1067/S/09 K-22009+ a + a +++ b
S. Enteritidis 848/S/092009++ a + b ++ a
S. Enteritidis Ferma ‘K’ K-92010+ a + a ++ b
S. Enteritidis Woź K-72010+ a ++ a ++ b
S. Enteritidis Ferma ‘K’ K-102010++ a + b ++ a
S. Enteritidis Ferma ‘K’ K-112010++ a + b ++ a
S. Enteritidis Ferma ‘K’ K-122010+ a + b + a
S. Enteritidis Ferma ‘K’ K-32010+ a ++ b + a
S. Enteritidis Ferma ‘K’ K-42010+ a ++ b + a
S. Enteritidis Ferma ‘K’ K-62010+ a + b + a
S. Enteritidis Ferma ‘K’ K-142010+ b + a ++ a
S. Enteritidis Ferma ‘K’ K-182010++ a + ab + b
S. Enteritidis Pac K-piętro2010+ ab + a + b
S. Enteritidis Woź K-62010+++
S. Enteritidis Woź K-92010++ a + ab + b
S. Enteritidis 64/S/102010+++
S. Enteritidis 2492010+++
S. Enteritidis 517/S/092009++ a + ab + b
S. Enteritidis 571/S/092009+ a + b ++ c
S. Enteritidis 833/S/092009++++
S. Enteritidis 838/S/092009++ b + a + a
S. Enteritidis 838/S/09 B2009++++
S. Enteritidis 847/S/092009+ a + a − b
S. Enteritidis 865/S/092009+++
S. Enteritidis 866/S/092009+ a − b + a
S. Enteritidis 945/S/092009++ b + a − a
S. Enteritidis 975/S/092009+++
S. Enteritidis 1013/S/09 K-42009+ a ++ b + a
S. Enteritidis 1014/S/09 K-12009+ b ++ a + a
S. Enteritidis 1021/S/092009++ b + a + a
S. Enteritidis 1022/S/09 K-92009++ a ++ a + b
S. Enteritidis 1542/S/09 NWJ2009+ ab + a + b
S. Enteritidis 1044/S/09 K-22009+ a − b + a
S. Enteritidis 1445/S/09 K-462009+++
S. Enteritidis 1446/S/09 K-312009++ b + a + a
S. Enteritidis 1465/S/09 MEK2009+ a − b + a
S. Enteritidis 1515/S/092009+ a + ab + b
S. Enteritidis 1535/S/09 NWJ2009+ b + a + a
S. Enteritidis 1714/092009+++
S. Enteritidis 2050/S/092009+ a + ab + b
S. Enteritidis 1085/S/09 MEK2009++ a ++ b + c
S. Enteritidis 1231/S/092009+++
S. Enteritidis 1206/S/09 MEK2009++ a + b − c
S. Enteritidis 1171/S/092009a++ b + c
S. Enteritidis 1257/S/09 K72009++ a ++ b + c
S. Enteritidis 1250/S/092009+ a ++ b + a
S. Enteritidis 1143/S/092009+ a ++ b ++ ab
S. Enteritidis 1422/S/09 MEK2009+ a ++ b + c
S. Enteritidis 1106/S/09 K-12009+ a + a ++ b
S. Enteritidis 1061/S/09/K-52009++ b ++ a ++ a
S. Enteritidis 1067/S/09 K-32009++++
S. Enteritidis 1573/S/09 NWJ2009++ a ++ b ++ c
S. Enteritidis 1192/S/09 K-82009++ ab ++ a + b
S. Enteritidis 1047/S/09 K-12009++ a ++ a + b
S. Enteritidis 1545/S/09 MEK2009+ ab ++ a + b
S. Enteritidis 1048/S/09 K-82009+++
S. Enteritidis 1572/S/09 NWJ2009++ ab ++ a ++ b
S. Enteritidis 002PP20142014+ a ++ ab ++ b
S. Enteritidis 004PP20142014+ a ++ b ++ b
S. Enteritidis 005PP20142014++ a + b + c
S. Enteritidis 001PP20142014++++++
S. Enteritidis 006PP20142014+++
S. Enteritidis 007PP20142014++ a + b ++ a
S. Enteritidis 2149/092009++ a + b ++ a
S. Enteritidis 2619/S/102010+ b + a + a
S. Enteritidis 003PP20142014++ a + a ++ b
S. Enteritidis 008PP20142014+ b ++ a ++ a
S. Enteritidis 009PP20142014+ a + b + a
S. Enteritidis 010PP20142014+ a + b + a
S. Enteritidis 12617/2/S2014+ a + a ++ b
S. Enteritidis 126332014+++
S. Enteritidis 12961/12014+ b ++ a ++ a
S. Enteritidis 130012014+ a ++ ab ++ b
S. Enteritidis 011PP20142014++ a ++ b ++ ab
S. Enteritidis 012PP20142014+ a + a ++ b
S. Enteritidis 014PP20142014+ a + a ++ b
S. Enteritidis 015PP20142014+ a + a ++ b
S. Enteritidis 016PP20152015+ a + a ++ b
S. Enteritidis 017PP20152015++ a + b + ab
S. Enteritidis 018PP20152015++++++
S. Enteritidis 019PP20152015++ a + b + c
S. Enteritidis 020PP20162016+++
S. Enteritidis 021PP20162016++++++
S. Enteritidis 022PP20162016+ a + b ++ a
S. Enteritidis 023PP20162016++++
S. Enteritidis 024PP20162016+ a + b ++ c
S. Enteritidis 025PP20162016+++
S. Enteritidis 026PP20162016++ a ++ ab + b
S. Enteritidis 027PP20162016++ a + b + c
S. Enteritidis 028PP20162016+ a − b + c
S. Enteritidis 029PP20162016+ a + b + a
S. Enteritidis 030PP20162016+ a - b + a
S. Enteritidis 031PP20162016+++
S. Enteritidis 032PP20162016++ a ++ b ++ c
S. Enteritidis 033PP20162016++++++
S. Enteritidis 034PP20162016+++
S. Enteritidis 035PP20162016++ a ++ b + c
S. Enteritidis 036PP20162016++++++
S. Enteritidis 037PP20162016+ a ++ b + a
S. Enteritidis 038PP20162016++ a + b ++ a
S. Enteritidis 039PP20162016+++++
S. Enteritidis 040PP20162016++ a ++ b ++ ab
S. Enteritidis 041PP20162016++ b ++ a ++ a
S. Enteritidis 042PP20162016++++++
S. Enteritidis 043PP20162016+ a + a ++ b
S. Enteritidis 044PP20162016++ a +++ b ++ a
S. Enteritidis 045PP20162016+++++++
S. Enteritidis 046PP20162016++++
S. Enteritidis 047PP20162016++ a ++ b ++ ab
S. Enteritidis 048PP20162016+ b + a + a
S. Enteritidis 049PP20162016++ a +++ b + c
S. Enteritidis 050PP20162016++ a + a + b
S. Enteritidis 051PP20162016+++++++++
S. Enteritidis 53A2013++ a + b ++ a
S. Enteritidis Korczak 28A2013++ a + b ++ a
S. Enteritidis Korczak 32A2013+++
S. Enteritidis Korczak 40A2013++ a + b + c
S. Enteritidis Korczak 41A2013++ a + b ++ a
S. Enteritidis 4050/2014 D092014++ a ++ b ++ ab
S. Enteritidis 6537/1/S/14 D092014+ a + b + ab
S. Enteritidis 6670/1/S/14 D092014+ a + a + b
S. Enteritidis 7660/1/D032014+ a + b + c
S. Enteritidis 65/S/102010+ a + ab + b
S. Enteritidis 1KSFA2013++ a + ab + b

4abc Different superscripts in the same row denote statistically significant differences in antagonistic activity of different Lactobacillus strains (p < 0.05).

Fig. 5.

Total antagonistic activity index (IAA) of lactic acid bacteria.

DOI: https://doi.org/10.33073/pjm-2020-001 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 5 - 18
Submitted on: Oct 3, 2019
Accepted on: Dec 2, 2019
Published on: Jan 28, 2020
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Lactobacillus sp.,
Salmonella sp.,
antimicrobial activity,
cytotoxicity,
poultry,
probiotic potential
Related subjects:
Life sciences,
Microbiology and virology

© 2020 JUSTYNA D. KOWALSKA, ADRIANA NOWAK, KATARZYNA ŚLIŻEWSKA, MAŁGORZATA STAŃCZYK, MAGDALENA ŁUKASIAK, JAROSŁAW DASTYCH, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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