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Structural and biomedical evaluation of Capsicum annuum-assisted green synthesis of CuFe2O4 nanoparticles Cover

Structural and biomedical evaluation of Capsicum annuum-assisted green synthesis of CuFe2O4 nanoparticles

Open Access
|Jul 2026

References

  1. Sheena, T.V., Jyothish, B., Jacob, J., Preparation, characterization, and in vitro evaluation of the anticancer activity of Ce3+ doped CuFe2O4 spinel nanoparticles in MCF-7 cell lines, Chem. Phys. Impact., 2024; 8: 100423. 10.1016/j.chphi.2023.100423
  2. Abu-Elsaad, N.I., Mazen, S.A., Kumar, E.R., Structural, vibrational and magnetic properties of heat treated CuFe2O4 nanoparticles prepared by two different synthesis routes, Ceram. Int., 2024; 50(2 Part B): 3693–3700. 10.1016/j.ceramint.2023.11.121
  3. Dhyani, R., Srivastava, R.C., Dixit, G., Study of magnetic and temperature-dependent dielectric properties of Co-CuFe2O4 nanoferrites, J. Electron. Mater., 2022; 51(10): 5492–5507. 10.1007/s11664-022-09831-0
  4. Kombaiah, K., Vijaya, J.J., Kennedy, L.J., Bououdina, M., Al-Najar, B., Conventional and microwave combustion synthesis of optomagnetic CuFe2O4 nanoparticles for hyperthermia studies, J. Phys. Chem. Solids., 2018; 115: 162–171. 10.1016/j.jpcs.2017.12.024
  5. Ramaprasad, T., Kumar, R.J., Naresh, U., Prakash, M., Kothandan, D., Babu Naidu, K.C., Effect of pH value on structural and magnetic properties of CuFe2O4 nanoparticles synthesized by low temperature hydrothermal technique, Mater. Res. Express., 2018; 5(9): 095025. 10.1088/2053-1591/aad860
  6. Fotukian, S.M., Barati, A., Soleymani, M., Alizadeh, A.M., Solvothermal synthesis of CuFe2O4 and Fe3O4 nanoparticles with high heating efficiency for magnetic hyperthermia application, J. Alloys Compd., 2020; 816: 152548. 10.1016/j.jallcom.2019.152548
  7. Abdussalam-mohammed, W., Qasem, A., Errayes, A.O., Green chemistry: Principles, applications, and disadvantages, Chem. Methodol., 2020; 4(4): 408–423. 10.33945/sami/chemm.2020.4.4
  8. Soltys, L., Olkhovyy, O., Tatarchuk, T., Naushad, M., Green synthesis of metal and metal oxide nanoparticles: Principles of green chemistry and raw materials, Magnetochemistry, 2021; 7(11): 145. 10.3390/magnetochemistry7110145
  9. Jabbar, R., Green synthesis of cobalt ferrite nanoparticles: From fundamentals to advanced applications, Next Mater., 2025; 9: 101327. 10.1016/j.nxmate.2025.101327
  10. Singh, P., Kim, Y.J., Zhang, D., Yang, D.C., Biological synthesis of nanoparticles from plants and microorganisms, Trends Biotechnol., 2016; 34(7): 588–599. 10.1016/j.tibtech.2016.02.006
  11. Meenakshi, G., Manjunath, B.C., Prashantha, S.C., Prashanth, T., Surendra, B.S., Super capacitor, electrochemical measurement and sun light driven photocatalytic applications of CuFe2O4 NPs synthesized from bio-resource extract, Sens. Int., 2023; 4: 100237. 10.1016/j.sintl.2023.100237
  12. Makofane, A., Motaung, D.E., Hintsho-Mbita, N.C., Green synthesis of silver deposited on copper ferrite nanoparticles for the photodegradation of dye and antibiotics, Appl. Surf. Sci. Adv., 2024; 21: 100601. 10.1016/j.apsadv.2024.100601
  13. Jasim, S.A., Patra, I., Opulencia, M.J.C., Hachem, K., Parra, R.M.R., Ansari, M.J., et al., Green synthesis of spinel copper ferrite (CuFe2O4) nanoparticles and their toxicity, Nanotechnol. Rev., 2022; 11(1): 2483–2492. 10.1515/ntrev-2022-0143
  14. Cahyana, A.H., Liandi, A.R., Yulizar, Y., Romdoni, Y., Wendari, T.P., Green synthesis of CuFe2O4 nanoparticles mediated by Morus alba L. leaf extract: Crystal structure, grain morphology, particle size, magnetic and catalytic properties in Mannich reaction, Ceram. Int., 2021; 47(15): 21373–21380. 10.1016/j.ceramint.2021.04.146
  15. Udhaya, P.A., Ahmad, A., Meena, M., Abila Jeba Queen, M., Aravind, M., Velusamy, P., et al., Copper Ferrite nanoparticles synthesised using a novel green synthesis route: Structural development and photocatalytic activity, J. Mol. Struct., 2023; 1277: 134807. 10.1016/j.molstruc.2022.134807
  16. Shotorbani, N.Y., Jamei, R., Heidari, R., Antioxidant activities of two sweet pepper Capsicum annuum L. varieties phenolic extracts and the effects of thermal treatment, Avicenna J. Phytomed., 2013; 3(1): 25–34
  17. Guilherme, R., Aires, A., Rodrigues, N., Peres, A.M., Pereira, J.A., Phenolics and antioxidant activity of green and red sweet peppers from organic and conventional agriculture: A comparative study, Agriculture, 2020; 10(12): 652. 10.3390/agriculture10120652
  18. Al-Qasmi, N., Almughem, F.A., Jarallah, S.J., Almaabadi, A., Efficient green synthesis of (Fe3O4) and (NiFe2O4) nanoparticles using star anise (Illicium verum) extract and their biomedical activity against some cancer cells, Materials (Basel), 2022; 15(14): 4832. 10.3390/ma15144832
  19. Shah, V., Medina-Cruz, D., Vernet-Crua, A., Truong, L.B., Sotelo, E., Mostafavi, E., et al., Pepper-mediated green synthesis of selenium and tellurium nanoparticles with antibacterial and anticancer potential, J. Funct. Biomater, 2023; 14(1): 24. 10.3390/jfb14010024
  20. Khashan, K.S., Badr, B.A., Sulaiman, G.M., Jabir, M.S., Hussain, S.A., Antibacterial activity of Zinc Oxide nanostructured materials synthesis by laser ablation method, J. Phys. Conf. Ser, 2021; 1973(1): 012040. 10.1088/1742-6596/1973/1/012040
  21. Jihad, M.A., Noori, F.T.M., Jabir, M.S., Albukhaty, S., AlMalki, F.A., Alyamani, A.A., Polyethylene glycol functionalized graphene oxide nanoparticles loaded with nigella sativa extract: a smart antibacterial therapeutic drug delivery system, Molecules, 2021; 26(11): 3067. 10.3390/molecules26113067
  22. Mohammed, M.K.A., Mohammad, M.R., Jabir, M.S., Ahmed, D.S., Functionalization, characterization, and antibacterial activity of single wall and multi wall carbon nanotubes, IOP Conf. Ser. Mater. Sci. Eng., 2020; 928(1): 012028. 10.1088/1757-899X/928/1/012028
  23. Al Rugaie, O., Al-Shammari, A.M., Al-Saadi, H., Al-Shammari, S.M., Jabir, M.S., Sulaiman, G.M., Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway, Sci. Rep., 2022; 12(1): 17203. 10.1038/s41598-022-21151-5
  24. Al-Shammari, A.M., Al-Saadi, H., Al-Shammari, S.M., Jabir, M.S., Galangin enhances gold nanoparticles as anti-tumor agents against ovarian cancer cells, AIP Conf. Proc., 2020; 2213(1): 020206. 10.1063/5.0000216
  25. Jawad, M., Öztürk, K., Jabir, M.S. TNF-α loaded on gold nanoparticles as promising drug delivery system against proliferation of breast cancer cells, Mater. Today Proc., 2021; 42(Part 5): 3057–3061. 10.1016/j.matpr.2020.12.829
  26. Mohammed, S.A.A., Al-Azawi, A.M., Jabir, M.S., Al-Shammari, A.M., Sulaiman, G.M., Khan, R.A., Copper oxide nanoparticle‐decorated carbon nanoparticle composite colloidal preparation through laser ablation for antimicrobial and antiproliferative actions against breast cancer cell line, MCF‐7, Biomed. Res. Int., 2022; 2022: 9863616. 10.1155/2022/9863616
  27. Alhujaily, M., Al-Saadi, H., Al-Shammari, S.M., Jabir, M.S., Sulaiman, G.M., AlMalki, F.A., Au/ZnO nanocomposites prepared by laser ablation for enhancement of antibacterial activity and cytotoxic properties against cancer cells, Metals (Basel), 2023; 13(4): 735. 10.3390/met13040735
  28. Carpenter, A.M., van Hoek, M.L., Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction, J. Immunol. Methods, 2024; 529: 113670. 10.1016/j.jim.2024.113670
  29. Hunyek, A., Sirisathitkul, C., Koyvanich, K., Tapioca starch in the sol-gel synthesis of cobalt ferrites with divalent cation substitutions, Karbala Int. J. Mod. Sci., 2022; 8(3): 397–405. 10.33640/2405-609X.3250
  30. Annathurai, S., Chidambaram, S., Baskaran, B., Prasanna Venkatesan, G.K.D., Green synthesis and electrical properties of p-CuO/n-ZnO heterojunction diodes, J. Inorg. Organomet. Polym. Mater., 2019; 29(2): 535–540. 10.1007/s10904-018-1026-1
  31. Pramana, Y.B., Setiawan, B., Prihono, P., Utomo, Y., Subandowo, M., Budipramana, K., A simple synthesis of nickel oxide nanotube using high voltage electrolysis, J. Neutrino, 2021; 13(1): 13–18. 10.18860/neu.v13i1.10224
  32. Hjiri, M., Alonizan, N.H., Althubayti, M.M., Alshammari, S., Besbes, H., Aida, M.S., Correction to: Preparation and photoluminescence of NiFe2O4 nanoparticles, J. Mater. Sci. Mater. Electron., 2020; 31(1): 859–860. 10.1007/s10854-019-02750-7
  33. Mosabberul Haque, M., Islam, M.S., Rahman, M.M., Ahmed, S., Hasan, M., Asaduzzaman, M., Manganese doped copper ferrite nanoparticles: A promising approach for organic dye elimination under light irradiation, Results Chem., 2024; 7: 101509. 10.1016/j.rechem.2024.101509
  34. Gayathri Manju, B., Raji, P., Green synthesis of Nickel–Copper mixed ferrite nanoparticles: Structural, optical, magnetic, electrochemical and antibacterial studies, J. Electron. Mater., 2019; 48(12): 7710–7720. 10.1007/s11664-019-07603-x
  35. Li, S., Shen, Y., Xie, A., Yu, X., Qiu, L., Zhang, L., et al., Green synthesis of silver nanoparticles using Capsicum annuum L. Extract, Green Chem., 2007; 9(8): 852–858. 10.1039/b615357g
  36. Mar, E., Leyva-Porras, C., Saavedra-Leos, M.Z., Toxqui-Terán, A., Pérez-García, C.E., Espinosa-Solis, V., Agro-waste sweet pepper extract-magnetic iron oxide nanoparticles for antioxidant enrichment and sustainable nanopackaging, Polymers (Basel), 2024; 16(4): 564. 10.3390/polym16040564
  37. Muhaymin, A., Ahmad Mohamed, H.E., Hkiri, K., Safdar, A., Kotsedi, L., Maaza, M., Green synthesis of NiFe2O4 nanoparticles using Hyphaene thebaica: A facile route towards magnetic and photocatalytic application, Mater. Today Chem., 2024; 40: 102286. 10.1016/j.mtchem.2024.102286
  38. Adawiah, A., Zulys, A., Fitria, I., Khalil, M., Aziz, I., Khalid, M., Perylene-based metal-organic frameworks-decorated Zinc Ferrite for enhanced photodegradation of malachite green in aqueous system, S. Afr. J. Chem. Eng., 2025; 53: 319–330. 10.1016/j.sajce.2025.05.005
  39. Naghizadeh, A., Mohammadi-Aghdam, S., Mortazavi-Derazkola, S., Novel CoFe2O4@ZnO-CeO2 ternary nanocomposite: Sonochemical green synthesis using Crataegus microphylla extract, characterization and their application in catalytic and antibacterial activities, Bioorg. Chem., 2020; 103: 104194. 10.1016/j.bioorg.2020.104194
  40. Mohammed, A., Alzahran, F, Arshad, J., Al-Buriahi, M.S., Alrowaili, Z.A., Munir, S., Synthesis of graphene-based Ag-doped CuFe2O4 composite for improved photocatalytic activity against industrial effluents, J. Taibah Univ. Sci., 2023; 17(1): 2209676. 10.1080/16583655.2023.2209676
  41. Modhave, S.S., Patil, K.L., Shinde, D.R., Chaskar, M.G., Pawar, R.A., Synthesis and characterization of nanocrystalline rare-earth substituted Copper Ferrites by precursor method and studies its photo-catalytic activity, J. Emerg. Technol. Innov. Res., 2019; 6(5): 266–278
  42. Busharat, M.A., Shahzad, A., Sabir, M., Bilal, M., Khan, M.A., Iqbal, J., et al., Study of cation distribution and photocatalytic activity of nonthermal plasma-modified NiZnFe2O4 magnetic nanocomposites, ACS Omega, 2024; 9(13): 14791–14804. 10.1021/acsomega.3c06883
  43. Abu-Elsaad, N.I., Nawara, A.S., Effect of Cu substitution on magnetic and photocatalytic properties of Mn–ZnFe2O4 nanoparticles, J. Mater. Sci., 2024; 59(10): 4167–4185. 10.1007/s10853-024-09486-8
  44. Deligiannakis, Y., Sotiriou, G.A., Pratsinis, S.E., Antioxidant and antiradical SiO2 nanoparticles covalently functionalized with gallic acid, ACS Appl. Mater. Interfaces, 2012; 4(12): 6609–6617. 10.1021/am3020759
  45. Schwartz, V.B., Thétiot, F., Ritz, S., Eickenscheidt, S., Schäfer, C., Flitsch, S.L., et al., Antibacterial surface coatings from zinc oxide nanoparticles embedded in poly(N-isopropylacrylamide) hydrogel surface layers, Adv. Funct. Mater., 2022; 22(11): 2376–2386. 10.1002/adfm.201102874
  46. Raffi, M., Hussain, F., Oliver, T.M., Dittmar, R.W., Oliver, S.M., Dittmar, K., Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli, Ann. Microbiol., 2010; 60(1): 75–80. 10.1007/s13213-010-0015-6
  47. Wang, J., Zhang, L., Liu, X., Zhao, Y., Wang, H., Chen, Y., Synthesis and potent antibacterial activity of nano-CuFe2O4/MoS2@Ag composite under visible light, Appl. Surf. Sci., 2025; 684: 161908. 10.1016/j.apsusc.2024.161908
  48. Alnehia, A., Hadi, M., Alnahari, H., Al-Sharabi, A., Optical, structural and antibacterial properties of phase heterostructured Fe2O3–CuO–CuFe2O4 nanocomposite, Sci. Rep., 2024; 14(1): 14392. 10.1038/s41598-024-65123-5
  49. Hamid, M.T., Hussein, N.N., Sulaiman, G.M., Mohammed, H.A., Khan, R.A., Antibacterial and antibiofilm properties of silver nanoparticles synthesized using Carthamus tinctorius extract against various multidrug-resistant bacterial strains. Discov, Appl. Sci., 2025; 7(6): 548. 10.1007/s42452-025-06312-0
  50. Ahmed, M.S., Hussein, N.N., Sulaiman, G.M., Mohammed, H.A., Khan, R.A., Enhanced anti-microbial activity of ofloxacin-loaded mesoporous silica nanoparticles against clinical isolates of drug-resistant microbes, J. Ind. Eng. Chem., 2025; 148: 541–559. 10.1016/j.jiec.2024.11.012
  51. Kanagesan, S., Aziz, S.B.A., Hashim, M., Altheraitee, M.S., Jesurani, S., Rao, L.N., et al., Evaluation of antioxidant and cytotoxicity activities of copper ferrite (CuFe2O4) and zinc ferrite (ZnFe2O4) nanoparticles synthesized by sol-gel self-combustion method, Appl. Sci., 2016; 6(9): 184. 10.3390/app6090184
  52. Jasim, A.J., Al-Gawhari, F., Al-Karagoly, H., Sulaiman, G.M., Jabir, M.S., Preliminary trials of the gold nanoparticles conjugated chrysin: An assessment of anti-oxidant, anti-microbial, and in vitro cytotoxic activities of a nanoformulated flavonoid, Nanotechnol. Rev., 2022; 11(1): 2726–2741. 10.1515/ntrev-2022-0169
  53. Shakeel, V., Gul, I.H., John, P., Bhatti, A., Biocompatible gelatin-coated ferrite nanoparticles: A magnetic approach to advanced drug delivery, Saudi Pharm. J., 2024; 32(6): 102066. 10.1016/j.jsps.2024.102066
  54. Tkachenko, A., Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review), Toxicol. Vitr., 2024; 98: 105814. 10.1016/j.tiv.2024.105814
  55. Ergin, İ., Özçelik, S., Yalçın, B., Arda, L., İçin, K., Özçelik, B., Sr-doped CuFe2O4 nanoparticles: Exploring structural, magnetic, and blood compatibility characteristics, Ceram. Int., 2024; 50(18): 33656–33665. 10.1016/j.ceramint.2024.06.123
DOI: https://doi.org/10.2478/msp-2026-0010 | Journal eISSN: 2083-134X | Journal ISSN: 2083-1331
Language: English
Page range: 164 - 179
Submitted on: May 8, 2026
Accepted on: Jun 1, 2026
Published on: Jul 10, 2026
In partnership with: Paradigm Publishing Services

© 2026 Sara H. Shahatha, Rihab Jabbar, Mohammed Hayder Ismail Alluaibi, published by Wroclaw University of Science and Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.