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Testing the effectiveness of microbial biostimulants to increase the resistance of the variety Chardonnay to “Bois noir” phytoplasma Cover

Testing the effectiveness of microbial biostimulants to increase the resistance of the variety Chardonnay to “Bois noir” phytoplasma

Open Access
|Jul 2025

Figures & Tables

Fig. 1:

Precipitation in Mai – August 2023.
Precipitation in Mai – August 2023.

Fig. 2:

Precipitation in Mai – August 2024.
Precipitation in Mai – August 2024.

Fig. 3:

Evaluation of the development of vines with Bois noir symptoms for each variant in 2023.
Evaluation of the development of vines with Bois noir symptoms for each variant in 2023.

Fig. 4:

Evaluation of the development of vines with Bois noir symptoms for each variant in 2024
Evaluation of the development of vines with Bois noir symptoms for each variant in 2024

Fig. 5:

Spatial mapping of plants based on categories
Spatial mapping of plants based on categories

Characteristics of used biostimulants_

NameProducerCharacteristic
NovaFerm® SIRIUSNova Scienta Kft., H-6230, Soltvadkert Kiss Ernő út 75 Hungary
  • Microbial strain: Bacillus licheniformis (min. 1×107 cfu/ml) - max. 5%.

  • pH value: 6.5 – 7.5

  • UV-resistant, good tolerance to low and high temperatures, no risk of resistance, zero safety period.

NovaFerm® ORIONNova Scienta Kft., H-6230, Soltvadkert Kiss Ernő út 75 Hungary
  • Microbial strain: Bacillus thuringiensis spp. kurstaki, Bacillus thuringiensis spp tenebrionis (1×109 cfu/ml), Photorhabdus luminescens (1×109 cfu/ml)

  • pH value: 6.5 – 7.5

  • UV-resistant, good tolerance to low and high temperatures, no risk of resistance, zero safety period.

Microfertile CarbonEkolive, Paderborn, Germany
  • Microbial strain: genus Thiobacillus.

  • pH value: 7–8

Bois noir symptom methodology for evaluating symptoms in the vineyard

Part of the plantThe symptoms
Leaf
  • Leaves are discoloured, edges roll downward, leaf blade becomes brittle.

  • Leaf rolling often results in an angular shape.

  • Spots form on sun-exposed surfaces of the leaves, which enlarge into bands along the veins. These will gradually cover the entire leaf surface.

  • Colour of the spots and bands varies with the cultivar: white-fruited varieties usually have creamy-yellow spots and red-fruited cultivars have reddish spots.

Cluster
  • Flowers dry out and fruit will not set on initially infected plants.

  • Bunches on plants that become infected later in the season will become brown and shrivelled.

  • Fruit will drop very easily with some varieties.

Shoot
  • Canes ripen poorly and unevenly, often showing green and brown sections along the cane.

  • Canes with poorly ripened wood will appear rubbery, often causing a “weeping” appearance.

Assessment of BN symptoms incidence on vines in 2023_ Letters indicate differences between variants in each month according to the test of minimal significant difference at the significance level p<0_05_

Vines categorycontrolMicrofertileNovaFerm OrionNovaFerm SiriusKruskall-Wallis analysis
JulyVines without symptoms472 b467 b428 a427 aSign.
Vines with symptoms62526265n.s.
Vines with poor growth49608877n.s.
Missing vines5 a9 a4 a19 bSign.
AugustVines without symptoms415416408350n.s.
Vines with symptoms1059397137n.s.
Vines with poor growth63707381n.s.
Missing vines5 a9 a4 a19 bsign.
SeptemberVines without symptoms302321349313n.s.
Vines with symptoms225 c222 bc177 a192 abSign.
Vines with poor growth56363264n.s.
Missing vines5 a9 a4 a19 bSign.

Assessment of symptom incidence on plants in 2024_ Letters indicate differences between variants in each month according to the test of minimal significant difference at the significance level p<0_05_

Vines categorycontrolMicrofertileNovaFerm OrionNovaFerm SiriusKruskall-Wallis analysis
JulyVines without symptoms398402388396n.s.
Vines with symptoms1091089373n.s.
Vines with poor growth74699799n.s.
Missing vines791020n.s.
AugustVines without symptoms355381374341n.s.
Vines with symptoms147 b123a125 a149 bSign.
Vines with poor growth79757977n.s.
Missing vines781021n.s.
SeptemberVines without symptoms255 a308 bc329 c270 abSign.
Vines with symptoms286 c237 b215 a252 bSign.
Vines with poor growth40343445n.s.
Missing vines791021n.s.

Comparison of symptom change on plants year on year_

controlMicrofertile CarbonNovaFerm OrionNovaFerm Sirius
From a sick plant to a healthy one16162316
From the healthy plant to a sick one58295161
DOI: https://doi.org/10.2478/mittklbg-2025-0001 | Journal eISSN: 3061-063X | Journal ISSN: 3061-0621
Language: English
Page range: 1 - 14
Submitted on: Jan 7, 2025
Published on: Jul 11, 2025
Published by: High School and Federal Office of Viticulture and Pomology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Pavel Pavloušek, Pavlína Hornychová, Ivana Flajšingerová, published by High School and Federal Office of Viticulture and Pomology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.