Testing the effectiveness of microbial biostimulants to increase the resistance of the variety Chardonnay to “Bois noir” phytoplasma

Name	Producer	Characteristic
NovaFerm^® SIRIUS	Nova Scienta Kft., H-6230, Soltvadkert Kiss Ernő út 75 Hungary	Microbial strain: Bacillus licheniformis (min. 1×10⁷ cfu/ml) - max. 5%. pH value: 6.5 – 7.5 UV-resistant, good tolerance to low and high temperatures, no risk of resistance, zero safety period.
NovaFerm^® ORION	Nova Scienta Kft., H-6230, Soltvadkert Kiss Ernő út 75 Hungary	Microbial strain: Bacillus thuringiensis spp. kurstaki, Bacillus thuringiensis spp tenebrionis (1×10⁹ cfu/ml), Photorhabdus luminescens (1×10⁹ cfu/ml) pH value: 6.5 – 7.5 UV-resistant, good tolerance to low and high temperatures, no risk of resistance, zero safety period.
Microfertile Carbon	Ekolive, Paderborn, Germany	Microbial strain: genus Thiobacillus. pH value: 7–8

Tab. 2:

Bois noir symptom methodology for evaluating symptoms in the vineyard

Part of the plant	The symptoms
Leaf	Leaves are discoloured, edges roll downward, leaf blade becomes brittle. Leaf rolling often results in an angular shape. Spots form on sun-exposed surfaces of the leaves, which enlarge into bands along the veins. These will gradually cover the entire leaf surface. Colour of the spots and bands varies with the cultivar: white-fruited varieties usually have creamy-yellow spots and red-fruited cultivars have reddish spots.
Cluster	Flowers dry out and fruit will not set on initially infected plants. Bunches on plants that become infected later in the season will become brown and shrivelled. Fruit will drop very easily with some varieties.
Shoot	Canes ripen poorly and unevenly, often showing green and brown sections along the cane. Canes with poorly ripened wood will appear rubbery, often causing a “weeping” appearance.

Tab. 3:

Assessment of BN symptoms incidence on vines in 2023. Letters indicate differences between variants in each month according to the test of minimal significant difference at the significance level p<0.05.

	Vines category	control	Microfertile	NovaFerm Orion	NovaFerm Sirius	Kruskall-Wallis analysis
July	Vines without symptoms	472 b	467 b	428 a	427 a	Sign.
	Vines with symptoms	62	52	62	65	n.s.
	Vines with poor growth	49	60	88	77	n.s.
	Missing vines	5 a	9 a	4 a	19 b	Sign.
August	Vines without symptoms	415	416	408	350	n.s.
	Vines with symptoms	105	93	97	137	n.s.
	Vines with poor growth	63	70	73	81	n.s.
	Missing vines	5 a	9 a	4 a	19 b	sign.
September	Vines without symptoms	302	321	349	313	n.s.
	Vines with symptoms	225 c	222 bc	177 a	192 ab	Sign.
	Vines with poor growth	56	36	32	64	n.s.
	Missing vines	5 a	9 a	4 a	19 b	Sign.

Evaluation of the development of vines with Bois noir symptoms for each variant in 2023.

Tab. 4:

Assessment of symptom incidence on plants in 2024. Letters indicate differences between variants in each month according to the test of minimal significant difference at the significance level p<0.05.

	Vines category	control	Microfertile	NovaFerm Orion	NovaFerm Sirius	Kruskall-Wallis analysis
July	Vines without symptoms	398	402	388	396	n.s.
	Vines with symptoms	109	108	93	73	n.s.
	Vines with poor growth	74	69	97	99	n.s.
	Missing vines	7	9	10	20	n.s.
August	Vines without symptoms	355	381	374	341	n.s.
	Vines with symptoms	147 b	123a	125 a	149 b	Sign.
	Vines with poor growth	79	75	79	77	n.s.
	Missing vines	7	8	10	21	n.s.
September	Vines without symptoms	255 a	308 bc	329 c	270 ab	Sign.
	Vines with symptoms	286 c	237 b	215 a	252 b	Sign.
	Vines with poor growth	40	34	34	45	n.s.
	Missing vines	7	9	10	21	n.s.

Evaluation of the development of vines with Bois noir symptoms for each variant in 2024

Spatial mapping of plants based on categories

Tab. 5:

Comparison of symptom change on plants year on year.

	control	Microfertile Carbon	NovaFerm Orion	NovaFerm Sirius
From a sick plant to a healthy one	16	16	23	16
From the healthy plant to a sick one	58	29	51	61

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.2478/mittklbg-2025-0001 | Journal eISSN: 3061-063X (formerly 0007-5922) | Journal ISSN: 3061-0621

Journal RSS Feed

Language: English, German

Page range: 1 - 14

Submitted on: Jan 7, 2025

Published on: Jul 11, 2025

Published by: High School and Federal Office of Viticulture and Pomology

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Related subjects:

© 2025 Pavel Pavloušek, Pavlína Hornychová, Ivana Flajšingerová, published by High School and Federal Office of Viticulture and Pomology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 75 (2025): Issue 1 (March 2025)

Testing the effectiveness of microbial biostimulants to increase the resistance of the variety Chardonnay to “Bois noir” phytoplasma

Figures & Tables

Tab. 1:

Fig. 1:

Fig. 2:

Tab. 2:

Tab. 3:

Fig. 3:

Tab. 4:

Fig. 4:

Fig. 5:

Tab. 5:

Paradigm

My account