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Yolkin promotes differentiation of RAW 264.7 macrophages to the pro-inflammatory phenotype capable of fulfilling efficient phagocytic function Cover

Yolkin promotes differentiation of RAW 264.7 macrophages to the pro-inflammatory phenotype capable of fulfilling efficient phagocytic function

Open Access
|Nov 2026
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References

  1. Ajit J., Chen Q., Ung T., Rosenberger M., Kim, J., Solanki A., Shen J., Aaron P., Kahn E.: β-glucan induced trained immunity enhances antibody levels in a vaccination model in mice. PLoS One 2005, 20, e0323376, doi: 10.1371/journal.pone.0323376.
  2. Alves M.M.M., Arcanjo D.D.R., Carvalho R.C.V. de, Amorim L.V., Santos I.L. dos, Santos R.R.L., Figueiredo K.A., Figueiredo J.F.S., Sobrinho-Júnior E.P.C., Cruz L.P.L. da, Sousa V.C., Santos L.P. dos, Goncalves J.C.R., Carneiro S.M.P., Mendonça I.L. de, Carvalho F.A.A.: Methods of macrophages activation and their modulation for the prospection of new antileishmania drugs: a review. Bioscience J 2021, 37, e37077, doi: 10.14393/BJ-v37n0a2021-53770.
  3. Antal P., Sipka S., Surányi P., Csipo I., Seres T., Maródi L., Szegedi G.: Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake. Ann Hematol 1995, 70, 259265, doi: 10.1007/BF01784045.
  4. Brito R.R.N., Toledo M.S., Labussiere G.M., Dupin T.V., Reis N.F.C., Perez E.C., Xander P.: B-1 cell response in immunity against parasites. Parasitol Res 2019, 118, 1343–1352, doi: 10.1007/s00436-019-06211-2.
  5. Chen J., Liu T., Luo J., Chen Z., Yu Y., Sun H., Tan M., Sun Y., Li S., Li C., Ding Y.: HDAC6/Trim21 aggravates macrophage inflammatory response and titanium-induced osteolysis via GSDMD signaling pathway. J Immunol 2025, 214, 2357–2370, doi: 10.1093/jimmun/vkaf112.
  6. Chen J., Yang Y., Xu Z., Li F., Yang M., Shi F., Lin L., Qin Z.: Characterization of effects of chitooligosaccharide monomer addition on immunomodulatory activity in macrophages. Food Res Int 2023, 163, 112268, doi: 10.1016/j.foodres.2022.112268.
  7. Choi H.J., Park G.H., Choi J.W., Park S.J., Hwang J.H., Lee S.H., Kwon H.-Y., Choi M.Y., Jeong J.B.: Immunostimulatory activity of the aqueous extract from the leaves of Sambucus racemosa subsp. pendula through TLR4-dependent JNK activation in RAW264.7 cells. Biomed Rep 2024, 21, 133, doi: 10.3892/br.2024.1821.
  8. Depierre M., Pompili C., Niedergang F.: Phagocytosis at a glance. J Cell Sci 2025, 38, jcs263833, doi: 10.1242/jcs.263833.
  9. Gambero M., Teixeira D., Butin L., Ishimura M.E., Mariano M., Popi A.F., Longo-Maugéri I.M.: Propionibacterium acnes induces an adjuvant effect in B-1 cells and affects their phagocyte differentiation via a TLR2-mediated mechanism. Immunobiology 2016, 221, 1001–1011, doi: 10.1016/j.imbio.2016.03.003.
  10. Hansen M.B., Nielsen S.E., Berg K.: Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 1989, 119, 203–210, doi: 10.1016/0022-1759(89)90397-9.
  11. Holub M., Cheng C-W., Mott S., Wintermeyer P., van Rooijen N., Gregory S.H.: Neutrophils sequestered in the liver suppress the proinflammatory response of Kupffer cells to systemic bacterial infection. J Immunol 2009, 183, 3309–3316, doi: 10.4049/jimmunol.0803041.
  12. Hsu A.Y., Huang Q., Liu F., Balasubramanian A., Luo H.R.: Neutrophil death-more than meets the eye. Exp Hematol 2025, 150, 104857, doi: 10.1016/j.exphem.2025.104857.
  13. Kashimura M.: Blood defence system – Proposal for a new concept of an immune system against blood borne pathogens comprising the liver, spleen and bone marrow. Scand J Immunol 2024, 99, e13363, doi: 10.1111/sji.13363.
  14. Kazana W., Jakubczyk D., Pacyga-Prus K., Leszczyńska K., Górska S., Siednienko J., Macała J., Piechowiak G., Zabłocka A.: A novel mechanism of macrophage activation by the natural yolkin polypeptide complex from egg yolk. Int J Mol Sci 2022, 23, 3125, doi: 10.3390/ijms23063125.
  15. Kazana W., Mitkiewicz M., Ochnik M., Sochocka M., Zambrowicz A., Piechowiak G, Macała J., Miernikiewicz P., Zabłocka A.: Yolkin isolated from hen 17 egg yolk as a natural immunoregulator, activating innate immune response in BMDM macrophages. Oxid Med Cell Longev 2020, 2020, 5731021, doi: 10.1155/2020/5731021.
  16. Kenzo K., Komine K.-i., Komine Y., Kuroishi T., Kozutsumi T., Kobayashi J., Ohta M., Kitamura H., Kumagai K.: Lactoferrin stimulates a Staphylococcus aureus killing activity of bovine phagocytes in the mammary gland. Microbiol Immunol 2002, 46, 187–194, doi: 10.1111/j.1348-0421.2002.tb02685.x.
  17. Lee J.H., Moon S.H., Kim H.S., Park E., Ahn D.U., Paik H.D.: Immune-enhancing activity of phosvitin by stimulating the production of pro-inflammatory mediator. Poult Sci 2017, 96, 3872–3878, doi: 10.3382/ps/pex205.
  18. Li Q., Zhao T., Mao G., Feng W., Chen Y., Zou T., Yang L., Qian J.-Y.: A Se-enriched Grifola frondosa polysaccharide induces macrophage activation by TLR4-mediated MAPK signaling pathway. Int J Biol Macromol 2023, 238, 124108, doi: 10.1016/j.ijbiomac.2023.124108.
  19. Long L., Li M., Wang M., Liang B., Huang M., Yuan X., Wu X., Guo X., Li S., Liu Z., Liu W., Chen W., Wang W., Lyu Q., Li C.: Activation of mannose receptor C type 1 in macrophages improves renal fibrosis through mediating fibronectin endocytosis. Life Sci 2025, 371, 123593, doi: 10.1016/j.lfs.2025.123593.
  20. Mylvaganam S., Freeman S.A., Grinstein S.: The cytoskeleton in phagocytosis and macropinocytosis. Curr Biol 2021, 31, R619–R632, doi: 10.1016/j.cub.2021.01.036.
  21. Obmińska-Mrukowicz B., Szczypka M., Lis M., Pawlak A., Suszko-Pawłowska A., Sysak A, Zambrowicz A., Burster T., Kocięba M., Artym J., Zaczyńska E., Kochanowska I., Zimecki M.: Effects of yolkin on the immune response of mice and its plausible mechanism of action. Immunol Lett 2020, 220, 21–31, doi: 10.1016/j.imlet.2020.01.003.
  22. Polanowski A., Sosnowska A., Zabłocka A., Janusz M., Trziszka T.: Immunologically active peptides that accompany hen egg yolk immunoglobulin Y: separation and identification. Biol Chem 2013, 394, 879–887, doi: 10.1515/hsz-2012-0337.
  23. Rodrigues K.A.F., Amorim L.V., Dias C.N., Moraes D.F.C., Carneiro S.M.P., Carvalho F.A.A.: Syzygium cumini (L.) Skeels essential oil and its major constituent α-pinene exhibit anti-Leishmania activity through immunomodulation in vitro. J Ethnopharmacol 2015, 160, 32–40, doi: 10.1016/j.jep.2014.11.024.
  24. Salloum G., Bresnick A.R., Backer J.M.: Macropinocytosis: mechanisms and regulation. Biochem J 2023, 480, 335–362, https://doi.org/10.1042/BCJ20210584.
  25. Sandhu K.K., Scott A., Tatler A.L., Belchamber K.B.R., Cox M.J.: Macrophages and the microbiome in chronic obstructive pulmonary disease. Eur Respir Rev 2024, 33, 240053, doi: 10.1183/16000617.0053-2024.
  26. Sigola L.B., Fuentes A.-L., Millis L.M., Vapenik J., Murira A.: Effects of Toll-like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis. Tissue Cell 2016, 48, 389–396, doi: 10.1016/j.tice.2016.04.002.
  27. Souza A.C., Alves M.M.M., Brito L.M., Oliveira L.G.C., Sobrinho-Júnior E.P.C., Costa I.C.G., Freitas S.D.L., Rodrigues K.A.F., Chaves M.H., Arcanjo D.D.R., Carvalho F.A.A.: Platonia insignis Mart., a Brazilian Amazonian plant: the stem barks extract and its main constituent lupeol exert antileishmanial effects involving macrophages activation. Evid-Based Complementary Altern Med 2017, 3126458, doi: 10.1155/2017/3126458.
  28. Tian H., Ling N., Guo C., Gao M., Wang Z., Liu B., Sun Y., Chen Y., Ji C., Li. W.: Immunostimulatory activity of sea buckthorn polysaccharides via TLR2/4-mediated MAPK and NF-κB signaling pathways in vitro and in vivo. Int J Biol Macromol 2024, 283, 137678, doi: 10.1016/j.ijbiomac.2024.137678.
  29. Weeks B.A., Keisler A.S., Myrvik Q.N., Warinner J.E.: Differential uptake of neutral red by macrophages from three species of estuarine fish. Dev Comp Immunol 1987, 11, 117–124, doi: 10.1016/0145-305x(87)90013-9.
  30. Wen Y., Peng D., Li C., Hu X., Bi S., Song L., Peng B., Zhu J., Chen Y., Yu R.: A new polysaccharide isolated from Morchella importuna fruiting bodies and its immunoregulatory mechanism. Int J Biol Macromol 2019, 137, 8–19, doi: 10.1016/j.ijbiomac.2019.06.171.
  31. Wu Y.; Lan C.; Ren D., Chen G.-Y.: Induction of siglec-1 by endotoxin tolerance suppresses the innate immune response by promoting TGF-β1 production. J Biol Chem 2016, 291, 12370–12382, doi: 10.1074/jbc.M116.721258.
  32. Vogel H.J., Brockbank R.L.: Structure of the oligosaccharide of hen phosvitin as determined by two-dimensional 1H NMR of the intact glycoprotein. Biochemistry 1990, 29, 5574–5583, doi: 10.1021/bi00475a023.
  33. Xie C., Lin X., Hu J., Wang S., Wu J., Xiong W., Wu L.: The polysaccharide from Camellia oleifera fruit shell enhances immune responses via activating MAPKs and NF-κB signaling pathways in RAW264.7 macrophages. Food Nutr Res 2022, 66, 8963, doi: 10.29219/fnr.v66.8963.
  34. Zagulski T., Lipiński P., Zagulska A., Jarząbek Z.: Antibacterial system generated by lactoferrin in mice in vivo is primarily a killing system. Int J Exp Pathol 1998, 79, 117–123, doi: 10.1046/j.1365-2613.1998.00058.x.
  35. Zambrowicz A., Kapczyńska K., Kania P., Nowak J.S., Kaszowska M., Szymczak-Kulus K., Kazana-Płuszka W., Piksa M., Górska S., Jakubczyk D., Macała J., Zabłocka A.: Unravelling the potential of yolkin for nutraceutical use: the origin, structure, and functional insights of a hen egg yolk polypeptide complex. Food Funct 2024, 15, 10746–10760, doi: 10.1039/d4fo03023k.
  36. Zhao M., Qin S., Wang J., Zheng S., Ma X., Xu W.: Cirsii Herba glycoprotein promotes macrophage M1 polarization through MAPK and NF-κB signaling pathways via interaction with TLR4. Int J Biol Macromol 2025, 296, 139687, doi: 10.1016/j.ijbiomac.2025.139687.
  37. Zimecki M., Artym J., Kochanowska I., Kocięba M., Zaczyńska E., Zambrowicz A., Bobak Ł.: Immunoregulatory effects of yolkin on cell proliferation, cytokine production and cell signaling. Braz J Poult Sci 2025, 27, 001–014, doi: 10.1590/1806-9061-2024-2027.
  38. Zimecki M., Artym J., Kocięba M., Weber-Dąbrowska B., Lusiak-Szelachowska M., Górski A.: The concerted action of lactoferrin and bacteriophages in the clearance of bacteria in sublethally infected mice. Post Hig Med Dośw 2008, 62, 42–46.
  39. Zimecki M., Kruzel M.L., Hwang S.-A., Wilk K.M., Actor J.K.: Lactoferrin as an adjuvant for the generation of delayed type hypersensitivity to orally administered antigen. Ann Clin Lab Sci 2021, 51, 359–367.
DOI: https://doi.org/10.2478/jvetres-2026-0056 | Journal eISSN: 2450-8608 (formerly 2300-3235)
Language: English
Submitted on: Mar 3, 2026
Accepted on: Oct 26, 2026
Published on: Nov 2, 2026
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services

© 2026 Michał Zimecki, Jolanta Artym, Maja Kocięba, Ewa Zaczyńska, Aleksandra Zambrowicz, Łukasz Bobak, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.