Fig. 1.
The effects of yolkin on viability of RAW 264.7 cells. The results of one of the two separate experiments conducted are presented as the mean OD at 550/630 nm ±SD from five determinations for each concentration. Ctrl – control incubation of RAW 264.7 cells without yolkin
Fig. 2.
The effects of yolkin on the capability of RAW 264.7 cells to internalise neutral red (NR). (A) Addition of yolkin and NR simultaneously to the culture (no preincubation step). The means ±SD from three replicates of the experiment are presented. (B) Addition of NR after preincubation with yolkin for 48 h. The means ±SD from seven replicates of the experiment are presented. * – Statistical significance (P-value < 0.05) compared to the control (RAW 264.7 cells in culture medium only); Ctrl – control incubation; pre48h – incubation with yolkin for 48 h
Fig. 3.
The effects of yolkin on the capability of RAW 264.7 cells to engulf neutral red (NR)-stained zymosan particles. (A) Yolkin and NR-zymosan were added simultaneously to the culture (no preincubation step). The means ±SD from three replicates of the experiment are presented. (B) Addition of NR-zymosan after preincubation with yolkin for 48 h. The means ±SD from four replicates of the experiment are presented. * – statistical significance (P-value < 0.05) compared to the control (RAW 264.7 cells in culture medium only); Ctrl – control incubation; pre48h – incubation with yolkin for 48 h
Fig. 4.
The effect of incubation of RAW 264.7 cells for 48 h with yolkin on cytokine production. (A) Production of TNF α. (B) Production of IL-6. (C) Production of IL-10. A culture of RAW 264.7 cells was incubated overnight, yolkin was added and the cells incubated for 48 h. The results are presented as the mean ±SD from three independent experiments. Statistical significance is indicated by asterisks (* – P-value < 0.05) compared to the control (RAW 264.7 cells in culture medium only). Ctrl – control incubation