Fig. 1
Canned fish by batch exhibiting bulging and organoleptic changes
Fig. 2
Canned fish samples by batch in which the presence of anaerobes was revealed
Fig. 3
Isolated Gram-positive Clostridium bacteria with subterminal spores
Table 1
Results of the 16S rDNA gene identification of isolates from canned fish
| Amplicon sample | Sequence ID | % Similarity | Similar sequence from the GenBank database |
|---|---|---|---|
| S1 | MT356160.1 | 98.40 | Clostridium sporogenes strain SM5 16S rRNA |
| S2 | MT356160.1 | 99.25 | Clostridium sporogenes strain SM5 16S rRNA |
| S3 | MT356160.1 | 99.15 | Clostridium sporogenes strain SM5 16S rRNA |
| S4 | MT356160.1 | 99.43 | Clostridium sporogenes strain SM5 16S rRNA |
| S5 | MT356160.1 | 99.32 | Clostridium sporogenes strain SM5 16S rRNA |
| S6 | MT356160.1 | 99.51 | Clostridium sporogenes strain SM5 16S rRNA |