Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

Markiewicz-Żukowska, Renata; Naliwajko, Sylwia K.; Bartosiuk, Emilia; Moskwa, Justyna; Isidorov, Valery; Soroczyńska, Jolanta; Borawska, Maria H.

doi:10.2478/jas-2013-0025

Abstract

Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 μg/mL) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 - 66%.

References

Abouda Z., Zerdani I., Kalalou I., Faid M., Ahami M. T. (2011) The antibacterial activity of Moroccan bee bread and bee-pollen (fresh and dried) against pathogenic bacteria. Research Journal of Microbiology 6(4): 376-384. DOI: 10.3923/jm.2011.376.38410.3923/jm.2011.376.384
Search in Google Scholar
Agnihotri S., Burrell K. E., Wolf A., Jalali S., Hawkins C., Rutka J. T., Zadeh G. (2013) Glioblastoma, a brief review of history, molecular genetics, animal models and novel therapeutic strategies. Archivum Immunologiae et Therapiae Experimentalis (Warsz.) 61(1): 25-41. DOI: 10.1007/s00005-012-0203-010.1007/s00005-012-0203-023224339
Search in Google Scholar
Alencar S. M., Oldoni T. L., Castro M. L., Cabral I. S., Costa-Neto C. M., Cury J. A., Rosalen P. L., Ikegaki M. (2007) Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. Journal of Ethnopharmacology 113(2): 278-283. DOI: 10.1016/j.jep.2007.06.00510.1016/j.jep.2007.06.00517656055
Search in Google Scholar
Aljadi A. M., Kamaruddin M. Y. (2004) Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chemistry 85(4): 513-518. DOI: 10.1016/S0308-8146(02)005
Search in Google Scholar
Audisio M. C., Terzolo H. R., Apella M. C. (2005) Bacteriocin from honeybee beebread Enterococcus avium, active against Listeria monocytogenes. Applied and Environmental Microbiology 71(6): 3373-3375. DOI: 10.1128/AEM.71.6.3373-3375
Search in Google Scholar
Baltrušaitytė V., Venskutonis P. R., Čeksterytė V. (2007a) Antibacterial activity of honey and beebread of different origin against S. aureus and S. epidermidis. Food Technology and Biotechnology 45(2): 201-208.
Search in Google Scholar
Baltrušaitytė V., Venskutonis P. R., Čeksterytė V. (2007b) - Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chemistry 101(2): 502-514. DOI: 10.1016/j. foodchem.2006.02.007
Search in Google Scholar
Barbarić M., Mišković K., Bojić M., Lončar M. B., Smolčić-Bubalo A., Debeljak Z., Medić-Šarić M. (2011) Chemical composition of the ethanolic propolis extracts and its effect on HeLa cells. Journal of Ethnopharmacology 135(3): 772-778. DOI: 10.1016/j.jep.2011.04.01510.1016/j.jep.2011.04.01521515353
Search in Google Scholar
Benatti P., Peluso G., Nicolai R., Calvani M. (2004) Polyunsaturated fatty acids: biochemical, nutritional and epigenetic properties. Journal of the American College of Nutrition 23(4): 281-302. DOI: 10.1080/07315724.2004.1071937110.1080/07315724.2004.1071937115310732
Search in Google Scholar
Borawska M. H., Czechowska S. K., Markiewicz R., Socha K., Nazaruk J., Palka J., Isidorov V. A. (2010) Enhancement of antibacterial effects of extracts from Cirsium species using sodium picolinate and estimation of their toxicity. Natural Product Research 24(6): 554-561. DOI: 10.1080/1478641090277072810.1080/1478641090277072820397105
Search in Google Scholar
Borges K. S., Brassesco M. S., Scrideli C. A., Soares A. E., Tone L. G. (2011) Antiproliferative effects of Tubibee propolis in glioblastoma cell lines. Genetics and Molecular Biology 34(2): 310-314. DOI: 10.1590/ S1415-47572011000200024
Search in Google Scholar
Cabral I. S. R., Oldoni T. L. C., Prado A., Bezerra R. M. N., Alencar S. M., Ikegaki M., Rosalen P. L. (2009) Phenolic composition, antibacterial and antioxidant activities of Brazilian red propolis. Quimica Nova 32(6): 1523-1527. DOI: 10.1590/S0100-4042200900060003110.1590/S0100-40422009000600031
Search in Google Scholar
Carmichael J., DeGraff W. G., Gazdar A. F., Minna J. D., Mitchell J. B. (1987) Evaluation of a tetrazoliumbased semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Research 47(4): 936-942.
Search in Google Scholar
Čeksterytė V., Račys J., Kaškonienė V. Venskutonis P. R. (2008) Fatty acid composition in beebread. Biologija 54(4): 253-257. DOI: 10.2478/v10054-008-0052-210.2478/v10054-008-0052-2
Search in Google Scholar
Cimini A., Cristiano L., Colafarina S., Benedetti E., Di Loreto S., Festuccia C., Amicarelli F., Canuto R. A., Cerù M. P. (2005) PPARgamma-dependent effects of conjugated linoleic acid on the human glioblastoma cell line (ADF). International Journal of Cancer 117(6): 923-33. DOI: 10.1002/ijc.2127210.1002/ijc.2127215986437
Search in Google Scholar
Da Silva Frozza C. O., Garcia C. S., Gambato G., de Souza M. D., Salvador M., Moura S., Padilha F.F., Seixas F. K., Collares T., Borsuk S., Dellagostin O. A., Henriques J. A., Roesch-Ely M. (2012) Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis. Food and Chemical Toxicology 52: 137-142. DOI: 10.1016/j.fct.2012.11.01310.1016/j.fct.2012.11.01323174518
Search in Google Scholar
Djeridane A., Yousfi M., Nadjemi B., Boutassouna D., Stoker P., Vidal N. (2006) Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry 97(4): 654-660. DOI: 10.1016/j.foodchem.2005.04.02810.1016/j.foodchem.2005.04.028
Search in Google Scholar
Gangemi R. M., Griffero F., Marubbi D., Perera M., Capra M. C., Malatesta P., Ravetti G. L., Zona G. L., Daga A., Corte G. (2009) SOX2 silencing in glioblastoma tumor- initiating cells causes stop of proliferation and loss of tumorigenicity. Stem Cells 27(1): 40-48. DOI: 10.1634/stemcells.2008-049310.1634/stemcells.2008-049318948646
Search in Google Scholar
Gheldof N., Wang X. H., Engeseth N. J. (2002) Identification and quantification of antioxidant components of honeys from various floral sources. Journal of Agricultural and Food Chemistry 50(21): 5870-5877. DOI: 10.1021/jf025613510.1021/jf025613512358452
Search in Google Scholar
Gilliam M. (1979) Microbiology of pollen and bee bread: the yeasts. Apidologie 10(1): 43-53. DOI: 10.1051/apido:1979010610.1051/apido:19790106
Search in Google Scholar
Gómez-Caravaca A. M., Gómez-Romero M., Arráez- Román D., Segura-Carretero A., Fernández-Gutiérrez. A. (2006) Advances in the analysis of phenolic compounds in products derived from bees. Journal of Pharmaceutical and Biomedical Analysis 41(4): 1220-1234. DOI: 10.1016/j.jpba.2006.03.00210.1016/j.jpba.2006.03.00216621403
Search in Google Scholar
Guarini L., Su Z. Z., Zucker S., Lin J., Grunberger D., Fisher P. B. (1992) Growth inhibition and modulation of antigenic phenotype in human melanoma and glioblastomamultiforme cells by caffeic acid phenethyl ester (CAPE). Cell and Molecular Biology 38(5): 513-527.
Search in Google Scholar
Huang W. J., Huang C. H., Wu C. L., Lin J. K., Chen Y. W., Lin C. L., Chuang S. E., Huang C. Y., Chen C. N. (2007) Propolin G, a prenylflavanone, isolated from Taiwanese propolis, induces caspase-dependent apoptosis in brain cancer cells. Journal of Agricultural and Food Chemistry 55(18): 7366-7376. DOI: 10.1021/ jf071057910.1021/jf071057917685631
Search in Google Scholar
Huang W. J., Lin C. W., Lee C. Y., Chi L. L., Chao Y. C., Wang H. N., Chiou B. L., Chen T. J., Huang C. Y., Chen C. N. (2011) NBM-HD-3, a novel histone deacetylase inhibitor with anticancer activity through modulation of PTEN and AKT in brain cancer cells. Journal of Ethnopharmacology 136(1): 156-167. DOI: 10.1016/j.jep.2011.04.03410.1016/j.jep.2011.04.03421530633
Search in Google Scholar
Isidorov V. A., Isidorova A. G., Szczepaniak L., Czyżewska U. (2009) Gas chromatographic-mass spectrometric investigation of the chemical composition of beebread. Food Chemistry 115(3): 1056-1063. DOI:10.1016/j.foodchem.2008.12.02510.1016/j.foodchem.2008.12.025
Search in Google Scholar
Kas’ianenko V. I., Komisarenko I. A., Dubtsova E. A. (2011) Correction of atherogenic dyslipidemia with honey, pollen and bee bread in patients with different body mass. Terapevticheskii Arkhiv 83(8): 58-62.
Search in Google Scholar
Kaškoniene V., Venskutonis P. R., Ceksteryte V. (2008) Composition of volatile compounds of honey of various floral origin and beebread collected in Lithuania. Food Chemistry 111(4): 988-997. DOI: 10.1016/j.foodchem.2008.05.02110.1016/j.foodchem.2008.05.021
Search in Google Scholar
Leaver H. A., Bell H. S., Rizzo M. T., Ironside J. W., Gregor A., Wharton S. B., Whittle I. R. (2002) Antitumour and pro-apoptotic actions of highly unsaturated fatty acids in glioma. Prostaglandins, Leukotrienes and Essential Fatty Acids 66(1): 19-29. DOI: 10.1054/ plef.2001.033610.1054/plef.2001.033612051954
Search in Google Scholar
Maggiora M., Bologna M., Cerù M. P., Possati L., Angelucci A., Cimini A., Miglietta A., Bozzo F., Margiotta C., Muzio G., Canuto R. A. (2004) An overview of the effect of linoleic and conjugated-linoleic acids on the growth of several human tumor cell lines. International Journal of Cancer 112(6): 909-919. DOI: 10.1002/ijc.2051910.1002/ijc.2051915316938
Search in Google Scholar
Markiewicz-Żukowska R., Car H., Naliwajko S. K., Sawicka D., Szynaka B., Chyczewski L., Isidorov V., Borawska M. H. (2012) Ethanolic extract of propolis, chrysin, CAPE inhibit human astroglia cells. Advancesin Medical Sciences 57(2): 208-216. DOI: 10.2478/ v10039-012-0042-610.2478/v10039-012-0042-623183767
Search in Google Scholar
Morais M., Moreira L., Feas X., Estevinho L. M. (2011) Honeybee-collected pollen from five Portuguese Natural Parks: palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food and Chemical Toxicology 49(5): 1096-1101. DOI: 10.1016/j.fct.2011.01.02010.1016/j.fct.2011.01.02021291944
Search in Google Scholar
Mutsaers M., van Blitterswijk H., van ‘t Leven L., Kerkvliet J., van de Waerdt J. (2005) Bee bread. In: Mutsaers M. (Ed.), Bee products properties, processing and marketing. Agromisa Foundation. Wageningen. pp. 34-35.
Search in Google Scholar
Sulaiman G. M., Al Sammarrae K. W., Ad’hiah A. H., Zucchetti M., Frapolli R., Bello E., Erba E., D’Incalci M., Bagnati R. (2011) Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials. Food and Chemical Toxicology 49(9): 2415-2421. DOI: 10.1016/j.fct.2011.06.06010.1016/j.fct.2011.06.06021723909
Search in Google Scholar
Watanabe M. A., Amarante M. K., Conti B. J., Sforcin J. M. (2011) Cytotoxic constituents of propolis inducing anticancer effects: a review. Journal of Pharmacy and Pharmacology 63(11): 1378-1386. DOI: 10.1111/j.2042-7158.2011.01331.x 10.1111/j.2042-7158.2011.01331.x21988419
Search in Google Scholar

Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

Abstract

Paradigm

My account