Heat stress-induced metabolic responses enhance antioxidant capacity, phenolic and flavonoid accumulation in Bacopa monnieri callus cultures
References
- Angon, P. B., Das, A., Roy, A. R., Khan, J. J., Ahmad, I., Biswas, A., Pallob, T. A., Mondol, M., and Yeasmin, T. S. (2024). Plant development and heat stress: Role of exogenous nutrients and phytohormones in thermotolerance. Discover Plants, 1, 17, https://doi.org/10.1007/s44372-024-00020-3.
- Banerjee, S., Anand, U., Ghosh, S., Ray, D., Ray, P., Nandy, S., Deshmukh, G. D., Tripathi, V., and Dey, A. (2021). Bacosides from Bacopa monnieri extract: An overview of the effects on neurological disorders. Phytotherapy Research, 35(10), 5668–5679, https://doi.org/10.1002/ptr.7203.
- Bapat, V. A., Kavi Kishor, P. B., Jalaja, N., Jain, S. M., and Penna, S. (2023). Plant cell cultures: Biofactories for the production of bioactive compounds. Agronomy, 13(3), 858, https://doi.org/10.3390/agronomy13030858.
- Chandran, H., Meena, M., Barupal, T., and Sharma, K. (2020). Plant tissue culture as a perpetual source for production of industrially important bioactive compounds. Biotechnology Reports, 26, e00450, https://doi.org/10.1016/j.btre.2020.e00450.
- Dionisio-Sese, M. L., and Tobita, S. (1998). Antioxidant responses of rice seedlings to salinity stress. Plant Science, 135, 1–9, https://doi.org/10.1016/S0168-9452(98)00025-9.
- Espinosa-Leal, C. A., Puente-Garza, C. A., and García-Lara, S. (2018). In vitro plant tissue culture: Means for production of biologically active compounds. Planta, 248(1), 1–18, https://doi.org/10.1007/s00425-018-2910-1.
- Fatima, U., Roy, S., Ahmad, S., Al-Keridis, A. L., Alshammari, N., Adnan, M., Islam, A., and Hassan, I. M. (2022). Investigating neuroprotective roles of Bacopa monnieri extracts: Mechanistic insights and therapeutic implications. Biomedicine and Pharmacotherapy 153, 113469, https://doi.org/10.1016/j.biopha.2022.113469.
- Hasanuzzaman, M., Bhuyan, M. H. M. B., Zulfiqar, F., Raza, A., Mohsin, S. M., Mahmud, J. A., Fujita, M., and Fotopoulos, V. (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants, 9(8), 681, https://doi.org/10.3390/antiox9080681.
- Heath, R. L., and Packer, L. (1968). Photoperoxidation in isolated chloroplasts: II. Role of electron transfer. Archives of Biochemistry and Biophysics, 125, 850–857, https://doi.org/10.1016/0003-9861(68)90523-7.
- Isah, T. (2019). Stress and defense responses in plant secondary metabolites. Biological Research, 52, 39, https://doi.org/10.1186/s40659-019-0246-3.
- Kan, Y., Mu, X.-R., Gao, J., Lin, H. X., and Lin, Y. (2023). The molecular basis of heat stress responses in plants. Molecular Plant, 16(10), 1612–1634, https://doi.org/10.1016/j.molp.2023.09.013.
- Koul, A., and Mallubhotla, S. (2020). Elicitation and enhancement of bacoside production using suspension cultures of Bacopa monnieri (L.) Wettst. 3 Biotech, 10(6), 256, https://doi.org/10.1007/s13205-020-02242-0.
- Kumari, A., Kumar, A., Mandal, S., Roy, P., and Sircar, D. (2023). A metabolic reprogramming in Bacopa monnieri induced by methyl-jasmonate and enhanced biosynthesis of triterpene saponins. Industrial Crops and Products, 204(5), 117241, https://doi.org/10.1016/j.indcrop.2023.117241.
- Mathur, D., Goyal, K., Koul, V., and Anand, A. (2016). The molecular links of re-emerging therapy: A review of evidence of Brahmi (Bacopa monnieri). Frontiers in Pharmacology, 7, 44, https://doi.org/10.3389/fphar.2016.00044.
- Medina, E., Kim, S. H., Yun, M., and Choi, W. G. (2021). Recapitulation of the function and role of ROS generated in response to heat stress in plants. Plants, 10(2), 371, https://doi.org/10.3390/plants10020371.
- Mittler, R., Zandalinas, S. I., Fichman, Y., and Van Breusegem, F. (2022). Reactive oxygen species signalling in plant stress responses. Nature Reviews Molecular Cell Biology, 23, 663–679, https://doi.org/10.1038/s41580-022-00499-2.
- Mondal, S., Karmakar, S., Panda, D., Pramanik, K., Bose, B., and Singhal, K. R. (2023). Crucial plant processes under heat stress and tolerance through heat shock proteins. Plant Stress, 10, 100227, https://doi.org/10.1016/j.stress.2023.100227.
- Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497, https://doi.org/10.1111/j. 1399-3054.1962.tb08052.x
- Mwamatope, B., Tembo, D., Chikowe, I., Kampira, E., and Nyirenda, C. (2020). Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants. Scientific African, 9, e00481, https://doi.org/10.1016/j.sciaf.2020.e00481.
- Naik, P. M., and Al-Khayri, J. M. (2016). Abiotic and biotic elicitors-Role in secondary metabolites production through in vitro culture of medicinal plants. In A. K. Shanker and C. Shanker (Eds.), Abiotic and Biotic Stress in Plants – Recent Advances and Future Perspectives (pp. 247–277). InTech, https://doi.org/10.5772/61442.
- Noctor, G., Reichheld, J.-P., and Foyer, C. H. (2018). ROS-related redox regulation and signaling in plants. Seminars in Cell and Developmental Biology, 80, 3–12, https://doi.org/10.1016/j.semcdb.2017.07.013.
- Ohama, N., Sato, H., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2017). Transcriptional regulatory network of plant heat stress response. Trends in Plant Science, 22, 53–65, https://doi.org/10.1016/j.tplants.2016.08.015.
- Pekal, A., and Pyrzynska, K. (2014). Evaluation of aluminium complexation reaction for flavonoid content assay. Food Analytical Methods, 7, 1776–1782, https://doi.org/10.1007/s12161-014-9814-x.
- Petrov, V., and Gechev, T. (2023). ROS and abiotic stress in plants 2.0. International Journal of Molecular Sciences, 24(15), 11917, https://doi.org/10.3390/ijms241511917.
- Razzaq, A., Zafar, M. M., Ali, A., Ihsan, L., Qadir, F., Khan, M. N., Zhang, Y., Gao, L., Cong, H., Iqbal, R., Jiang, X., and Qiao, F. (2025). Elicitor-mediated enhancement of secondary metabolites in plant species: A review. Frontiers in Plant Science, 16, 1706600, https://doi.org/10.3389/fpls.2025.1706600.
- Sekhar, V. C., Viswanathan, G., and Baby, S. (2019). Insights into the molecular aspects of neuroprotective bacoside A and bacopaside I. Current Neuropharmacology, 17(5), 438–446, https://doi.org/10.2174/1570159X16666180419123022.
- Sharma, A., Kumar, V., Shahzad, B., Ramakrishnan, M., Sidhu, G. P. S., Bali, S. A., Handa, N., Kapoor, D., Yadav, P., Khanna, K., Bakshi, P., Rehman, A., Kohli, S. K., Khan, A. E., Parihar, D. R., Yuan, H., Thukral, A. K., Bhardwaj, R., and Zheng, B. (2020). Photosynthetic response of plants under different abiotic stresses: A review. Journal of Plant Growth Regulation, 39, 509–531, https://doi.org/10.1007/s00344-019-10018-x.
- Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., and Zheng, B. (2019). Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress. Molecules, 24(13), 2452, https://doi.org/10.3390/molecules24132452.
- Sharma, M., Ahuja, A., Gupta, R., and Mallubhotla, S. (2015). Enhanced bacoside production in shoot cultures of Bacopa monnieri under the influence of abiotic elicitors. Natural Product Research, 29(8), 745–749, https://doi.org/10.1080/14786419.2014.986657.
- Sharma, P., Yadav, S., Srivastava, A., and Shrivastava, N. (2013). Methyl jasmonate mediates upregulation of bacoside A production in shoot cultures of Bacopa monnieri. Biotechnology Letters, 35(7), 1121–1125, https://doi.org/10.1007/s10529-013-1178-6.
- Simpson, T., Pase, M., and Stough, C. (2015). Bacopa monnieri as an antioxidant therapy to reduce oxidative stress in the aging brain. Evidence-Based Complementary and Alternative Medicine : eCAM, 2015, 615384, https://doi.org/10.1155/2015/615384.
- Taratima, W., Chuanchumkan, C., Maneerattanarungroj, P., Trunjaruen, A., Theerakulpisut, P., and Dongsansuk, A. (2022). Effect of heat stress on some physiological and anatomical characteristics of rice (Oryza sativa L.) cv. KDML105 callus and seedling. Biology (Basel), 11, 1587, https://doi.org/10.3390/biology11111587.
- Taratima, W., Trunjaruen, A., Kaenpho, K., Maneerattanarungroj, P., and Kunpratum, N. (2025). Physiological and metabolic responses of Bacopa monnieri to salt-induced stress: Implications for secondary metabolite elicitation. BMC Plant Biology, 25(1), 991, https://doi.org/10.1186/s12870-025-07037-0.
- Valotto Neto, L. J., Reverete De Araujo, M., Moretti Junior, R. C., Mendes Machado, N., Joshi, R. K., Dos Santos Buglio, D., Barbalho Lamas, C., Direito, R., Fornari Laurindo, L., Tanaka, M., and Barbalho, S. M. (2024). Investigating the neuroprotective and cognitive-enhancing effects of Bacopa monnieri: A systematic review. Antioxidants, 13(4), 393, https://doi.org/10.3390/antiox13040393.
- Xu, Y., and Fu, X. (2022). Reprogramming of plant central metabolism in response to abiotic stresses: A metabolomics view. International Journal of Molecular Sciences, 23(10), 5716, https://doi.org/10.3390/ijms23105716.
- Zhao, J., Lu, Z., Wang, L., and Jin, B. (2021). Plant responses to heat stress: Physiology, transcription, noncoding RNAs, and epigenetics. International Journal of Molecular Sciences, 22(1), 117, https://doi.org/10.3390/ijms22010117.
Language: English
Submitted on: May 5, 2026
Accepted on: Jul 6, 2026
Published on: Jul 29, 2026
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
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© 2026 Prathan Luecha, Narisa Kunpratum, Attachai Trunjaruen, Wipa Yaowachai, Pitakpong Maneerattanarungroj, Worasitikulya Taratima, published by Polish Society for Horticultural Sciences (PSHS)
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