
Figure 1
Experimental plant and UV-B light exposure: a. Trigonella plants; b. Plants under white light; c. Plants under UV-B light.

Figure 2
Effect of UV -B exposure on photosynthetic pigment contents of Trigonella: a. Chlorophyll a; b. Chlorophyll b; c. Total chlorophyll; d. Carotenoids.

Figure 3
UV- B exposure related changes of bioactive compounds in herbal extract of Trigonella: a. Anthocyanin; b. Flavanoids; c. Phenolics; d. Total antioxidant activity.

Figure 4
Effect of UV Antioxidant enzyme activies (a. Phenylalanine lyase; b. Guiacol peroxidase) and total lipid content of test plants up on UV- B exposure.

Figure 5
GC-MS spectra revealing effect of UV- B radiation on essential oil composition of Trigonella. a. Control plants; b. UV- B exposed plants (4.0 hr).
Table 1
UV B induced changes in chemical composition of Trigonella leaves
| Peak No. | R.T.(s) | Area% | Control | Formula |
|---|---|---|---|---|
| 98 | 1830.4 | -11.534 | Phytol | C20H40O |
| 137 | 2226 | -15.255 | Nonadecane,2-methyl | C20H42 |
| 165 | 2549.8 | -8.697 | Nonacos-1-ene | C29H58 |
| 99 | 1525.4 | +1.8477 | 2-Dodecanol,2-methyl | C13H28O |
| 100 | 1534 | +9.7588 | 2- Pentadecanone,6,10,14-trimethyl | C18H36O |
| 115 | 1626.2 | +1.5965 | Pentadecanoic acid,14-methyl-,methylester | C17H34O2 |
| 143 | 1909.4 | +2.2113 | 11-methyldodecanol | C13H28O |
| 170 | 2230.2 | +1.8169 | Octadecane,2-methyl | C19H40 |
| 185 | 2564.4 | +4.6159 | n-Tetracosanol-1 | C24H50O |
| 193 | 2720.8 | +1.7377 | Eicosane,2-methyl | C21H44 |
[i] - indicate decrease and + indicate increase