Screening and Identification of New Types of Exopolysaccharides-Producing Lactic Acid in the Inner Mongolia Dairy Products

Shangguan, Wenfei; Chen, He; Li, Yilin; Wang, Zihua; Guo, Hongxing; Meng, Jiangpeng

doi:10.2478/aucft-2019-0010

Abstract

Exopolysaccharides (EPS) is a type of polysaccharide produced by lactic acid bacteria (LAB) that can be directly used in foods to make the products more excellent. Therefore, batch studies were performed to explore the effect of different LAB on the production of EPS and antioxidant activity. Five strains with high EPS yield and antioxidant activity were screened out from 66 strains isolated from Tibetan dairy products. The results show that EPS produntion of the five strains (B55, B62, B30, 7830 and K2) were 110.66, 145.48, 132.78, 122.11 and 111.72 mg·L⁻¹, respectively, and they have a higher DPPH free radical scavenging activity (56.29, 66.43, 62.94, 68.71, 61.87%). Five LAB strains were identified and classified based on screening, purification and 16S rDNA sequences. Molecular characterization based on partial sequence 16S rDNA homology confirmed the initial identification as Lactobacillus fermentum (B55, B62), Lactobacillus plantarum (7830), Pediococcus acidilactici (B30) and Lactobacillus helveticus (K2).

References

1. Coton, M.; Berthier, F.; Coton, E. (2008). Rapid identification of the three major species of dairy obligate heterofermenters Lactobacillus brevis, Lactobacillus fermentum & Lactobacillus parabuchneri by species-specific duplex PCR. Fems Microbiol Lett. 2008, 284, 150-157. DOI: 10.1111/j.1574-6968.2008.01206.x.10.1111/j.1574-6968.2008.01206.x18510560
Open DOI Search in Google Scholar Back to article
2. Coudeyras, S.; March&in, H.; Fajon, C.; Forestier, C. (2008). Taxonomic & strain-specific identification of the probiotic strain Lactobacillus rhamnosus 35 within the Lactobacillus casei group. Appl Environ Microbiol. 2008, 74, 2679-2689. DOI: 10.1128/aem.02286-07.10.1128/aem.02286-07239486818326671
Open DOI Search in Google Scholar Back to article
3. Dubois, M.; Gilles, K.; Hamilton, J.; Rebers, P.; Smith, F. (1956). Colorimetric methods for determination of sugars and related substances. Analytical Chemistry. 1956, 28, 350–356. DOI: 10.1021/ac60111a017.10.1021/ac60111a017
Open DOI Search in Google Scholar Back to article
4. Fukuda, K.; Shi, T.; Nagami, K. (2010). Effects of carbohydrate source on physicochemical properties of the exopolysaccharide produced by Lactobacillus fermentum, TDS030603 in a chemically defined medium. Carbohydrate Polymers. 2010, 79(4), 1040-1045. DOI: 10.1016/j.carbpol.2009.10.037.10.1016/j.carbpol.2009.10.037
Open DOI Search in Google Scholar Back to article
5. Girard, M.; Sehaffer-Lequart, C.(2007). Attractive in ieractions between selected anionic exopolysaecharides and milk proteins. Food Hydroeolloids. 2007, 9, 1–10. DOI: 10.1016/j.foodhyd.2007.09.001.10.1016/j.foodhyd.2007.09.001
Open DOI Search in Google Scholar Back to article
6. Goranov, B.; Denkova, R.; Teneva, D. (2015). Molecular-genetic identification of Lactobacillus strains, isolated from homemade yoghurt. Ukrainian Food J. 2015, 4(1), 67–76.
Search in Google Scholar Back to article
7. Li, W.; Ji, J.; Rui, X. (2014). Production of exopolysaccharides by Lactobacillus helveticus, MB2-1 and its functional characteristics invitro. LWT - Food Science and Technology. 2014, 59(2), 732-739. DOI: 10.1016/j.lwt.2014.06.063.10.1016/j.lwt.2014.06.063
Open DOI Search in Google Scholar Back to article
8. Mende, S.; Peter, M.; Bartels, K.; et al.(2013) Addition of purified exopolysaccharide isolates from S. thermophilus to milk and their impact on the rheology of acid gels. Food Hydroeolloids, 2013, 32, 178–185. DOI: 10.1016/j.foodhyd.2012.12.011.10.1016/j.foodhyd.2012.12.011
Search in Google Scholar Back to article
9. Meng, X.S.; Li, B. (2012). Antioxidant activity of Lactobacillus paracase and its exopolysaccharides. Food Sci Tech, 2012, 37(09), 48–53. DOI: 10.13684/j.cnki.spkj.2012.09.001.
Search in Google Scholar Back to article
10. Ouwehand, A.; Salminen, S.; Isolauri, E.(2002). Probiotics: an overview of beneficial effects. Antonie van Leeuwenhoek. 2002, 82, 279-289. DOI: 10.1023/a:1020620607611.10.1023/A:1020620607611
Search in Google Scholar Back to article
11. Parvez, S.; Malik, K.A.; Kang, S.A.; Kim, H.Y.(2006). Probiotics and their fermented food products are beneficial for health. J Appl Microbiol. 2006, 100, 1171-1185. DOI: 10.1111/j.1365-2672.2006.02963.x.10.1111/j.1365-2672.2006.02963.x16696665
Open DOI Search in Google Scholar Back to article
12. PRASANNA, P.H.P.; GRANDISON, A.S.; CHARALAMPOPOULOS, D. (2013). Screening human intestinal Bifidobacterium strains for growth, acidification, EPS production and viscosity potential in low fat milk. Int Dairy J, 2012, 23(1), 36-44. DOI: 10.1016/j.idairyj.2011.09.008.10.1016/j.idairyj.2011.09.008
Open DOI Search in Google Scholar Back to article
13. Pu, Z.Y.; Dobos, M.; Limsowtin, G. K. Y.; Powell, I. B. (2002). Integrated polymerase chain reaction-based procedures for the detection and identification of species and subspecies of the Gram-positive bacterial genus Lactococcus. J Appl Microbiol. 2002, 93, 353-361.DOI: 10.1046/j.1365-2672.2002.01688.x.10.1046/j.1365-2672.2002.01688.x12147085
Open DOI Search in Google Scholar Back to article
14. Purwandari, U.; Shah, N.P.; Vasiljevic, T.(2007). Effects of exopolysaccharide-producing strains of Streptococcus thermophilus on technological and rheological properties of set-type yoghurt. Int Dairy J, 2007, 17, 1344–1352. DOI: 10.1016/j.idairyj.2007.01.018.10.1016/j.idairyj.2007.01.018
Open DOI Search in Google Scholar Back to article
15. Savadogo, A.C.A.T.; Ouattara1, Savadogo, P.W.; Barro, N.; Ouattara, A.S.; Traoré, A.S.(2004). Identification of exopolysaccharides-producing lactic acid bacteria from Burkina Faso fermented milk samples. Afr J Biotechnol. 2004, 3, 189-194. DOI: 10.5897/AJB2004.000-2034.10.5897/AJB2004.000-2034
Open DOI Search in Google Scholar Back to article
16. Schillinger, U.; Boehringer, B.; Wallbaum, S.; Caroline, L.; Gonfa, A.; Huch, M.; Holzapfel, W. H.; Franz, C. (2008). A genus-specific PCR method for differentiation between Leuconostoc and Weissella and its application in identification of heterofermentative lactic acid bacteria from coffee fermentation. Fems Microbio Lett. 2008, 286, 222–226. DOI: 10.1111/j.1574-6968.2008.01286.x.10.1111/j.1574-6968.2008.01286.x18657111
Open DOI Search in Google Scholar Back to article
17. Sokal, R.R. (1958). A statistical method of evaluating systematic relationships. Univ Kansas Sci Bull. 1958, 38, 1409–1438.
Search in Google Scholar Back to article
18. Song, Y.R.; Jeong, D.Y.; Cha, Y.S. (2013). Exopolysaccharide produced by Pediococcus acidilactici M76 isolated from the Korean traditional rice wine, makgeolli. J Microbiol Biotechnol. 2013, 23(5), 681-688. DOI: 10.4014/jmb.1301.01032.10.4014/jmb.1301.0103223648859
Open DOI Search in Google Scholar Back to article
19. Sun, G.; Yao, T.; Feng, C. (2017). Identification and biocontrol potential of antagonistic bacteria strains against Sclerotinia sclerotiorum, and their growth-promoting effects on Brassicanapus. Biol Control. 2017, 104, 35–43. DOI: 10.1016/j.biocontrol.2016.10.008.10.1016/j.biocontrol.2016.10.008
Open DOI Search in Google Scholar Back to article
20. Sutherland, I.W.(2002). A sticky business. Microbial exopolysaccharides: current products and future trends. Microbiology Today. 2002, 29, 70-71.
Search in Google Scholar Back to article
21. Uchida, M.; Ishii, I.; Inoue, C.(2010). Kefiran reduces atherosclerosis in rabbits fed a high cholesterol diet. J Atheroscler Thromb, 2010, 17, 980–988. DOI: 10.5551/jat.4812.10.5551/jat.481220543518
Open DOI Search in Google Scholar Back to article
22. Wang, J.; Zhao, X.; Tian, Z. (2015). Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11 isolated from Tibet Kefir. Carbohyd Polym. 2015, 125, 16-25. DOI: 10.1016/j.carbpol.2015.03.00310.1016/j.carbpol.2015.03.00325857955
Open DOI Search in Google Scholar Back to article
23. Wang, Q.; Sun, Y.; Yang, B.; Wang, Z.; Liu, Y.; Cao, Q.; Sun, X.; Kuang, H. (2014). Optimization of polysaccharides extraction from seeds of Pharbitis nil and itsanti-oxidant activity. Carbohyd. Polym. 2014, 102, 460–466. DOI: 10.1016/j.carbpol.2013.11.068.10.1016/j.carbpol.2013.11.06824507306
Open DOI Search in Google Scholar Back to article
24. Wu, M.H.; Pan, T.M.; Wu, J.Y.(2010). Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties. Int J Food Microbiol. 2010, 144, 104–110. DOI: 10.1016/j.ijfoodmicro.2010.09.003.10.1016/j.ijfoodmicro.2010.09.00320884069
Open DOI Search in Google Scholar Back to article
25. Xu, N.; Xi, A.D. (2012). Study on exopolysaccharide of Lactobacillus casei KW3. J Chine Inst Food Sci Tech, 2012, 12(7), 42–47. DOI: 10.3969/j.issn.1009-7848.2012.07.007.10.3969/j.issn.1009-7848.2012.07.007
Open DOI Search in Google Scholar Back to article
26. Yang, T.; Wu, K.; Wang, F. (2014). Effect of exopolysaccharides from lactic acid bacteria on the texture and microstructure of buffalo yoghurt. Int Dairy J, 2014, 34(2), 252-256. DOI: 10.1016/j.idairyj.2013.08.007.10.1016/j.idairyj.2013.08.007
Open DOI Search in Google Scholar Back to article
27. Yang, Z.N.; Zhang, X.(2007). Rheological characterization and molecular structural modification of exopolysaccharides produced by lactic acid bacteria. Food Sci. 2007, 28(12), 535–538. DOI: CNKI:SUN:SPKX.0.2007-12-132.
Search in Google Scholar Back to article
28. Zhang, L.; Liu, C.Y.; Li, D.(2013). Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88. Int J Biol Macromol, 2013, 54, 270–275. DOI: 10.1016/j.ijbiomac.2012.12.037.10.1016/j.ijbiomac.2012.12.03723274679
Open DOI Search in Google Scholar Back to article
29. Zhang, T.H.; Zhang, C.H.; Li, S.H.(2011). Growth and exopolysaccharide production by Streptococcus thermophilus ST1 in skim milk. Braz J Microbiol, 2011, 42, 1470–1478. DOI: 10.1590/S1517-83822011000400033.10.1590/S1517-83822011000400033
Open DOI Search in Google Scholar Back to article
30. Zhang, Y.C.; Li, S.Y.; Zhang, C.Y.(2011). Growth and exopolysaccharide production by Lactobacillus fermentum F6 in skim milk. Afr J Biotechnol, 2011, 10(11), 2080-2091. DOI: 10.1093/cid/cir036.10.1093/cid/cir03621367740
Open DOI Search in Google Scholar Back to article
31. Zhang, C.L.(2014). Selection of exopolysaccharide-producing lactic acid bacteria isolates from Inner Mongolian traditional yoghurt. Mljekarstvo, 2014 64(4):254-260. DOI: 10.15567/mljekarstvo.2014.0404.10.15567/mljekarstvo.2014.0404
Search in Google Scholar Back to article

Screening and Identification of New Types of Exopolysaccharides-Producing Lactic Acid in the Inner Mongolia Dairy Products

Abstract

Paradigm

My account