Longitudinal Phenotypic and Antimicrobial Resistance Evolution of Mycobacterium Abscessus Subsp. Abscessus During Chronic Pulmonary Infection in a Patient with Cystic fibrosis
References
- Abdelaal HFM, Chan ED, Young L, et al. Mycobacterium abscessus: it’s complex. Microorganisms. 2022 July;10(7):1454.
https://doi.org/10.3390/microorganisms10071454 - Adjemian J, Frankland TB, Daida YG, Honda JR, Olivier KN, Zelazny A, Honda S, Prevots DR. Epidemiology of Nontuberculous Mycobacterial Lung Disease and Tuberculosis, Hawaii, USA. Emerg Infect Dis. 2017 Mar;23(3):439–447.
https://doi.org/10.3201/eid2303.161827 - Bronson RA, Gupta C, Manson AL, Nguyen JA, Bahadirli-Talbott A, Parrish NM, Earl AM, Cohen KA. Global phylogenomic analyses of Mycobacterium abscessus provide context for non-cystic fibrosis infections and the evolution of antibiotic resistance. Nat Commun. 2021 August;12:5145.
https://doi.org/10.1038/s41467-021-25484-9 - Bryant JM, Grogono DM, Rodriguez-Rincon D, Everall I, Brown KP, Moreno P, Verma D, Hill E, Drijkoningen J, Gilligan P, et al. Emergence and spread of a human-transmissible multidrug-resistant nontuberculous mycobacterium. Science. 2016 November;354(6313):751–757.
https://doi.org/10.1126/science.aaf8156 - Curtoni A, Cordovana M, Bondi A, Scaiola F, Criscione G, Ghibaudo D, Pastrone L, Zanotto E, Camaggi A, Caroppo MS, et al. Application of FT-IR spectroscopy for Mycobacterium abscessus complex subspecies differentiation. J Microbiol Methods. 2023 September;212:106792.
https://doi.org/10.1016/j.mimet.2023.106792 - Dartois V, Dick T. Toward better cures for Mycobacterium abscessus lung disease. Clin Microbiol Rev. 2024 December;37(4).
https://doi.org/10.1128/cmr.00080-23 - Egorova A, Jackson M, Gavrilyuk V, Makarov V. Pipeline of anti-Mycobacterium abscessus small molecules: repurposable drugs and promising novel chemical entities. Med Res Rev. 2021 July;41(4):2350–2387.
https://doi.org/10.1002/med.21798 - Ferrell KC, Stewart EL, Counoupas C, Triccas JA. Colony morphotype governs innate and adaptive pulmonary immune responses to Mycobacterium abscessus infection in C3HeB/FeJ mice. Eur J Immunol. 2024 July;54(7).
https://doi.org/10.1002/eji.202350610 - Floto RA, Olivier KN, Saiman L, Daley CL, Herrmann JL, Nick JA, Noone PG, Bilton D, Corris P, Gibson RL, et al. US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis. Thorax. 2016 January;71(Suppl 1)–i22.
https://doi.org/10.1136/thoraxjnl-2015-207360 - Forbes BA, Hall GS, Miller MB, Novak SM, Rowlinson MC, Salfinger M, Somoskövi A, Warshauer DM, Wilson ML. Practical guidance for clinical microbiology laboratories: mycobacteria. Clin Microbiol Rev. 2018 April;31(2).
https://doi.org/10.1128/CMR.00038-17 - GenoType CMdirectVER 1.0 IFU-295-01, Hain Lifescience GmbH, Nehren, Germany:
https://share.google/JaH0oGNmoMDyLtbSZ - GenoType NTM-DR VER 1.0 – Instructions for Use. IFU-297-03. Hain Lifescience GmbH Nehren, Germany:
https://share.google/16YwQIi1UuygpUo3O - CLSI. Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes. 3rd ed. CLSI standard M24. Wayne, PA: Clinical and Laboratory Standards Institute; 2018
- CLSI. Performance Standards for Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes. 2nd edition. CLSI supplement M24S. Clinical and Laboratory Standards Institute; 2023
- Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, Holland SM, Horsburgh R, Huitt G, Iademarco MF, et al. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med. 2007 February;175(4):367–416.
https://doi.org/10.1164/rccm.200604-571ST - Huang Y-L, Huang C-H, Huang Y-C, Yen C-L, Hsu C-R. Anti-biofilm activities and antibiotic synergy of naturally occurring compounds against drug-resistant rapidly growing mycobacteria. Microbiol Spectr. 2024 August;12(8).
https://doi.org/10.1128/spectrum.00199-24 - Jeong SH, Kim SY, Huh HJ, Kim SY, Jeong BH, Lee NY, Shin SJ, Koh W-J. Mycobacteriological characteristics and treatment outcomes in extrapulmonary Mycobacterium abscessus complex infections. Int J Infect Dis. 2017 July;60:49–56.
https://doi.org/10.1016/j.ijid.2017.05.007 - Kania K, Wójcik K, Czekajewska J, Grzesiak M, Klesiewicz K. Molecular identification of strains within the Mycobacterium abscessus complex and determination of resistance to macrolides and aminoglycosides. Pol J Microbiol. 2023 December;72(4):491–506.
https://doi.org/10.33073/pjm-2023-048 - Laencina L, Dubois V, Le Moigne V, Viljoen A, Majlessi L, Proux-Gillardeaux V, Tanguy M, Leroux M, Burguière A, Veyron-Churlet R, et al. Identification of genes required for Mycobacterium abscessus growth in vivo with a prominent role of the ESX-4 locus. Proc Natl Acad Sci U S A. 2018 January;115(5)– E1011.
https://doi.org/10.1073/pnas.1713195115 - Lara-Reyna S, Holbrook J, Jarosz-Griffiths HH, Bianchi V, D’Arcy R, Grierson C, Glover L, Wadsworth M, Corris PA, Roscioli E, et al. Dysregulated signalling pathways in innate immune cells with cystic fibrosis mutations. Cell Mol Life Sci. 2020 November;77(22):4485–4503.
https://doi.org/10.1007/s00018-020-03540-9 - Lee MR, Sheng WH, Hung CC, Yu CJ, Lee LN, Hsueh PR. Mycobacterium abscessus complex infections in humans. Emerg Infect Dis. 2015 September;21(9):1638–1646.
https://doi.org/10.3201/eid2109.141634 - Lira RLS, Nogueira FAB, Campos RFPC, Silva JRA, Rodrigues TMG, Silva KMS, Andrade BB, Oliveira-Neto OB. Mycobacterium abscessus subsp. massiliense: biofilm formation, host immune response, and therapeutic strategies. Microorganisms. 2025 February;13(2):447.
https://doi.org/10.3390/microorganisms13020447 - MBT Mycobacteria Kit – Instructions for Use (Revision A1, Doc. No. 5045500). Bruker Daltonics GmbH & Co. KG.
- Martiniano SL, Nick JA, Daley CL. Nontuberculous mycobacterial infections in cystic fibrosis. Thorac Surg Clin. 2019 February;29(1):95–108.
https://doi.org/10.1016/j.thorsurg.2018.09.008 - Park EJ, Silwal P, Jo EK. Host–pathogen interactions operative during Mycobacteroides abscessus infection. Immune Netw. 2021 December;21(6).
https://doi.org/10.4110/in.2021.21.e40 - Parmar J, Tocheva EI. The mycobacterial cell envelope: a moving target. Trends Microbiol. 2023 March;31(3):255–268.
https://doi.org/10.1016/j.tim.2022.09.006 - Ratnatunga CN, Lutzky VP, Kupz A, Doolan DL, Reid DW, Field M, Bell SC, Thomson RM, Miles JJ. The rise of non-tuberculosis mycobacterial lung disease. Front Immunol. 2020 February;11:303.
https://doi.org/10.3389/fimmu.2020.00303 - Recchia D, Stelitano G, Stamilla A, Trovato C, La Spina S, Scalise G, et al. Mycobacterium abscessus infections in cystic fibrosis individuals: a review on therapeutic options. Int J Mol Sci. 2023 March;24(5):4635.
https://doi.org/10.3390/ijms24054635 - Schmalstig AA, Wiggins A, Badillo D, Johansen MD, Hansen MH, Xiao Y, et al. Bacteriophage infection and killing of intracellular Mycobacterium abscessus. mBio. 2024 January;15(1).
https://doi.org/10.1128/mbio.02924-23 - To K, Cao R, Yegiazaryan A, Owens J, Venketaraman V. General overview of nontuberculous mycobacteria opportunistic pathogens: Mycobacterium avium and Mycobacterium abscessus. J Clin Med. 2020 August;9(8):2541.
https://doi.org/10.3390/jcm9082541 - Victoria L, Gupta A, Gómez JL, Robledo J. Mycobacterium abscessus complex: a review of recent developments in an emerging pathogen. Front Cell Infect Microbiol. 2021 April;11:659997.
https://doi.org/10.3389/fcimb.2021.659997 - World Health Organization. Manual for selection of molecular WHO-recommended rapid diagnostic tests for detection of tuberculosis and drug-resistant tuberculosis. Geneva: World Health Organization; 2022.
- Zomer D, van Ingen J, Hofland R, Dutch CF Registry Steering group, Akkerman OW, Altenburg J, Bakker M, Bannier MAGE, Conemans LH, Gulmans VAM, et al. Epidemiology and management of nontuberculous mycobacterial disease in people with cystic fibrosis, the Netherlands. J Cyst Fibros. 2023 March;22(2):327–333.
https://doi.org/10.1016/j.jcf.2022.10.009
Language: English, Polish
Page range: 161 - 173
Submitted on: Jul 2, 2026
Accepted on: Sep 14, 2026
Published on: Oct 13, 2026
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
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© 2026 Katarzyna Kania, Katarzyna Wójcik, Magdalena Grzesiak, Adrian Szczepański, Iwona Gross-Sondej, Krzysztof Sładek, Karolina Klesiewicz, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.