Primers used for the detection of virulence genes
| Gene | Primer sequence (5′–3′) | Amplicon size (bp) | Reference |
|---|---|---|---|
| SAP1-F | TCAATCAATTTACTCTTCCATTTCTAACA | 161 | Costa 2010 |
| SAP1-R | CCAGTAGCATTAACAGGAGTTTAATGACA | ||
| SAP2-F | AACAACAACCCACTAGACATCACC | 178 | |
| SAP2-R | TGACCATTAGTAACTGGGAATGCTTTAGGA | ||
| SAP4-F | CATTCATTCCTTTAATACCGACTATC | 156 | |
| SAP4-R | GGTAACAAACCCTGTAGATCTTTTAA | ||
| ALS1-F | GACTAGTGAACCAACAAATAC | 318 | Dikmen 2021 |
| ALS1-R | CCAGAAGAAACAGCAGGTGA | ||
| HWP1-F | ATGACTCCAGCTGGTTC | 572 | |
| HWP1-R | TAGATCAAGAATGCAGC | ||
| PLB1-F | ATGATTTTGCATCATTTG | 751 | |
| PLB1-R | AGTATCTGGAGCTCTAC |
Distribution and relationship between genotypes and the presence of virulence genes (n, %)_
| Gene | Total | Genotype A (n=28) | Genotype B (n=14) | Genotype C (n=8) | p value |
|---|---|---|---|---|---|
| SAP1 | 48 (96.0) | 26 (92.8) | 14 (100) | 8 (100) | 0.680 |
| SAP2 | 49 (98.0) | 28 (100) | 13 (92.8) | 8 (100) | 0.439 |
| SAP4 | 49 (98.0) | 28 (100) | 13 (92.8) | 8 (100) | 0.439 |
| ALS1 | 46 (92.0) | 26 (92.8) | 13 (92.8) | 7 (87.5) | 0.801 |
| HWP1 | 46 (92.0) | 25 (89.3) | 13 (92.8) | 8 (100) | 0.999 |
| PLB1 | 47 (94.0) | 26 (92.8) | 13 (92.8) | 8 (100) | 0.999 |