References
- Anderson NW. Peaks of promise and peril: screening for antibiotic resistance by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2016 Jan; 54(1):5–6.
https://doi.org/10.1128/JCM.02490-15 - Astudillo CA, López-Cortés XA, Ocque E, Manríquez-Troncoso JM. Multi-label classification to predict antibiotic resistance from raw clinical MALDI-TOF mass spectrometry data. Sci Rep. 2024 Dec; 14(1): 31283.
https://doi.org/10.1038/s41598-024-82697-w - Burckhardt I, Zimmermann S. Susceptibility testing of bacteria using maldi-tof mass spectrometry. Front Microbiol. 2018 AUg; 9: 1744.
https://doi.org/10.3389/fmicb.2018.01744 - CalderaroA, Chezzi C. MALDI-TOF MS: a reliable tool in the real life of the clinical microbiology laboratory. Microorganisms. 2024 Feb; 12(2): 322.
https://doi.org/10.3390/microorganisms12020322 - Candela A, Arroyo MJ, Sánchez-Molleda Á, et al. Rapid and reproducible MALDI-TOF-based method for the detection of vancomycin-resistant Enterococcus faecium using classifying algorithms. Diagnostics. 2022 Jan; 12(2): 328.
https://doi.org/10.3390/diagnostics12020328 - Cordovana M, Pranada AB, Ambretti S, Kostrzewa M. MALDI-TOF bacterial subtyping to detect antibiotic resistance. Clin Mass Spectr. 2019 Sept; 14: 3–8.
https://doi.org/10.1016/j.clinms.2019.06.002 - Elbehiry A, Aldubaib M, Abalkhail A, et al. How MALDI-TOF mass spectrometry technology contributes to microbial infection control in healthcare settings. Vaccines. 2022 Nov; 10(11): 1881.
https://doi.org/10.3390/vaccines10111881 - Elbehiry A, Abalkhail A. Spectral precision: recent advances in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for pathogen detection and resistance profiling. Microorganisms. 2025 Jun; 13(7): 1473.
https://doi.org/10.3390/microorganisms13071473 - Flores-Treviño S, Garza-González E, Mendoza-Olazarán S, et al. Screening of biomarkers of drug resistance or virulence in ESCAPE pathogens by MALDI-TOF mass spectrometry. Sci Rep. 2019 Dec; 9(1): 18945.
https://doi.org/10.1038/s41598-019-55430-1 - Florio W, Baldeschi L, Rizzato C, Tavanti A, Ghelardi E, Lupetti A. Detection of antibiotic-resistance by MALDI-TOF mass spec-trometry: an expanding area. Front Cell Infect Microbiol. 2020 Nov; 10: 572909.
https://doi.org/10.3389/fcimb.2020.572909 - Florio W, Tavanti A, Barnini S, Ghelardi E, Lupetti A. Recent advances and ongoing challenges in the diagnosis of microbial infections by MALDI-TOF mass spectrometry. Front Microbiol. 2018 May; 9: 1097.
https://doi.org/10.3389/fmicb.2018.01097 - Furniss RCD, Dortet L, Bolland W, et al. Detection of colistin resistance in Escherichia coli by use of the MALDI Biotyper Sirius mass spectrometry system. J Clinical Microbiol. 2019 Nov; 57(12): e01427–19.
https://doi.org/10.1128/JCM.01427-19 - Gajic I, Kabic J, Kekic D, et al. Antimicrobial susceptibility testing: a comprehensive review of currently used methods. Antibiotics. 2022 Mar; 11(4): 427.
https://doi.org/10.3390/antibiotics11040427 - Hosoda T, Suzuki M, Matsuno T, Matsui K, Ohyama K, Doi Y. Limitations of MALDI-TOF MS in identifying anaerobic bacteremia: challenges in polymicrobial infections and the role of whole-genome sequencing. Microbiol Spectr. 2025 Jun; 13(8): e0101425.
https://doi.org/10.1128/spectrum.01014-25 - Idelevich EA, Sparbier K, Kostrzewa M, Becker K. Rapid detection of antibiotic resistance by MALDI-TOF mass spectrometry using a novel direct-on-target microdroplet growth assay. Clin Microbiol Infect. 2018 Jul; 24(7): 738–43.
https://doi.org/10.1016/j. cmi.2017.10.016 - Idelevich EA, Nix ID, Busch JA, et al. Rapid simultaneous testing of multiple antibiotics by the MALDI-TOF MS direct-on-target microdroplet growth assay. Diagnostics. 2021 Sep; 11(10): 1803.
https://doi.org/10.3390/diagnostics11101803 - Idelevich EA, Storck LM, Sparbier K, Drews O, Kostrzewa M, Becker K. Rapid direct susceptibility testing from positive blood cultures by the matrix-assisted laser desorption ionization–time of flight mass spectrometry-based direct-on-target microdroplet growth assay. J Clinic Microbiol. 2018 Sept; 56(10): e00913–18.
https://doi.org/10.1128/JCM.00913-18 - Jeannot K, Hagart K, Dortet L, et al. Detection of colistin resistance in Pseudomonas aeruginosa using the MALDIxin test on the routine MALDI Biotyper Sirius mass spectrometer. Front Microbiol. 2021 Aug; 12: 725383.
https://doi.org/10.3389/fmicb.2021.725383 - Johansson Å, Nagy E, Sóki J. Detection of carbapenemase activities of Bacteroides fragilis strains with matrix-assisted laser desorption ionization – time of flight mass spectrometry (MALDI-TOF MS). Anaerobe. 2014 Apr; 26: 49–52.
https://doi.org/10.1016/j.anaerobe.2014.01.006 - Jung JS, Eberl T, Sparbier K, et al. Rapid detection of antibiotic resistance based on mass spectrometry and stable isotopes. Eur J Clin Microbiol Infect Dis. 2014; 33(6): 949–55.
https://doi.org/10.1007/s10096-013-2031-5 - Oviaño M, Rodríguez-Sánchez B. MALDI-TOF mass spectrometry in the 21st century clinical microbiology laboratory. Enferm Infecc Microbiol Clín. 2021 Apr; 39(4): 192–200.
https://doi.org/10.1016/j.eimc.2020.02.027 - Rubens R, Arruda I, Almeida R, Nóbrega Y, Carneiro M, Dalmolin T. Challenges in the detection of polymyxin resistance: from today to the future. Microorganisms, 2024 Jan; 12(1): 101.
https://doi.org/10.3390/microorganisms12010101 - Rychert J. Benefits and limitations of MALDI-TOF mass spectrometry for the identification of microorganisms. Journal of Infectiology, 2019 Jul; 2(4): 1–5.
https://doi.org/10.29245/2689-9981/2019/4.1142 - Santos P, Alho I, Ribeiro E. MALDI-TOF MS biomarkers for methicillin-resistant Staphylococcus aureus detection: a systematic review. Metabolites, 2025 Aug; 15(8): 540.
https://doi.org/10.3390/metabo15080540 - Sauget M, Bertrand X, Hocquet D. Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS. PLOS ONE, 2018 Oct; 13(10): e0205603.
https://doi.org/10.1371/journal.pone.0205603 - Sogawa K, Kiyosuke M, Hattori K, et al. Examination of conditions for regular internal quality control in identification of micro-organisms using MALDI-TOF MS. Biocontrol Sci. 2021 Jun; 26(2): 99–104.
https://doi.org/10.4265/bio.26.99 - Sorensen M, Chandler CE, Gardner FM, et al. Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids. Sci Rep. 2020 Dec; 10(1): 21536.
https://doi.org/10.1038/s41598-020-78401-3 - Sparbier K, Lange Ch, Jung J, Wieser A, Schubert S, Kostrzewa M. MALDI Biotyper-based rapid resistance detection by stable-isotope labeling. J Clin Microbiol. 2013 Nov; 51(11): 3741–48.
https://doi.org/10.1128/JCM.01536-13 - Tejan N, Fatima N, Yaduvanshi N, et al. Evaluation of direct microbial identification by MALDI-TOF MS and antimicrobial susceptibility testing for early diagnosis of blood stream infections. BMC Microbiol. 2025 Nov; 25(1): 724.
https://doi.org/10.1186/s12866-025-04401-w - Lin HTV, Yang TW, Lu WJ, Chiang HJ, Hsu PH. Machine learning-enhanced MALDI-TOF MS for real-time detection of antibiotic-resistant E. coli in food processing. LWT, 2025 May; 224: 117860.
https://doi.org/10.1016/j.lwt.2025.117860 - Vrioni G, Tsiamis C, Oikonomidis G, Theodoridou K, Kapsimali V, Tsakris A. MALDI-TOF mass spectrometry technology for detecting biomarkers of antimicrobial resistance: current achievements and future perspectives. Ann Transl Med. 2018 Jun; 6(12): 240–240.
https://doi.org/10.21037/atm.2018.06.28 - Weis C, Cuénod A, Rieck B, et al. Direct antimicrobial resistance prediction from clinical MALDI-TOF mass spectra using machine learning. Nat Med. 2022 Jan; 28(1): 164–74.
https://doi.org/10.1038/s41591-021-01619-9 - Yoon EJ, Jeong SH. MALDI-TOF mass spectrometry technology as a tool for the rapid diagnosis of antimicrobial resistance in bacteria. Antibiotics, 2021 Aug; 10(8): 982.
https://doi.org/10.3390/ antibiotics10080982