Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran

Abdolkhani, Abbas; Azimi, Sedighe

doi:10.21307/jofnem-2021-092

Abstract

During a survey on the biodiversity of plant-parasitic nematodes in Khuzestan province (southwest Iran), Bitylenchus hispaniensis was discovered around the rhizosphere of the euphrates poplar tree. The morphological and morphometric data were provided for the recovered species. To the best of our knowledge, this is the first report of B. hispaniensis from Iran and for the first time in association with euphrates poplar worldwide. Molecular phylogenetic analyses of the Iranian population of B. hispaniensis using the D2-D3 expansion segments of 28S rDNA and internal transcribed spacer (ITS rDNA) sequences using Bayesian inference (BI), showed a maximally supported clade with other sequences of the species.

References

Allen, M. W. 1955. A review of the nematode genus Tylenchorhynchus. University of California Publications in Zoology 61:129–166.
Search in Google Scholar Back to article
Andrássy, I.2007. Free-living nematodes of Hungary (Nematoda errantia). Vol. II. Csuzdi, C. and Mahunka, S. Budapest, Hungary: Hungarian Natural History Museum and Systematic Zoology Research Group of the Hungarian Academy of Sciences.
Search in Google Scholar Back to article
Bütschli, O. 1873. Beiträge zur Kennntis der freilebenden Nematoden. Beiträge zur Kenntnis der freilebenden Nematoden. Nova acta Academiae Caesareae Leopoldino-Carolinae Germanicae Naturae Curiosorum 36:1–124.
Search in Google Scholar Back to article
Cobb, N. A. 1913. New nematode genera found inhabiting fresh water and nonbrackish soils. Journal of the Washington Academy of Sciences 3:432–444.
Search in Google Scholar Back to article
De Grisse, A. T. 1969. Redescription ou modification de quelques techniques utilissée dans l’étude des nematodes phytoparasitaires. Mededelingen Rijksfaculteit Landbouwwetenschappen Gent 34:351–369.
Search in Google Scholar Back to article
Filipjev, I. N. 1934. The classification of the free-living nematodes and their relation to the parasitic nematodes. Smithsonian Miscellaneous Collections 89:1–63.
Search in Google Scholar Back to article
Fortuner, R. and Luc, M. 1987. A reappraisal of Tylenchina (Nemata). 6. The family Belonolaimidae Whitehead, 1960. Revue de Nématologie 10:183–202.
Search in Google Scholar Back to article
Fuchs, A. G. 1937. Neue parasitische und halbparasitische Nematoden bei Borkenkäfern und einige andere Nematoden. I. Teil. Zoologische Jahrbücher, Abteilung für Systematik, Ökologie und Geographie der Tiere 70:291–380.
Search in Google Scholar Back to article
Geraert, E.2011. The Dolichodoridae of the world. Identification of the family Dolichodoridae. Ghent, Belgium: Academia Press.
Search in Google Scholar Back to article
Gómez Barcina, A., Siddiqi, M. R. and Castillo, P. 1992. Bitylenchus Filipjev, 1934 (Nematoda: Tylenchida) with descriptions of two new species from Spain. Journal of Helminthological Society of Washington 59:96–100.
Search in Google Scholar Back to article
Handoo, Z. A. 2000. A key and diagnostic compendium to the species of the genus Tylenchorhynchus Cobb, 1913 (Nematoda: Belonolaimidae). Journal of Nematology 32:20–34.
Search in Google Scholar Back to article
Handoo, Z. A., Palomares-Rius, J. E., Cantalapiedra-Navarrete, C., Liébanas, G., Subbotin, S. A. and Castillo, P. 2014. Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny. Zoological Journal of the Linnean Society 172:231–264.
Search in Google Scholar Back to article
Hosseinvand, M., Eskandari, A., Ganjkhanloo, S., Ghaderi, R., Castillo, P. and Palomares-Rius, J. E. 2020. Taxonomical considerations and molecular phylogeny of the closely related genera Bitylenchus, Sauertylenchus and Tylenchorhynchus (Nematoda: Telotylenchinae), with one new and four known species from Iran. Journal of Helminthology 94:1–25.
Search in Google Scholar Back to article
Huson, D. H. and Scornavacca, C. 2012. Dendroscope 3: An interactive tool for rooted phylogenetic trees and networks. Systematic Biology 61:1061–1067.
Search in Google Scholar Back to article
Jairajpuri, M. S. 1982. Some studies on Tylenchorhynchinae: the subgenus Bitylenchus Filipjev, 1934, with description of Tylenchorhynchus (Bitylenchus) depressus n. sp. and a key to species of Bitylenchus. Mededelingen van der Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 47:765–770.
Search in Google Scholar Back to article
Jenkins, W. R. 1964. A rapid centrifugal flotation technique for separating nematodes from soil. Plant Disease Reporter 48:692.
Search in Google Scholar Back to article
Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33:1870–1874.
Search in Google Scholar Back to article
Larget, B. and Simon, D. L. 1999. Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16:750–759.
Search in Google Scholar Back to article
Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J. and Higgins, D. G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948.
Search in Google Scholar Back to article
Nunn, G. B.1992. Nematode molecular evolution PhD Thesis. University of Nottingham, UKoxfordjournals.molbev.a026160.
Search in Google Scholar Back to article
Nylander, J. A. A.2004. MrModeltest v2. Evolutionary Biology Centre. Uppsala University, Sweden.
Search in Google Scholar Back to article
Örley, L. 1880. Az anguillulidak maganrajza. A kir.m. termeszettudom. Tersulat altal a bugatdijjal jutalmazott palyamii. Termeszetr. Fuz 4:16–50.
Search in Google Scholar Back to article
Paetzold, D. 1958. Beitrage zur Nematodenfauna mitteldeutscher Salzstellen im Raum von Halle. Wissenschaftliches Zeitschrift Universitiit Hall, Mathematisch-naturwissenschaftliche Reihe 8:17–48.
Search in Google Scholar Back to article
Ronquist, F. and Huelsenbeck, J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574.
Search in Google Scholar Back to article
Shokoohi, E. 2021. First report of Bitylenchus ventrosignatus (Tobar-Jiménez, 1969) Siddiqi, 1986 associated with wild grass in Botswana. Journal of Nematology 53:1–9.
Search in Google Scholar Back to article
Siddiqi, M. R.1986. Tylenchida parasites of plants and insects. Commonwealth Agricultural Bureaux, Slough.
Search in Google Scholar Back to article
Siddiqi, M. R.2000. Tylenchida: parasites of plants and insects. CABI Publishing, Wallingford.
Search in Google Scholar Back to article
Subbotin, S. A., Halford, P. D., Warry, A. and Perry, R. N. 2000. Variations in ribosomal DNA sequences and phylogeny of Globodera parasiting solanaceous plants. Nematology 2:591–604.
Search in Google Scholar Back to article
Thorne, G. 1949. On the classification of the Tylenchida, new order (Nematoda: Phasmida). Proceedings of the Helminthological Society of Washington 16:37–73.
Search in Google Scholar Back to article
Tobar-Jiménez, A. 1969. Descripción del Tylenchorhynchus ventrosignatus sp. n. (Nematoda: Tylenchida). Revista Iberica de Parasitología 29:399–403.
Search in Google Scholar Back to article
Tzortzakakis, E. M., Cantalapiedra-Navarrete, C., Kormpi, M., Lazanaki, M. S., Castillo, P. and Archidona-Yuste, A. 2018. First report of Bitylenchus hispaniensis, Pratylenchoides alkani, and Helicotylenchus vulgaris in association with cultivated and wild olives in Crete, Greece and molecular identification of Helicotylenchus microlobus and Merlinius brevidens. Journal of Nematology 50:413–418.
Search in Google Scholar Back to article
Whitehead, A. G. and Hemming, J. R. 1965. A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology 55:25–38.
Search in Google Scholar Back to article

Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran

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