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Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran Cover

Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran

Open Access
|Nov 2021

Figures & Tables

Figure 1:

Line drawings of Bitylenchus hispaniensis from Iran. A-F: Female. A: Anterior body region; B: Lateral field at mid-body; C: Part of reproductive system; D-F: Posterior body region; G, H: Posterior body region of male.
Line drawings of Bitylenchus hispaniensis from Iran. A-F: Female. A: Anterior body region; B: Lateral field at mid-body; C: Part of reproductive system; D-F: Posterior body region; G, H: Posterior body region of male.

Figure 2:

Light photomicrographs of Bitylenchus hispaniensis from Iran. A, C-M: Female. A: Entire body; C, D: Anterior body region; E-G: Pharyngeal region; H: Lateral field at mid-body; I, J: Vulval region; K-M: Posterior body region (the arrow indicates the phasmid); B, N-P: Male. B: Entire body; N-P: Posterior body region. (Scale bars: A, B = 50 μm; C-P = 10 μm).
Light photomicrographs of Bitylenchus hispaniensis from Iran. A, C-M: Female. A: Entire body; C, D: Anterior body region; E-G: Pharyngeal region; H: Lateral field at mid-body; I, J: Vulval region; K-M: Posterior body region (the arrow indicates the phasmid); B, N-P: Male. B: Entire body; N-P: Posterior body region. (Scale bars: A, B = 50 μm; C-P = 10 μm).

Figure 3:

Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.

Figure 4:

Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rRNA gene of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequence is indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rRNA gene of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequence is indicated in bold.

Morphometrics of Bitylenchus hispaniensis from Khuzestan province, Iran and compare with the original description and Greek population_

Khuzestan province, Iran Handoo et al. (2014) Córdoba, Córdoba, Spain Tzortzakakis et al. (2018) Crete, Greece
CharacterFemalesMalesFemalesMalesFemales
n1497103
L622.1 ± 41.0 (570–704)661.7 ± 58.0 (591–763)662 ± 38.4 (612–732)640 ± 60.9 (548–760)722 ± 47 (670–760)
a25.6 ± 3.5 (21.8–2.8)29.5 ± 0.1 (27.6–32.8)30.4 ± 1.7 (27.8–32.5)31.0 ± 3.9 (27.0–39.1)32.3 ± 0.6 (31.9–33.0)
b5.5 ± 0.4 (5.0–6.3)5.9 ± 0.4 (5.3–6.5)6.0 ± 0.4 (5.6–6.8)6.1 ± 0.7 (5.4–7.8)6.3 ± 0.4 (6.1–6.8)
c13.3 ± 0.7 (12.8–15.7)14.6 ± 0.7 (13.5–15.7)14.2 ± 1.7 (11.8–16.8)17.7 ± 1.4 (15.2–20.7)14.9 ± 0.6 (14.4–15.5)
c′2.8 ± 0.4 (2.5–3.2)2.9 ± 0.2 (2.6–3.2)2.9 ± 0.3 (2.4–3.3)2.7 ± 0.2 (2.5–3.0)3.0 ± 0.2 (2.7–3.2)
V54.3 ± 1.5 (52.7–57.5)-54.6 ± 1.5 (52.5–57.0)-55.0 ± 1.0 (54–56)
Lip region height3.7 ± 0.9 (3.2–4.1)3.4 ± 0.3 (3.2–4.1)3.6 ± 0.3 (3–4)--
Lip region width6.7 ± 0.2 (6.5–7.0)6.4 ± 0.1 (6.0–6.5)7.2 ± 0.3 (6.5–7.5)--
Stylet length17.5 ± 0.3 (17–18)17.1 ± 0.4 (16.2–17.5)16.1 ± 0.5 (15.5–17.0)15.9 ± 0.8 (15.0–18.0)17.3 ± 0.3 (17.0–17.5)
m58.0 ± 2.7 (55.7–63.1)54.2 ± 2.7 (48.2–57.3)---
DGO2.9 ± 0.2 (2.6–3.0)2.8 ± 0.2 (2.5–3.0)2.5--
MB49.2 ± 2.1 (46.8–52.6)51.2 ± 4.0 (45.2–57.3)---
Maximum body width24.7 ± 7.4 (20.8–29.2)20.8 ± 1.3 (18.8–23.4)21.8 ± 1.0 (20.5–23.5)20.7 ± 1.3 (18.0–23.0)22.3 ± 1.2 (21–23)
Pharynx length115.3 ± 6.7 (105–120)116.0 ± 6.8 (106–124)110.0 ± 6.5 (100–120)105.4 ± 8.2 (90–120)114.0 ± 5.3 (110–120)
Anterior end to median bulb56.7 ± 3.8 (50.0–61.7)56.9 ± 2.8 (52–58)---
Anterior end to nerve ring77.8 ± 2.9 (73.0–84.5)80.0 ± 4.2 (75.0–87.7)83.1 ± 5.6 (73–95)--
Anterior end to excretory pore93.3 ± 6.1 (85.5–104.0)87.6 ± 9.0 (87–104)89.7 ± 3.9 (85–95)--
Body width at vulva24.5 ± 2.7 (21.5–27.9)-- -
Anal body width16.5 ± 1.5 (15.0–19.5)15.4 ± 1.5 (13.6–17.5)16.1 ± 1.4 (13.5–17.5)12.8 ± 0.8 (12–15)16.5 ± 2.2 (14–18)
Tail length47.4 ± 6.5 (37.0–55.2)43.9 ± 3.5 (39.5–48.0)47.4 ± 6.3 (37.5–57.0)36.6 ± 5.6 (30–50)48.3 ± 3.1 (45–51)
Tail annuli51.8 ± 5.3 (40–58)-55.1 ± 8.1 (40–66)--
Spicule length-25.5 ± 0.8 (24.0–26.5)-25.3 ± 1.8 (23–30)-
Gubernaculum length-12.5 ± 0.4 (12.0–13.3)-11.4 ± 1.6 (10–15)-
DOI: https://doi.org/10.21307/jofnem-2021-092 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 10
Published on: Nov 3, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2021 Abbas Abdolkhani, Sedighe Azimi, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.