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Characterization of a population of Pelodera strongyloides (Nematoda: Rhabditidae) associated with the beetle Lucanus ibericus (Coleoptera: Lucanidae) from Georgia Cover

Characterization of a population of Pelodera strongyloides (Nematoda: Rhabditidae) associated with the beetle Lucanus ibericus (Coleoptera: Lucanidae) from Georgia

Open Access
|Aug 2020

Figures & Tables

Figure 1:

Pelodera strongyloides – Female. A: En face view; B: Anterior region (lateral); C: Pharyngeal bulb (lateral); D: Tail region; E: T region in ventral view; F: Vulval region in lateral view (sphincter, uterus, eggs). Male. G: Bursa (ventral); H: Tail (lateral); I: Aberrations of spicules, (ventral); J: Gubernaculum (ventral); K: Bursa (lateroventral). Scales (in μm): A, B = 10; C, D, E, F, I, J, H, K = 22.5.
Pelodera strongyloides – Female. A: En face view; B: Anterior region (lateral); C: Pharyngeal bulb (lateral); D: Tail region; E: T region in ventral view; F: Vulval region in lateral view (sphincter, uterus, eggs). Male. G: Bursa (ventral); H: Tail (lateral); I: Aberrations of spicules, (ventral); J: Gubernaculum (ventral); K: Bursa (lateroventral). Scales (in μm): A, B = 10; C, D, E, F, I, J, H, K = 22.5.

Figure 2:

Pelodera strongyloides – Dauer larva. A: Anterior region, lateral; B: Pharyngeal basal bulb; C: Caudal region, in lateral view; third juvenile stage. D: Anterior region, in ventral view; E: Pharyngeal basal bulb; F: Caudal region, in ventral view. Scale bar = 10 μm.
Pelodera strongyloides – Dauer larva. A: Anterior region, lateral; B: Pharyngeal basal bulb; C: Caudal region, in lateral view; third juvenile stage. D: Anterior region, in ventral view; E: Pharyngeal basal bulb; F: Caudal region, in ventral view. Scale bar = 10 μm.

Figure 3:

Pelodera strongyloides light photomicrographs – Female. A: Anterior end, lateral; B: Pharyngeal basal bulb; C: Vulval region, in lateral view; D: Tail region; E: Entire female; F: Pharyngeal region; Male. G: Tail, in lateral view; H: Tail, in ventral view; I: Excised spicules with gubernaculum; J, K: Tail, in lateroventral view; L: Cells of the spermatheca. Scales (in μm): A, B, I, K, L = 16; C, F, H = 50; E = 250; D, G, J = 22.5.
Pelodera strongyloides light photomicrographs – Female. A: Anterior end, lateral; B: Pharyngeal basal bulb; C: Vulval region, in lateral view; D: Tail region; E: Entire female; F: Pharyngeal region; Male. G: Tail, in lateral view; H: Tail, in ventral view; I: Excised spicules with gubernaculum; J, K: Tail, in lateroventral view; L: Cells of the spermatheca. Scales (in μm): A, B, I, K, L = 16; C, F, H = 50; E = 250; D, G, J = 22.5.

Figure 4:

Phylogenetic tree of D2 to D3 expansion domains of 28S rRNA gene describing the evolutionary relationships among different geographical populations of Georgian Pelodera strongyloides under the maximum likelihood criterion. Values at nodes indicate bootstrap support.
Phylogenetic tree of D2 to D3 expansion domains of 28S rRNA gene describing the evolutionary relationships among different geographical populations of Georgian Pelodera strongyloides under the maximum likelihood criterion. Values at nodes indicate bootstrap support.

Figure 5:

Restriction profiles of the ITS region of Georgian Pelodera strongyloides separated on 2.5% agarose gel. M: 100 bp DNA ladder; P) Pst I; D) Dde I; R) Rsa I; A) Alu I; H) Hinf I.
Restriction profiles of the ITS region of Georgian Pelodera strongyloides separated on 2.5% agarose gel. M: 100 bp DNA ladder; P) Pst I; D) Dde I; R) Rsa I; A) Alu I; H) Hinf I.

Data matrix for cluster analysis of the species of Pelodera ‘strongyloides’ groupa_

Characters123456789101112131415161718192021
P. cutanea 100110000200200011111
P. nidicolis 001110000210210000000
P. orbitalis 110001000200200010001
P. strongyloides 020011100210201110000
P. strongyloides ex Lucanus ibericus 020011100110000110000
P. termitis 000011?01101101120000
P. scrofulata 111110011001000010111

Ratios and measurements (in µm) of heat relaxed specimens of Pelodera strongyloides collected from Lucanus ibericus in Georgia_

CharacterFemalesMales3rd juvenile stage P. strongyloides (Schneider, 1866)
n 20202211
Body length1566  ±  120 (1353-1867)1280 ± 100 (1080-1494)663 ± 51 (566-772)1629 (1142-2149)
Body width96 ± 10 (75-11262 ± 4.8 (55-70)30 ± 2.4 (25-35)107 (80-132)
Lip region diam19 ± 1.6 (16-2217 ± 1.5 (15-20)
Stoma length31 ± 1 (30-32)29 ± 2.2 (25-32)24 ± 1.7 (22-30)33 (30-38)
Stoma diam5.1 ± 0.4 (5.0-6.2)4.7 ± 0.4 (3.7-5.0)2.6 ± 0.4 (2.5-3.7)
Anterior end to nerve ring178 ± 6 (172-197)155 ± 10 (137-175)102 ± 6 (92-120)
Stoma length as % pharynx length12.2 ± 0.4 (11-13)13 ± 1.2 (11-15)16 ± 0.8 (14-17)
Pharynx length252 ± 5 (237-257)219 ± 17 (192-250)152 ± 6 (142-177)
Corpus length144 ± 7 (135-162)122 ± 10 (107-137)87 ± 2 (80-92)
Corpus as % pharynx length56 ± 2.3 (53-63)55 ± 2 (50-59)57 ± 2.5 (52-61)
Median bulb (MB) diam27 ± 2.1 (22-30)30 ± 4.9 (20-37)11 ± 0.8 (10-12)
Terminal bulb (TB) diam39 ± 4.1 (32-47)34 ± 2.7 (32-40)15 ± 0.6 (12-17)36-50
MB diam. as % TB diam77 ± 9.5 (54-88)79 ± 6.8 (62-93)
Anterior end to excretory pore236 ± 14 (212-270)205 ± 13 (164-227)130  ± 10 (110-150)235 (183-295)
Ex. pore posit. as % phar. length93 ± 5 (83-105)93 ± 8 (75-109)85 ± 6.7 (75-98)
Tail length69 ± 11 (52-95)53 ± 5 (45-62)72 ± 4 (65-82)
Anal (cloacal) body width (ABW)40 ± 3 (32-47)37 ± 3 (32-42)19 ± 1.8 (16-22)
Gonad lengtha 1285 ± 198 (995-1658)956 ± 87 (747-1268)890 (653-1157)
Gonad length as % body length82 ± 15 (61-107)72 ± 5 (66-82)46-63
Gonad length. as % intestine leng.b 108 ± 23 (72-143)93 ± 11 (69-110 )
Anterior gonad branch length800 ± 104 (555-928)
Posterior branch length546 ± 128 (290-794)
Testis flexure length201 ± 49 (123-260)
Ant. flexure as % of ant. branch33 ± 3 (26-42)17 ± 4 (13-24)
Post. flex. as % of post. branch27 ± 6 (13-34)
Sperm diametern.d.5 ± 0.6 3. (7-7.5)
Egg length60 ± 3.4 (55-67)
Egg diam41 ± 4 (35-47)
Rectum length38 ± 3.2 (30-42)n.d.24 ± 1.5 (22-25)
Rectum length/ABW0.9 ± 0.1 (0.8-1.3)n.d.0.9 ± 0.1 (7-1.2)
Anus to phasmid distance23 ± 1.8 (20-27)n.d.
Anus to phasmid distance/ABW0.6 ± 0.05 (0.4-0.7)n.d.
Posit. phasmid as % tail length35 ± 6 (27-48)32-61
Spicule length66 ± 3 (60-72)55-82
Gubernaculum length44 ± 2.2 (40-50)
Gubern. leng. as % spic. length66 ± 4.5( 60-78)
a 16.3 ± 2 (13.5-20.3)20.7 ± 1 (19-23.1)22.3 ± 1.5 (19.3-25.8)15.1 (12-18.7)
b 6.1 ± 0.4 (5.4-7)5.8 ± 0.5 (5.1-7)4.2 ± 0.2 (3.7-4.7)6.2 (4.9-7.6)
c 23 ± 3.4 (17.6-32.2)24.3 ± 2.8 (20.5-30.4)9.1 ± 0.5 (8-10)33 (26.3-37.9)
c′ (= tail length/ABW)1.6 ± 0.6 (1.3-2.2)1.3 ± 0.1 (1.2-1.7)3.7 ± 0.3 (3.2-4.5)
V (vulva posit. in % body length)57 ± 1.9 (51.8-59.1)58 (55-64)
DOI: https://doi.org/10.21307/jofnem-2020-081 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 12
Published on: Aug 31, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 O. Gorgadze, A. Troccoli, E. Fanelli, E. Tarasco, F. De Luca, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.