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A new stunt nematode, Geocenamus chengi n. sp. (Nematoda: Merliniinae) in the rhizosphere of tea (Camellia sinensis) from Zhejiang Province, China

Open Access
|Apr 2020

Figures & Tables

Figure 1:

Line drawings of Geocenamus chengi n. sp. A: pharyngeal region; B, C: en face view; D: lateral lines: E: female gonad; F: male gonad; G: vulval region; H-J: female tails: K-M: male tails (Scale bars = A; D-M = 10 μm, B,C = 5 μm).
Line drawings of Geocenamus chengi n. sp. A: pharyngeal region; B, C: en face view; D: lateral lines: E: female gonad; F: male gonad; G: vulval region; H-J: female tails: K-M: male tails (Scale bars = A; D-M = 10 μm, B,C = 5 μm).

Figure 2:

Light photomicrographs of Geocenamus chengi n. sp. Female. A: entire body; B: lateral lines; C, D: pharyngeal regions, arrow pointing on the excretory pore (exp): E: gonad; F: vulval region arrows pointing on vulva (v) and spermatheca (sp); G, H: vulval region; I-K: female tails arrows pointing on anus (a) and phasmid (ph) (Scale bars = A = 100 μm; B-K = 10 μm).
Light photomicrographs of Geocenamus chengi n. sp. Female. A: entire body; B: lateral lines; C, D: pharyngeal regions, arrow pointing on the excretory pore (exp): E: gonad; F: vulval region arrows pointing on vulva (v) and spermatheca (sp); G, H: vulval region; I-K: female tails arrows pointing on anus (a) and phasmid (ph) (Scale bars = A = 100 μm; B-K = 10 μm).

Figure 3:

Light photomicrographs of Geocenamus chengi n. sp. Male. A: entire body; pharyngeal regions, arrow pointing on the excretory pore (exp); C: tail with bursa; D: gonad; E, F: tail in ventral view; G, I: male tail arrows pointing on phasmid (ph) (Scale bars = A = 100 μm; B-I = 10 μm).
Light photomicrographs of Geocenamus chengi n. sp. Male. A: entire body; pharyngeal regions, arrow pointing on the excretory pore (exp); C: tail with bursa; D: gonad; E, F: tail in ventral view; G, I: male tail arrows pointing on phasmid (ph) (Scale bars = A = 100 μm; B-I = 10 μm).

Figure 4:

Scanning electron microscopy of Geocenamus chengi n. sp. A: anterior region; B-C: en face view; D-F: vulval regions; G, H: female tail regions arrows pointing anus (a); I: anal area at higher resolution, arrow pointing on the position of anus; J: cloacal area at higher resolution, arrow pointing on the position of cloaca (c); K, L: male tail; M: lateral field with phasmid (ph); N: lateral field. Abbreviation: am, apmphid; ds, dorsal sectors; vs, =ventral sectors; ld, labial disc (Scale bars, A, E, I, J, M, N = 10 μm; B, C, D, E, F = 5 μm; G, K = 30 μm; H = 50 μm; L = 20 μm).
Scanning electron microscopy of Geocenamus chengi n. sp. A: anterior region; B-C: en face view; D-F: vulval regions; G, H: female tail regions arrows pointing anus (a); I: anal area at higher resolution, arrow pointing on the position of anus; J: cloacal area at higher resolution, arrow pointing on the position of cloaca (c); K, L: male tail; M: lateral field with phasmid (ph); N: lateral field. Abbreviation: am, apmphid; ds, dorsal sectors; vs, =ventral sectors; ld, labial disc (Scale bars, A, E, I, J, M, N = 10 μm; B, C, D, E, F = 5 μm; G, K = 30 μm; H = 50 μm; L = 20 μm).

Figure 5:

Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the 18 S rRNA gene sequence data set with the TIM3ef + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the 18 S rRNA gene sequence data set with the TIM3ef + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 6:

Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence data set with the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence data set with the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 7:

Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the ITS rRNA gene sequence data set with the TIM2 + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the ITS rRNA gene sequence data set with the TIM2 + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Morphometric data for Geocenamus chengi n_ sp_ All measurements are in µm and in the form of mean ± SD (range)_

HolotypeParatype
FemaleMale
n 2010
Body length884.8879.0 ± 59.6 (783–997)822.8 ± 34.2 (772–892)
a33.333.3 ± 2.6 (28–39)32.9 ± 1.2 (31–34)
b6.06.1 ± 0.3 (5.5–6.6)6.1 ± 0.2 (5.8–6.6)
c10.410.5 ± 0.6 (9.2–11.7)9.7 ± 0.6 (8.7–10.3)
c'4.74.6 ± 0.4 (4.2–5.5)4.8 ± 0.3 (4.5–5.2)
V52.852.5 ± 1.6 (49.5–55.7)39.4 ± 5.4 (33.1–47.4)
Lip height7.57.6 ± 2.4 (7.0–8.0)7.6 ± 0.3 (7.0–8.0)
Lip width3.73.8 ± 0.3 (3.4–4.5)3.7 ± 0.3 (3.2–4.0)
Stylet length22.322.2 ± 0.6 (21–23)21.5 ± 0.7 (20–23)
DGO distance from stylet knobs2.02.0 ± 0.1 (1.7–2.2)2.1 ± 0.1 (1.9–2.3)
Excretory pore from anterior end122.8120.6 ± 5.2 (109–126)108.3 ± 5.2 (101–116)
Hemizonid from anterior end118.4116.7 ± 5.1 (104–123)103.0 ± 6.7 (94–113)
Pharynx length148.4145 ± 5.7 (130–152)134 ± 3.6 (127–139)
Maximum body diam.26.626.6 ± 2.5 (22–33)25.0 ± 1.0 (23–27)
Vulval body diam.25.426.6 ± 2.3 (23–32)
Anal/cloacal body diam.18.318.1 ± 1.2 (16–21)17.7 ± 0.9 (16–19)
Tail length85.283.8 ± 5.4 (76–92)85.2 ± 5.4 (78–93)
Hyaline tail part length25.222.4 ± 2.2 (19–25)21.3 ± 1.9 (18–24)
Phasmid position from anus/cloaca12.415.5 ± 4.1 (10–19)24.6 ± 3.9 (19–29)
Phasmid % of tail14.617.4 ± 4.0 (12–20)29.5 ± 4.0 (24–35)
Spicule length 23.4 ± 1.9 (22–25)
Gubernaculum 8.0 ± 0.7 (7–9)
DOI: https://doi.org/10.21307/jofnem-2020-042 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 13
Submitted on: Feb 2, 2020
Published on: Apr 16, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Munawar Maria, Wentao Miao, Pablo Castillo, Jingwu Zheng, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.