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A new stunt nematode, Geocenamus chengi n. sp. (Nematoda: Merliniinae) in the rhizosphere of tea (Camellia sinensis) from Zhejiang Province, China Cover

A new stunt nematode, Geocenamus chengi n. sp. (Nematoda: Merliniinae) in the rhizosphere of tea (Camellia sinensis) from Zhejiang Province, China

Open Access
|Apr 2020

Figures & Tables

Figure 1:

Line drawings of Geocenamus chengi n. sp. A: pharyngeal region; B, C: en face view; D: lateral lines: E: female gonad; F: male gonad; G: vulval region; H-J: female tails: K-M: male tails (Scale bars = A; D-M = 10 μm, B,C = 5 μm).

Figure 2:

Light photomicrographs of Geocenamus chengi n. sp. Female. A: entire body; B: lateral lines; C, D: pharyngeal regions, arrow pointing on the excretory pore (exp): E: gonad; F: vulval region arrows pointing on vulva (v) and spermatheca (sp); G, H: vulval region; I-K: female tails arrows pointing on anus (a) and phasmid (ph) (Scale bars = A = 100 μm; B-K = 10 μm).

Figure 3:

Light photomicrographs of Geocenamus chengi n. sp. Male. A: entire body; pharyngeal regions, arrow pointing on the excretory pore (exp); C: tail with bursa; D: gonad; E, F: tail in ventral view; G, I: male tail arrows pointing on phasmid (ph) (Scale bars = A = 100 μm; B-I = 10 μm).

Figure 4:

Scanning electron microscopy of Geocenamus chengi n. sp. A: anterior region; B-C: en face view; D-F: vulval regions; G, H: female tail regions arrows pointing anus (a); I: anal area at higher resolution, arrow pointing on the position of anus; J: cloacal area at higher resolution, arrow pointing on the position of cloaca (c); K, L: male tail; M: lateral field with phasmid (ph); N: lateral field. Abbreviation: am, apmphid; ds, dorsal sectors; vs, =ventral sectors; ld, labial disc (Scale bars, A, E, I, J, M, N = 10 μm; B, C, D, E, F = 5 μm; G, K = 30 μm; H = 50 μm; L = 20 μm).

Figure 5:

Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the 18 S rRNA gene sequence data set with the TIM3ef + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 6:

Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence data set with the GTR + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 7:

Phylogenetic relationships of Geocenamus chengi n. sp. with other merlinids as inferred from Bayesian analysis using the ITS rRNA gene sequence data set with the TIM2 + I + G model. Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Morphometric data for Geocenamus chengi n_ sp_ All measurements are in µm and in the form of mean ± SD (range)_

HolotypeParatype
FemaleMale
n 2010
Body length884.8879.0 ± 59.6 (783–997)822.8 ± 34.2 (772–892)
a33.333.3 ± 2.6 (28–39)32.9 ± 1.2 (31–34)
b6.06.1 ± 0.3 (5.5–6.6)6.1 ± 0.2 (5.8–6.6)
c10.410.5 ± 0.6 (9.2–11.7)9.7 ± 0.6 (8.7–10.3)
c'4.74.6 ± 0.4 (4.2–5.5)4.8 ± 0.3 (4.5–5.2)
V52.852.5 ± 1.6 (49.5–55.7)39.4 ± 5.4 (33.1–47.4)
Lip height7.57.6 ± 2.4 (7.0–8.0)7.6 ± 0.3 (7.0–8.0)
Lip width3.73.8 ± 0.3 (3.4–4.5)3.7 ± 0.3 (3.2–4.0)
Stylet length22.322.2 ± 0.6 (21–23)21.5 ± 0.7 (20–23)
DGO distance from stylet knobs2.02.0 ± 0.1 (1.7–2.2)2.1 ± 0.1 (1.9–2.3)
Excretory pore from anterior end122.8120.6 ± 5.2 (109–126)108.3 ± 5.2 (101–116)
Hemizonid from anterior end118.4116.7 ± 5.1 (104–123)103.0 ± 6.7 (94–113)
Pharynx length148.4145 ± 5.7 (130–152)134 ± 3.6 (127–139)
Maximum body diam.26.626.6 ± 2.5 (22–33)25.0 ± 1.0 (23–27)
Vulval body diam.25.426.6 ± 2.3 (23–32)
Anal/cloacal body diam.18.318.1 ± 1.2 (16–21)17.7 ± 0.9 (16–19)
Tail length85.283.8 ± 5.4 (76–92)85.2 ± 5.4 (78–93)
Hyaline tail part length25.222.4 ± 2.2 (19–25)21.3 ± 1.9 (18–24)
Phasmid position from anus/cloaca12.415.5 ± 4.1 (10–19)24.6 ± 3.9 (19–29)
Phasmid % of tail14.617.4 ± 4.0 (12–20)29.5 ± 4.0 (24–35)
Spicule length 23.4 ± 1.9 (22–25)
Gubernaculum 8.0 ± 0.7 (7–9)
DOI: https://doi.org/10.21307/jofnem-2020-042 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 13
Submitted on: Feb 2, 2020
Published on: Apr 16, 2020
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Munawar Maria, Wentao Miao, Pablo Castillo, Jingwu Zheng, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.