Table 1.
Morphometric characters of Coslenchus paramaritus n. sp. and C. cancellatus from Iran (measurements are in µm).
| C. paramaritus n. sp. | C. cancellatus | ||||||
|---|---|---|---|---|---|---|---|
| Character\Species | Holotype | Females | Males | Females | |||
| n | – | 10 | CV | 8 | CV | 12 | CV |
| L | 519 | 537 ± 22.3 (508–572) | 4.2 | 529 ± 31.4 (474–571) | 5.9 | 446 ± 29.9 (388–481) | 6.7 |
| a | 26.6 | 27.3 ± 3 (22.5–33.6) | 11.1 | 36 ± 1.6 (33.7–38.2) | 4.6 | 25.6 ± 1.8 (23.2–28.1) | 7.3 |
| b | 5.9 | 5.4 ± 0.3 (5.1–6.1) | 5.9 | 14.8 ± 0.4 (14–15.3) | 2.9 | 4.7 ± 0.4 (3.4–5.1) | 10.6 |
| c | 4.5 | 4.7 ± 0.4 (4.3–5.7) | 9.6 | 4.6 ± 0.1 (4.3–4.9) | 4.0 | 5.8 ± 0.5 (4.9–6.5) | 9.1 |
| c′ | 11.9 | 10.7 ± 1.2 (7.8–11.8) | 11.5 | 11.3 ± 0.9 (10.1–13) | 8.6 | 7.5 ± 0.7 (6.5–8.4) | 9.6 |
| V or T | 62.6 | 63.8 ± 2.3 (61.8–69.6) | 3.7 | 34 ± 4.1 (28.6–39.4) | 12.1 | 66.4 ± 1.5 (64.6–69.9) | 2.3 |
| V' | 80.4 | 80.5 ± 1.9 (76.7–83.2) | 2.4 | – | – | 80.2 ± 1.8 (77–82.9) | 2.3 |
| Stylet | 13.5 | 12.8 ± 0.6 (12–13.9) | 5.2 | 12.4 ± 0.2 (12–12.9) | 2.3 | 10.9 ± 0.3 (10.5–11.4) | 2.8 |
| m (conus/stylet %) | 45.3 | 46.1 ± 1.8 (43.2–48.3) | 3.9 | 47.8 ± 1.3 (45.8–49.5) | 2.7 | 44.8 ± 2 (42.2–48.5) | 4.7 |
| Pharynx | 90 | 97.9 ± 5.8 (85–107) | 6.0 | 97.1 ± 4.4 (92–103) | 4.6 | 95.4 ± 9 (85–112) | 9.5 |
| Median bulb | 44.5 | 44.6 ± 2.1 (41–47) | 4.9 | 44.6 ± 2.2 (42–49) | 5.1 | 41.9 ± 3.8 (38–50) | 9.3 |
| MB | 47 | 45.6 ± 2.5 (42.4–51.7) | 5.6 | 45.9 ± 1.1 (44.5–47.5) | 2.4 | 43.9 ± 1.4 (41.8–46.6) | 3.2 |
| Excretory pore | 79 | 81 ± 3.3 (76–86) | 4.2 | 79.9 ± 3 (74–83) | 3.8 | 75.6 ± 12 (59–103) | 15.9 |
| Head-vulva | 325 | 340 ± 17.6 (314–370) | 5.2 | – | – | 296 ± 18.2 (257–319) | 6.2 |
| Head-anus | 404 | 423 ± 22.7 (394–459) | 5.4 | 414 ± 24.1 (373–442) | 5.8 | 369 ± 28 (310–401) | 7.6 |
| Vulva–anus | 89 | 82.6 ± 10.5 (71–104) | 12.8 | – | – | 73 ± 11.5 (53–92) | 15.8 |
| Body width | 19 | 19.6 ± 1.6 (17–22) | 8.6 | 14.7 ± 1.1 (12.5–15.8) | 7.5 | 17.4 ± 2.1 (14.2–20.7) | 12.5 |
| Vulval body width | 16 | 17.1 ± 1 (15.5–19) | 6.3 | – | – | 15.2 ± 1.5 (13–17) | 10.3 |
| Anal body width | 9.7 | 10.6 ± 0.6 (9.7–12) | 5.8 | 10.1 ± 0.2 (10–10.6) | 2.3 | 10.2 ± 0.6 (9–11) | 6.5 |
| Tail/Vulva–Anus | 1.3 | 1.4 ± 0.2 (0.9–1.8) | 17.4 | – | – | 1 ± 0.1 (0.7–1.4) | 17.6 |
| Annulus width | 2.8 | 2.8 ± 0.1 (2.6–3) | 5.1 | 2.8 ± 0.2 (2.4–3) | 7.2 | 2.9 ± 0.2 (2.4–3.3) | 7.6 |
| Tail length | 115 | 114 ± 9.9 (94–128) | 8.7 | 115 ± 8.9 (101–130) | 7.7 | 76.8 ± 6.9 (65–87) | 9.0 |
| R | 216 | 222.4 ± 10 (210–240) | 4.5 | 214.6 ± 10.7 (201–233) | 5.0 | 188.5 ± 13.8 (170–210) | 7.3 |
| Rv | 85 | 86 ± 5.8 (76–96) | 6.7 | – | – | 65.6 ± 7.5 (58–83) | 11.5 |
| Ran | 54 | 53.2 ± 6.4 (41–59) | 12.2 | 53.8 ± 4 (48–60) | 7.5 | 31.7 ± 2.8 (28–36) | 9.0 |
| Rvan | 30 | 32.8 ± 4.5 (26–40) | 13.9 | – | – | 33.9 ± 6.3 (27–47) | 18.7 |
| Gubernaculum | – | – | – | 5.1 ± 0.3 (4.5–5.5) | 6.7 | – | – |
| Spicules | – | – | – | 14.3 ± 0.8 (13–15.5) | 6.3 | – | – |
Figure 1:
Figure 1: Coslenchus paramaritus n. sp. (line drawing). (A, B) Entire body in female and male. (C, D) Anterior portion. (E) Anterior end in dorso-ventral view. (I, K) Female tail. (J, L) Male tail. (F) Reproductive system. (G, H) Cross section at mid-body.
Figure 2:
Figure 2: Coslenchus paramaritus n. sp. (light photograph). (A) Female Anterior end (Lateral view). (B) Female Anterior end (Dorso-ventral view). (C) Male anterior end (Lateral view). (D) Male anterior end (Dorso-ventral view). (E) Entire body. (F) Cross section at mid-body. (G–I) Spicules and Bursa. (J) End portion of esophagus. (K, L) Female reproductive system. (M) Female tail. (N) Male tail. (A–D, F–L = 5 µm, E = 100 µm, M–N = 20 µm).
Figure 3:
Figure 3: Coslenchus cancellatus (Cobb, 1925) Siddiqi, 1978 (line drawing). (A) Entire body in female. (B) Anterior portion. (C, D) Anterior end in lateral view. (E) Anterior end in dorso-ventral view. (F) Cross section at mid-body. (G) Reproductive system. (H–M) Female tail.
Figure 4:
Figure 4: Coslenchus cancellatus (Cobb, 1925) Siddiqi, 1978, female (light photograph). (A) Entire body. (B, C) Anterior end in lateral view. (D) Anterior end in dorso-ventral view. (E) Posterior of esophagus. (F) Reproductive system. (G) Front view of vulva. (H) Front view of anus. (J) Lateral field. (K) tail. (L–O) Posterior of tail. (A = 20 µm, B–G, L–O = 5 µm, K = 50 µm.)
Figure 5:
Figure 5: The 50% majority rule consensus trees from Bayesian analysis generated from the D2–D3 expansion fragments of 28 S rRNA gene dataset under GTR+I+G model. Posterior probabilities for BI analysis more than 50% are given for appropriate clades. New sequences are indicated in bold.