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First report of Pratylenchus vulnus associated with apple in Tunisia Cover

First report of Pratylenchus vulnus associated with apple in Tunisia

Open Access
|Dec 2018

Figures & Tables

Figure 1

Photo micrographs of Pratylenchus vulnus specimens: females (B) and males (A), (C): lateral field at mid – body; (D): spermatheca; (E): pharyngeal region; (F) and (H): female tails; (G): vulval region; (I): male tail (Scale bars: A, B = 100 mm; C–I = 20 mm).
Photo micrographs of Pratylenchus vulnus specimens: females (B) and males (A), (C): lateral field at mid – body; (D): spermatheca; (E): pharyngeal region; (F) and (H): female tails; (G): vulval region; (I): male tail (Scale bars: A, B = 100 mm; C–I = 20 mm).

Figure 2

SEM micrograph of P. vulnus male morphology from Tunisia (A, B, C, D): anterior region, (E, F) : tail, (G) : lateral fields; sp : spicules, lig : 4 lateral lines ; anx : 3 labial annuli).
SEM micrograph of P. vulnus male morphology from Tunisia (A, B, C, D): anterior region, (E, F) : tail, (G) : lateral fields; sp : spicules, lig : 4 lateral lines ; anx : 3 labial annuli).

Figure 3

SEM micrograph of P. vulnus female morphology from Tunisia (A; B; C): anterior region, (D): lateral lines at vulva region, (E): vulva, (F, G): tail, (H): lateral fields).
SEM micrograph of P. vulnus female morphology from Tunisia (A; B; C): anterior region, (D): lateral lines at vulva region, (E): vulva, (F, G): tail, (H): lateral fields).

Figure 4

Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from partial 18S rRNA gene.
Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from partial 18S rRNA gene.

Figure 5

Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from D2-D3 expansion segments of the 28S rRNA.
Phylogenetic relationships between Pratylenchus vulnus identified in Tunisia and other Pratylenchus already deposited in GenBank. The Pratylenchus sequences aligned in MEGA with CLUSTALW and the phylogenetic tree made by MEGA version 7.0 based on the Neighbor-Joining method as inferred from D2-D3 expansion segments of the 28S rRNA.

Morphometrics of Pratylenchus vulnus from apples stoolbeds, nurseries (males and females), and orchards from four Tunisian regions (Kasserin, Kairouan, Monastir, and Zaghouan, Tunisia)_

Stoolbed
LocalityMM106 Chiha
CharactersFemalesMales
n55
L444.4 ± 29.36 (415.71–485.86)419.83 ± 32.43 (395.01–475.2)
Stylet length (µm)14.29± 0.16 (14.17–14.55)14.85 ± 0.21 (14.56–15.1)
Spicules (µm) 16.88± 0.26 (16.58–17.2)
Gubernaculum (µm) 4.8± 0.39 (4.2–5.25)
V or T81.22± 0.73 (80.55–82.39)34.02± 1.17 (32.11–35.2)
a26.73 ± 1.32 (24.59–28.1)28.99 ± 2.76 (25.2–31.4)
b7.62 ± 0.55 (6.87–8.16)6.72 ± 0.66 (5.88–7.5)
b′4.92 ± 0.9 (3.76–5.66)4.85 ± 0.27 (4.55–5.22)
c19.11 ± 0.44 (18.57–19.5)20.75 ± 0.55 (19.88–21.3)
c′2.29 ± 0.22 (2.01–2.58)2.14 ± 0.1 (2.01–2.28)
Nursery females
LocalityLorca ChihaMeski SMCCSPS
CharactersFemalesFemales
n55
L556.71 ± 33.21 (521.32–607.49)512.48 ± 51.46 (450.11–578.12)
Stylet length (µm)15. 45± 0.31 (15.17–15.93)14. 44± 0.6 (13.69–15.19)
Spicules (µm)
Gubernaculum (µm)
V or T78.77± 1.31 (76.95–80.55)79.14 ± 1.74 (76.58–81.3)
a28.8 ± 4.12 (25.93–35.73)28.88 ± 3.72 (25.72–35.33)
b7.82 ± 1.33 (6.1–9.56)7.97 ± 0.84 (7.12–9.18)
b′5.26 ± 0.11 (5.16–5.41)4.27 ± 0.27 (3.88–4.55)
c20.22 ± 1.91 (18.17–22.7)21.79 ± 2.81 (18.68–25.5)
c′2.44 ± 0.27 (2.08–2.78)2.33 ± 0.33 (2.04–2.91)
Nursery males
LocalityLorca ChihaAnna Manzel NourMeski SMCCSPS
CharactersMalesMalesMales
n555
L483.75 ± 10.58 (468.63–492.7)478.62 ± 30.09 (431.65–503.93)485.52 ± 34.38(451.12–540.76)
Stylet length (µm)14.81 ± 0.49 (14.41–15.66)14.46 ± 0.75 (13.49–15.26)14.31 ± 0.28 (14.01–14.66)
Spicules (µm)17.07± 1.35 (15.47–19.14)19.16± 0.61 (18.25–19.65)18.15 ± 0.58 (17.37–19)
Gubernaculum (µm)4.92± 0.34 (4.46–5.31)5.26± 0.27 (4.95–5.66)5.07 ± 0.43 (4.56–5.72)
V or T35.38± 2.75 (32–38.17)37.56± 2.55 (33.72–40.6)36.42 ± 2.76 (34.05–40.54)
a28.85 ± 1.45 (26.5–30.3)29.37 ± 2.33 (25.87–32.15)27.01 ± 1.37 (25.36–28.91)
b8.24 ± 1.21 (7.23–10.22)9.11 ± 0.38 (8.66–9.67)9.2 ± 1.01 (8.14–10.5)
b′5.31 ± 0.1 (5.2–5.43)4.29 ± 0.45 (3.77–4.95)4.85 ± 0.53 (4.47–5.75)
c19.89 ± 1.48 (17.86–21.62)20.95 ± 1.46 (18.7–22.47)20.16 ± 1.83 (18.85–23.37)
c′2.29 ± 0.18 (2.09–2.51)2.06 ± 0.08 (2–2.21)2.26 ± 0.21 (1.92–2.45)
Orchads
LocalityLorca ChihaAnna Manzel NourMeski SMCCSPS
CharactersMalesMalesMales
n555
L483.75 ± 10.58 (468.63–492.7)478.62 ± 30.09 (431.65–503.93)485.52 ± 34.38(451.12–540.76)
Stylet length (µm)14.81 ± 0.49 (14.41–15.66)14.46 ± 0.75 (13.49–15.26)14.31 ± 0.28 (14.01–14.66)
Spicules (µm)17.07± 1.35 (15.47–19.14)19.16± 0.61 (18.25–19.65)18.15 ± 0.58 (17.37–19)
Gubernaculum (µm)4.92± 0.34 (4.46–5.31)5.26± 0.27 (4.95–5.66)5.07 ± 0.43 (4.56–5.72)
V or T35.38± 2.75 (32–38.17)37.56± 2.55 (33.72–40.6)36.42 ± 2.76 (34.05–40.54)
a28.85 ± 1.45 (26.5–30.3)29.37 ± 2.33 (25.87–32.15)27.01 ± 1.37 (25.36–28.91)
b8.24 ± 1.21 (7.23–10.22)9.11 ± 0.38 (8.66–9.67)9.2 ± 1.01 (8.14–10.5)
b′5.31 ± 0.1 (5.2–5.43)4.29 ± 0.45 (3.77–4.95)4.85 ± 0.53 (4.47–5.75)
c19.89 ± 1.48 (17.86–21.62)20.95 ± 1.46 (18.7–22.47)20.16 ± 1.83 (18.85–23.37)
c′2.29 ± 0.18 (2.09–2.51)2.06 ± 0.08 (2–2.21)2.26 ± 0.21 (1.92–2.45)
DOI: https://doi.org/10.21307/jofnem-2018-056 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 579 - 586
Published on: Dec 3, 2018
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2018 Noura Chihani-Hammas, Lobna Hajji-Hedfi, Hajer Regaieg, Asma Larayedh, Ahmed Badiss, Yu Qing, Horrigue-Raouani Najet, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.