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Viral Metagenomics of the Bharal (Pseudois nayaur) within the Qinghai-Tibet Plateau Revealed Diverse Viruses Cover

Viral Metagenomics of the Bharal (Pseudois nayaur) within the Qinghai-Tibet Plateau Revealed Diverse Viruses

Open Access
|Jun 2025

References

  1. Black RE, Perin J, Yeung D, Rajeev T, Miller J, Elwood SE, Platts-Mills JA. Estimated global and regional causes of deaths from diarrhoea in children younger than 5 years during 2000-21: A systematic review and Bayesian multinomial analysis. Lancet Glob Health. 2024 Jun;12(6):e919–e928. https://doi.org/10.1016/s2214-109x(24)00078-0
  2. Conceição-Neto N, Zeller M, Lefrère H, De Bruyn P, Beller L, Deboutte W, Yinda CK, Lavigne R, Maes P, Van Ranst M, et al. Modular approach to customise sample preparation procedures for viral metagenomics: A reproducible protocol for virome analysis. Sci Rep. 2015 Nov;5:16532. https://doi.org/10.1038/srep16532
  3. Deng T, Wang X, Fortelius M, Li Q, Wang Y, Tseng ZJ, Takeuchi GT, Saylor JE, Säilä LK, Xie G. Out of Tibet: Pliocene woolly rhino suggests high-plateau origin of Ice Age megaherbivores. Science. 2011 Sep;333(6047):1285–1288. https://doi.org/10.1126/science.1206594
  4. Harris RB. Pseudois nayaur. The IUCN Red List of Threatened Species. 2014:e.T61513537A64313015.
  5. Hoyles L, McCartney AL, Neve H, Gibson GR, Sanderson JD, Heller KJ, van Sinderen D. Characterization of virus-like particles associated with the human faecal and caecal microbiota. Res Microbiol. 2014 Dec;165(10):803–812. https://doi.org/10.1016/j.resmic.2014.10.006
  6. Hu X, Wang X, He Y, Hu M, Jin L, Zhang H, Jin Y, Wang B, Yue J, Liang S, et al. Genetic characteristics of classical astroviruses in Shenzhen, China, 2016–2019. J Med Virol. 2023 Jul;95(7):e28902. https://doi.org/10.1002/jmv.28902
  7. Huson DH, Beier S, Flade I, Górska A, El-Hadidi M, Mitra S, Ruscheweyh HJ, Tappu R. MEGAN Community Edition – Interactive exploration and analysis of large-scale microbiome sequencing data. PLoS Comput Biol. 2016 Jun;12(6):e1004957. https://doi.org/10.1371/journal.pcbi.1004957
  8. Jian Y, Zhang X, Wang G, Wang G, Li X, Ma L, Liang Q, Li C, Zhang Y. First report on the molecular detection of Enterocytozoon bieneusi in livestock and wildlife around Qinghai Lake in the Qing-hai-Tibetan Plateau area, China. Int J Parasitol Parasites Wildl. 2023 Apr;21:110–115. https://doi.org/10.1016/j.ijppaw.2023.04.012
  9. Jiang Z, Migmar W. [Review on the study of blue sheep (Pseudois nayaur)] (in Chinese). Chin J Ecol. 2020;39(7):2426-2435.
  10. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, et al. Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012 Jun;28(12):1647–1649. https://doi.org/10.1093/bioinformat-ics/bts199
  11. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol. 2018 Jun;35(6):1547–1549. https://doi.org/10.1093/molbev/msy096
  12. Liu H, Zhao X, Xu S, Zhao L, Han X, Xu X, Zhao N, Hu L, Luo C, Wang X, et al. Multi-omics reveal the gut microbiota-mediated severe foraging environment adaption of small wild ruminants in the Three-River-Source National Park, China. Integr Zool. 2024 May:1–20. https://doi.org/10.1111/1749-4877.12830
  13. Liu Z, Yang S, Wang Y, Shen Q, Yang Y, Deng X, Zhang W, Delwart E. Identification of a novel human papillomavirus by metagenomic analysis of vaginal swab samples from pregnant women. Virol J. 2016 Jul;13:122. https://doi.org/10.1186/s12985-016-0583-6
  14. Liu Z, Zhu Z, Gao H, Zhao C, Sun Y, Teng L. Comparative analysis of winter diets and habitat use by the sympatric blue sheep (Pseudois nayaur) and Alashan red deer (Cervus alashanicus) in the Helan Mountains, China. Folia Zool. 2018;67(1):43–53. https://doi.org/10.25225/fozo.v67.i2.a6.2018
  15. Lu X, Gong G, Zhang Q, Yang S, Wu H, Zhao M, Wang X, Shen Q, Ji L, Liu Y, et al. Metagenomic analysis reveals high diversity of gut viromes in yaks (Bos grunniens) from the Qinghai-Tibet Plateau. Commun Biol. 2024 Sep;7(1):1097. https://doi.org/10.1038/s42003-024-06798-y
  16. Martin DP, Murrell B, Golden M, Khoosal A, Muhire B. RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol. 2015 May;1(1):vev003. https://doi.org/10.1093/ve/vev003
  17. Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, Lanfear R. IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Mol Biol Evol. 2020 May;37(5):1530–1534. https://doi.org/10.1093/mol-bev/msaa015
  18. Phan TG, Mori D, Deng X, Rajindrajith S, Ranawaka U, Fan Ng TF, Bucardo-Rivera F, Orlandi P, Ahmed K, Delwart E. Small circular single stranded DNA viral genomes in unexplained cases of human encephalitis, diarrhea, and in untreated sewage. Virology. 2015 Aug;482:98–104. https://doi.org/10.1016/j.virol.2015.03.011
  19. Prades Y, Pizarro R, Ruiz M, Moreno C, Avendaño LF, Luchsinger V. Cyclovirus detection in Chilean adults with and without community-acquired pneumonia. J Med Virol. 2021 Aug;93(8):4786–4793. https://doi.org/10.1002/jmv.27080
  20. Rivera R, Nollens HH, Venn-Watson S, Gulland FM, Wellehan JF Jr. Characterization of phylogenetically diverse astroviruses of marine mammals. J Gen Virol. 2010 Jan;91(Pt_1):166–173. https://doi.org/10.1099/vir.0.015222-0
  21. Sajewicz-Krukowska J, Domanska-Blicharz K. Nearly full-length genome sequence of a novel astrovirus isolated from chickens with ‘white chicks’ condition. Arch Virol. 2016 Sep;161(9):2581–2587. https://doi.org/10.1007/s00705-016-2940-6
  22. Smits SL, Zijlstra EE, van Hellemond JJ, Schapendonk CM, Bodewes R, Schürch AC, Haagmans BL, Osterhaus AD. Novel cyclovirus in human cerebrospinal fluid, Malawi, 2010–2011. Emerg Infect Dis. 2013;19(9):1511–1513. https://doi.org/10.3201/eid1909.130404
  23. Tse H, Chan WM, Tsoi HW, Fan RYY, Lau CCY, Lau SKP, Woo PCY, Yuen KY. Rediscovery and genomic characterization of bovine astroviruses. J Gen Virol. 2011 Aug;92(Pt_8):1888–1898. https://doi.org/10.1099/vir.0.030817-0
  24. Vu DL, Bosch A, Pintó RM, Guix S. Epidemiology of classic and novel human astrovirus: Gastroenteritis and beyond. Viruses. 2017 Feb;9(2):33. https://doi.org/10.3390/v9020033
  25. Wang X, Zhang Z, Li B, Hao W, Yin W, Ai S, Han J, Wang R, Duan Z. Depicting fecal microbiota characteristic in yak, cattle, yak-cattle hybrid and Tibetan sheep in different eco-regions of Qinghai-Tibetan Plateau. Microbiol Spectr. 2022 Aug;10(4):e0002122. https://doi.org/10.1128/spectrum.00021-22
  26. Wohlgemuth N, Honce R, Schultz-Cherry S. Astrovirus evolution and emergence. Infect Genet Evol. 2019 Apr;69:30–37. https://doi.org/10.1016/j.meegid.2019.01.009
  27. Wu S, Wang Y, Wang Z, Shrestha N, Liu J. Species divergence with gene flow and hybrid speciation on the Qinghai-Tibet Plateau. New Phytol. 2022 Apr;234(2):392–404. https://doi.org/10.1111/nph.17956
  28. Yang L, Wang Y, Zhang Z, He S. Comprehensive transcriptome analysis reveals accelerated genic evolution in a Tibet fish, Gymnodiptychus pachycheilus. Genome Biol Evol. 2014 Dec;7(1):251–261. https://doi.org/10.1093/gbe/evu279
  29. Zhang QX, Wang Y, Li Y, Han SY, Wang B, Yuan GH, Zhang PY, Yang ZW, Wang SL, Chen JY, et al. Vector-borne pathogens with veterinary and public health significance in Melophagus ovinus (sheep ked) from the Qinghai-Tibet Plateau. Pathogens. 2021 Feb;10(2):249. https://doi.org/10.3390/pathogens10020249
  30. Zhang W, Yang S, Shan T, Hou R, Liu Z, Li W, Guo L, Wang Y, Chen P, Wang X, et al. Virome comparisons in wild-diseased and healthy captive giant pandas. Microbiome. 2017 Aug;5(1):90. https://doi.org/10.1186/s40168-017-0308-0
DOI: https://doi.org/10.33073/pjm-2025-012 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 143 - 152
Submitted on: Nov 15, 2024
Accepted on: Apr 9, 2025
Published on: Jun 18, 2025
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 ERCONG ZHANG, GA GONG, SHIYIN HUANG, YINGYING BAO, LIKAI JI, SHIXING YANG, QUAN SHEN, XIAOCHUN WANG, PING WU, YUWEI LIU, WEN ZHANG, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.