Fig. 1
Fig. 2
The adsorption rate of phage P2 on cell wall after chemical and enzyme treatment_
| Treatment | Phage P2 adsorption (%) (mean ± S.D.) |
|---|---|
| none (control) | 97.26 ± 1.21a |
| 1% SDS (30 min, 37°C) | 98.26 ± 4.08a |
| 50 U/ml lysozyme (30 min, 37°C) | 75.78 ± 3.01b |
| 0.1 mg/ml proteinase K (30 min, 37°C) | 97.80 ± 1.81a |
| 5% TCA (15 min, 100°C) | 57.14 ± 6.25c |
Predicted function genes of L_ plantarum P2_
| CDS | Strand | Predicted function | Function |
|---|---|---|---|
| CDS12 | + | terminase small subunit | packaging |
| CDS14 | + | terminase large subunit | |
| CDS15 | + | portal protein | |
| CDS16 | + | prohead protease | structure |
| CDS17 | + | major capsid protein | |
| CDS18 | + | putative tail protein | |
| CDS20 | + | head-tail joining protein | |
| CDS21 | + | head-tail adaptor | |
| CDS22 | + | tail protein | |
| CDS23 | + | major tail protein | |
| CDS25 | + | tape measure protein | |
| CDS26 | + | distal tail protein | |
| CDS27 | + | baseplate protein tail-like protein | |
| CDS28 | + | tail fiber protein | |
| CDS57 | – | membrane protein | |
| CDS35 | + | integrase | host interaction |
| CDS59 | – | ATP/GTP- binding protein | regulation |
| CDS48 | – | PemK family transcriptional regulator | |
| CDS83 | + | putative DNA binding protein | |
| CDS37 | – | DNA polymerase | DNA replication |
| CDS58 | – | DNA polymerase | |
| CDS72 | + | DNA helicase | |
| CDS73 | + | DNA primase | |
| CDS74 | + | single-stranded-DNA-specific exonuclease | |
| CDS1 | – | HNH endonuclease | |
| CDS3 | – | HNH endonuclease | |
| CDS11 | + | HNH endonuclease | |
| CDS38 | – | HNH endonuclease | |
| CDS41 | – | HNH endonuclease | |
| CDS45 | – | HNH endonuclease | |
| CDS56 | – | HNH endonuclease | |
| CDS65 | – | HNH homing endonuclease | |
| tRNA | + | tRNA-Pro | |
| tRNA | + | tRNA-Gly | |
| CDS44 | – | extracellular transglycosylase | additional function |
| CDS69 | + | deoxynucleoside kinase | |
| CDS96 | thymidine kinase |