Table I
The adsorption rate of phage P2 on cell wall after chemical and enzyme treatment.
| Treatment | Phage P2 adsorption (%) (mean ± S.D.) |
|---|---|
| none (control) | 97.26 ± 1.21a |
| 1% SDS (30 min, 37°C) | 98.26 ± 4.08a |
| 50 U/ml lysozyme (30 min, 37°C) | 75.78 ± 3.01b |
| 0.1 mg/ml proteinase K (30 min, 37°C) | 97.80 ± 1.81a |
| 5% TCA (15 min, 100°C) | 57.14 ± 6.25c |
Fig. 1
Circular representation of the Lactiplantibacillus plantarum phage P2 genome. The innermost circle indicates the GC skew on the positive and negative strand (green and purple). The second circle indicates the GC content (black). The outer circle indicates predicted CDS located on the positive and negative DNA strand (lavender). Red indicates tRNA coding genes.
Table II
Predicted function genes of L. plantarum P2.
| CDS | Strand | Predicted function | Function |
|---|---|---|---|
| CDS12 | + | terminase small subunit | packaging |
| CDS14 | + | terminase large subunit | |
| CDS15 | + | portal protein | |
| CDS16 | + | prohead protease | structure |
| CDS17 | + | major capsid protein | |
| CDS18 | + | putative tail protein | |
| CDS20 | + | head-tail joining protein | |
| CDS21 | + | head-tail adaptor | |
| CDS22 | + | tail protein | |
| CDS23 | + | major tail protein | |
| CDS25 | + | tape measure protein | |
| CDS26 | + | distal tail protein | |
| CDS27 | + | baseplate protein tail-like protein | |
| CDS28 | + | tail fiber protein | |
| CDS57 | – | membrane protein | |
| CDS35 | + | integrase | host interaction |
| CDS59 | – | ATP/GTP- binding protein | regulation |
| CDS48 | – | PemK family transcriptional regulator | |
| CDS83 | + | putative DNA binding protein | |
| CDS37 | – | DNA polymerase | DNA replication |
| CDS58 | – | DNA polymerase | |
| CDS72 | + | DNA helicase | |
| CDS73 | + | DNA primase | |
| CDS74 | + | single-stranded-DNA-specific exonuclease | |
| CDS1 | – | HNH endonuclease | |
| CDS3 | – | HNH endonuclease | |
| CDS11 | + | HNH endonuclease | |
| CDS38 | – | HNH endonuclease | |
| CDS41 | – | HNH endonuclease | |
| CDS45 | – | HNH endonuclease | |
| CDS56 | – | HNH endonuclease | |
| CDS65 | – | HNH homing endonuclease | |
| tRNA | + | tRNA-Pro | |
| tRNA | + | tRNA-Gly | |
| CDS44 | – | extracellular transglycosylase | additional function |
| CDS69 | + | deoxynucleoside kinase | |
| CDS96 | thymidine kinase |
Fig. 2
Comparative phylogenetic analysis. Comparative phylogenetic analysis of nucleotide sequences was aligned by ClustalW and performed using the neighbor-joining method in MEGA5.2. Numbers associated with each branch represent bootstrap values.