Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2

Hanfang Zhu; She Guo; Jie Zhao; Hafiz Arbab Sakandar; Ruirui Lv; Qiannan Wen; Xia Chen

doi:10.33073/pjm-2022-037

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Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2

Polish Journal of Microbiology

Volume 71 (2022): Issue 3 (September 2022)

By: Hanfang Zhu, She Guo, Jie Zhao, Hafiz Arbab Sakandar, Ruirui Lv, Qiannan Wen and Xia Chen

Open Access

|Sep 2022

Abstract

Phage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that this phage belonged to the Siphoviridae family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.

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Articles in this issue

DOI: https://doi.org/10.33073/pjm-2022-037 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331

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Language: English

Page range: 421 - 428

Submitted on: May 25, 2022

Accepted on: Jul 22, 2022

Published on: Sep 24, 2022

Published by: Polish Society of Microbiologists

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Lactiplantibacillus plantarum phage P2,

genome characteristics,

Related subjects:

Microbiology and virology

© 2022 Hanfang Zhu, She Guo, Jie Zhao, Hafiz Arbab Sakandar, Ruirui Lv, Qiannan Wen, Xia Chen, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 71 (2022): Issue 3 (September 2022)