Table I
Sequences of the forward and reverse primers of four genes used in Real-time PCR.
| Target gene | Primer sequence (5′ to 3′) | Size (bp) |
|---|---|---|
| GAPDH | Forward: GGGGTCCCAGCTTAGGTTCA Reverse: ACGGCCAAATCCGTTCACA | 95 |
| mip | Forward: AAGAAAACCTCTCCCTAGCC Reverse: CTGAAGGTTTCCCTGATATTG | 139 |
| ompA | Forward: GCGGCATTCAACCTCGTT Reverse: CCTTGAGTGATGCCTACATTGG | 85 |
| pmp18D | Forward: TCCACTGGGATGATCACCAATA Reverse: GCATAGAAAGCGTATCGAGAATCAC | 81 |
Fig. 1
mRNA expression levels of the ompA, pmp18D, and mip genes as fold change in pregnant and nonpregnant mice tissues at different pregnancy times by RT-qPCR. Data are presented as means of values for six mice (columns) ± SEM (error bars).
Table II
Wilcoxon signed-rank test analysis of the mip, pmp18D, and ompA genes expression between the pregnant groups in comparison to the nonpregnant groups at each time interval of pregnancy.
| Group | Gene type | Period of pregnancy (days) | p-values |
|---|---|---|---|
| Pregnant group – nonpregnant | mip | 10 15 20 | 0.593 0.655 0.655 |
| Pregnant group – nonpregnant | pmp18D | 10 15 20 | 0.109 0.593 0.593 |
| Pregnant group – nonpregnant | ompA | 10 15 20 | 0.285 1.000 0.655 |