Fig. 1.
Cultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 180.
Fig. 2.
Morphology of initial cultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 180 (stained by Ziehl-Neelsen). Bar = 10 µm.
Fig. 3.
Subcultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 115 (at 37°C), c) passage No. 115 (at 3°C), d) passage No. 135, e) passage No. 143, f) passage No. 171 (at 37°C), g) passage No. 171 (at 3°C), h) passage No. 180.
Fig. 4.
Morphology of Mycobacterium bovis subcultures: a) passage No. 54, b) passage No. 115 (at 37°C), c) passage No. 115 (at 3°C), d) passage No. 135, e) passage No. 143, f) passage No. 171 (at 37°C), g) passage No. 171 (at 3°C), h) passage No. 180; 1 – rod-shaped bacteria, 2 – thread-like forms, 3 – grains (elementary bodies), 4 – output of grains from thread-like forms, 5 – L-forms. Bar = 10 µm.
Fig. 5.
The spleen of the guinea pig: 1) normal, 2) with tuberculous foci.
Fig. 6.
Growth of pathogenic Mycobacterium bovis strain and its subcultures (variants) on nutrient media. FPA – nutrient agar, FPB – nutrient broth.
Fig. 7.
Catalase activity of Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction.
Fig. 8.
Dehydrogenase activity of Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction after 24 hours.
Fig. 9.
Reduction of nitrates by Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction.
Fig. 10.
Tween-80 hydrolysis by Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs: A) after five days, B) after ten days.
Table I
The results of DNA amplification of mycobacteria
| Type of mycobacteria | Strain of mycobacteria | Result of detection of amplification products |
|---|---|---|
| Mycobacterium bovis | fast-growing (original) | + |
| after the long-term storage and reinoculation of cultures on the nutrient medium), passages 54, 115, 135, 135, 171, and 180 | – | |
| Vallee strain | + | |
| BCG strain | + |