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The Effect of Long-Term Storage on Mycobacterium bovis Cover

The Effect of Long-Term Storage on Mycobacterium bovis

Polish Journal of Microbiology
Volume 70 (2021): Issue 3 (September 2021)
By: OLEXIY TKACHENKO,  NATALI KOZAK,  MARYNA BILAN,  VOLODYMYR HLEBENIUK,  NATALIA ALEKSEEVA,  LILIYA KOVALEVA,  VITALII NEDOSEKOV and  OLEXANDR GALATIUK  
Open Access
|Sep 2021

Figures & Tables

Fig. 1.

Cultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 180.
Cultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 180.

Fig. 2.

Morphology of initial cultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 180 (stained by Ziehl-Neelsen). Bar = 10 µm.
Morphology of initial cultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 180 (stained by Ziehl-Neelsen). Bar = 10 µm.

Fig. 3.

Subcultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 115 (at 37°C), c) passage No. 115 (at 3°C), d) passage No. 135, e) passage No. 143, f) passage No. 171 (at 37°C), g) passage No. 171 (at 3°C), h) passage No. 180.
Subcultures of Mycobacterium bovis: a) passage No. 54, b) passage No. 115 (at 37°C), c) passage No. 115 (at 3°C), d) passage No. 135, e) passage No. 143, f) passage No. 171 (at 37°C), g) passage No. 171 (at 3°C), h) passage No. 180.

Fig. 4.

Morphology of Mycobacterium bovis subcultures: a) passage No. 54, b) passage No. 115 (at 37°C), c) passage No. 115 (at 3°C), d) passage No. 135, e) passage No. 143, f) passage No. 171 (at 37°C), g) passage No. 171 (at 3°C), h) passage No. 180; 1 – rod-shaped bacteria, 2 – thread-like forms, 3 – grains (elementary bodies), 4 – output of grains from thread-like forms, 5 – L-forms. Bar = 10 µm.
Morphology of Mycobacterium bovis subcultures: a) passage No. 54, b) passage No. 115 (at 37°C), c) passage No. 115 (at 3°C), d) passage No. 135, e) passage No. 143, f) passage No. 171 (at 37°C), g) passage No. 171 (at 3°C), h) passage No. 180; 1 – rod-shaped bacteria, 2 – thread-like forms, 3 – grains (elementary bodies), 4 – output of grains from thread-like forms, 5 – L-forms. Bar = 10 µm.

Fig. 5.

The spleen of the guinea pig: 1) normal, 2) with tuberculous foci.
The spleen of the guinea pig: 1) normal, 2) with tuberculous foci.

Fig. 6.

Growth of pathogenic Mycobacterium bovis strain and its subcultures (variants) on nutrient media. FPA – nutrient agar, FPB – nutrient broth.
Growth of pathogenic Mycobacterium bovis strain and its subcultures (variants) on nutrient media. FPA – nutrient agar, FPB – nutrient broth.

Fig. 7.

Catalase activity of Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction.
Catalase activity of Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction.

Fig. 8.

Dehydrogenase activity of Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction after 24 hours.
Dehydrogenase activity of Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction after 24 hours.

Fig. 9.

Reduction of nitrates by Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction.
Reduction of nitrates by Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs (repeatedness, %): “–” – negative reaction, “±” – doubtful reaction, “+”, “++”, “+++” – the magnitude of the positive reaction.

Fig. 10.

Tween-80 hydrolysis by Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs: A) after five days, B) after ten days.
Tween-80 hydrolysis by Mycobacterium bovis initial subcultures and the subcultures passaged through the guinea pigs: A) after five days, B) after ten days.

The results of DNA amplification of mycobacteria

Type of mycobacteriaStrain of mycobacteriaResult of detection of amplification products
Mycobacterium bovisfast-growing (original)+
after the long-term storage and reinoculation of cultures on the nutrient medium), passages 54, 115, 135, 135, 171, and 180–
Vallee strain+
BCG strain+
DOI: https://doi.org/10.33073/pjm-2021-031 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 327 - 337
Submitted on: Mar 11, 2021
|
Accepted on: Jul 14, 2021
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Published on: Sep 17, 2021
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
tuberculosis,
survival,
morphology,
acid-fast,
variability
Related subjects:
Life sciences,
Microbiology and virology

© 2021 OLEXIY TKACHENKO, NATALI KOZAK, MARYNA BILAN, VOLODYMYR HLEBENIUK, NATALIA ALEKSEEVA, LILIYA KOVALEVA, VITALII NEDOSEKOV, OLEXANDR GALATIUK, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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