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Antibacterial Activity and Mode of Action of β-caryophyllene on Bacillus cereus Cover

Antibacterial Activity and Mode of Action of β-caryophyllene on Bacillus cereus

Polish Journal of Microbiology
Volume 69 (2020): Issue 1 (March 2020)
By: CHEW-LI MOO,  SHUN-KAI YANG,  MOHD-AZURAIDI OSMAN,  MOHD HAFIS YUSWAN,  JIUN-YAN LOH,  WEI-MENG LIM,  SWEE-HUA-ERIN LIM and  KOK-SONG LAI  
Open Access
|Mar 2020

Figures & Tables

Fig. 1.

Time-kill analysis of B. cereus treated with BCP. At MIC 2.5% BCP (v/v), the bacteria were killed within 2 hours. Cells treated at half MIC showed a suppressed and slower growth rate than the untreated cells.
Time-kill analysis of B. cereus treated with BCP. At MIC 2.5% BCP (v/v), the bacteria were killed within 2 hours. Cells treated at half MIC showed a suppressed and slower growth rate than the untreated cells.

Fig. 2.

Assays performed to evaluate the effects of BCP on the membrane surface charge and integrity of B. cereus. (A) Zeta-potential value (mV) of untreated (–5.460 mV) and BCP-treated cells (–3.980 mV); (B) Nucleic acid content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment; and (C) Protein content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment. Results were presented in mean ± SD for triplicates and considered as significant when * p < 0.050.
Assays performed to evaluate the effects of BCP on the membrane surface charge and integrity of B. cereus. (A) Zeta-potential value (mV) of untreated (–5.460 mV) and BCP-treated cells (–3.980 mV); (B) Nucleic acid content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment; and (C) Protein content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment. Results were presented in mean ± SD for triplicates and considered as significant when * p < 0.050.

Fig. 3.

The influx and efflux of EtBr in B. cereus (A) EtBr influx assay with untreated and BCP-treated cells exposed to 1 mg/l of EtBr for 60 minutes and (B) EtBr efflux assay with the cells treated with glucose or BCP only and in a combination of glucose and BCP.
The influx and efflux of EtBr in B. cereus (A) EtBr influx assay with untreated and BCP-treated cells exposed to 1 mg/l of EtBr for 60 minutes and (B) EtBr efflux assay with the cells treated with glucose or BCP only and in a combination of glucose and BCP.
DOI: https://doi.org/10.33073/pjm-2020-007 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 49 - 54
Submitted on: Oct 19, 2019
Accepted on: Jan 18, 2020
Published on: Mar 12, 2020
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
antibacterial,
β-caryophyllene,
intracellular leakage,
membrane damage,
zeta potential
Related subjects:
Life sciences,
Microbiology and virology

© 2020 CHEW-LI MOO, SHUN-KAI YANG, MOHD-AZURAIDI OSMAN, MOHD HAFIS YUSWAN, JIUN-YAN LOH, WEI-MENG LIM, SWEE-HUA-ERIN LIM, KOK-SONG LAI, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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