Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Lag phase duration and final OD after 96 hours of filamentous fungal and yeast growth in BioscreenC without or with AgNPs_
| Filamentous fungi and yeast species | Lag phase [h] | OD | ||
|---|---|---|---|---|
| at 96 h | compared to | |||
| SM [%] | initial [× folds] | |||
| SM: spores mix (control) | 10 | 1.592 | 100 | 36 |
| SM: spores mix +AgNPs | 20 | 1.308 | 82 | 5.8 |
| A. brasiliensis +AgNPs | 40 | 1.600 | 100 | 8.1 |
| P. pinophilum +AgNPs | 30 | 1.517 | 95 | 9.2 |
| P. variotii +AgNPs | ~ 68 | 0.633 | 40 | 3.7 |
| T. virens +AgNPs | 18 | 1.306 | 82 | 6.5 |
| C. globosum +AgNPs | 48 | 1.063 | 67 | 5.2 |
| C. albicans +AgNPs | 15 | 0.953 | – | 3.8 |
| Y. lipolytica +AgNPs | > 96 | 0.194 | – | 0 |
AgNP inhibition of the filamentous fungi growth_
| Fungal species | AgNPs in the medium [mg/l] | |||
|---|---|---|---|---|
| 0 | 2.14 | 4.28 | 21.4 | |
| Growth [%] | ||||
| Aspergillus brasiliensis | 100 | 100 | 83.1 | 3.74 |
| Trichoderma virens | 100 | 100 | 33.9 | 9.72 |
| Paecilomyces variotii | 100 | 100 | 0 | 0 |
| Penicillium pinophilum | 100 | 57.3 | 44.8 | 0 |
| Chaetomium globosum | 100 | 63.9 | 61.2 | 0 |
The size and distribution (inside, outside, and within the cell wall) of AgNPs after their interaction with microorganisms_
| AgNPs Size [nm] | [%] | Cell distribution according o the size [%] | |||
|---|---|---|---|---|---|
| Inside | Outside | In the cell wall | Total | ||
| 21.45 ± 5.9 | 79.1 | 37.4 | 51.6 | 11 | 100 |
| 54.49 ± 9.1 | 13.9 | 93.8 | 6.25 | 0 | 100 |
| 94.62 ± 18.16 | 7.0 | 85.7 | 14.3 | 0 | 100 |
| Total | 100 | 48.7 | 42.6 | 8.7 | 100 |