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Chemical Composition of Aspergillus creber Extract and Evaluation of its Antimicrobial and Antioxidant Activities Cover

Chemical Composition of Aspergillus creber Extract and Evaluation of its Antimicrobial and Antioxidant Activities

Polish Journal of Microbiology
Volume 68 (2019): Issue 3 (September 2019)
By: ,  ,  ,   and    
Open Access
|Sep 2019
Figures & tables

Figures & Tables

Fig. 1.

Total Ion Current chromatogram of Aspergillus creber extract obtained with UHPLC-MS/MS. The retention times of the peaks (1–10) and their corresponding molecules names are presented in Table I.

Table I

Metabolites of Aspergillus creber as determined by UHPLC-MS/MS. The metabolites were tentatively identified using molecular mass data, MS/MS analysis and references.

Peak No.Putative compound nameAdductionMeasured mass (m/z)Productions (m/z)tRa (min)Refb
1Asperlactone[M+H] +185.08081141.05444, 113.059481.73Vishwanath et al. 2009
2NI[M+Na] +211.06121195.03455, 133.028161.78-
3NI[M+H] +308.10992280.95587, 145.101043.38-
4NI[M+H] +327.04734309.18594, 191.153964.28-
5Emodin[M+H]-269.04590ND5.42Sulyok et al. 2007; Lehner et al. 2011; Micheluz et al. 2016
6NI[M+H]+251.09145233.09526, 204.093195.49-
7Sterigmatocystin[M+H]+325.07053ND6.62Lehner et al. 2011; Micheluz et al. 2016
8NI[M+Na] +423.25041405.27911, 239.148217.27-
9Deoxybrevianamide E[M+H]+352.20352ND8.04Lehner et al. 2011; Micheluz et al. 2016
10Norsolorinic acid[M+H]-369.09892ND8.24Micheluz et al. 2016

1 a - Retention time; b - Reference; ND - not detected; NI - not identified

Table 2.

Antimicrobial activity and minimum inhibitory concentration (MIC) of ethyl acetate extract of Aspergillus creber against human pathogens.

Zone of inhibition (mm)aMIC of ethyl acetate extract (mg/ml)
Ethyl acetate extractPositive controlb
Bacteria
Escherichia coli023.4 ± 0.3-
Klebsiella pneumoniae10.0 ± 0.325.0 ± 0.42.5
Pseudomonas aeruginosa020.8 ± 0.4-
Salmonella Typhi8.5 ± 0.621.5 ± 0.35
SABLc12.8 ± 0.319.8 ± 0.20.625
MRSEd14.0 ± 0.219.2 ± 0.20.625
Yeasts
Candida albicans20.6 ± 0.822.8 ± 0.40.325
Candida glabrata13.0 ± 0.325.7 ± 0.51.25

1a - Mean of three replicates (±) SD

2b - Chloramphenicol and ketoconazole were used as the positive control for bacteria and yeasts respectively

3c - Staphylococcus aureus producing beta lactamase

4d - Methicillin resistant Staphylococcus epidermidis

5 0- no zone of inhibition

Fig. 2.

Free radical-scavenging activities of Aspergillus creber extract and ascorbic acid (400 μg/ml) measured (a), in DPPH assay and (b), in ABTS assay.

Table III

Antioxidant activities of Aspergillus creber ethyl acetate extract by DPPH and ABTS assays and its total phenolic content.

ActivityExtractAscorbic acida
DPPHa89.28 ± 0.3291.39 ± 0.39
ABTSa92.93 ± 0.3093.03 ± 0.45
Total phenolic content (mg GAE/g)85.76 ± 0.96-

1a - Percentage of inhibition at a concentration of400 μg/ml. Values are mean of three replicates (±) SD

DOI: https://doi.org/10.33073/pjm-2019-033 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 309 - 316
Submitted on: Mar 18, 2019
Accepted on: May 7, 2019
Published on: Sep 3, 2019
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Antimicrobial activity,
antioxidant activity,
Aspergillus creber,
UHPLC-MS/MS,
Versicolores
Related subjects:
Life sciences,
Microbiology and virology

© 2019 AFAF SAKHRI, NOREDDINE KACEM CHAOUCHE, MARIA ROSARIA CATANIA, ALBERTO RITIENI, ANTONELLO SANTINI, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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