Metabolomic profiling and structural characterization of Ulva onhoi by HPTLC and NMR spectroscopy
References
- Abou-ElWafa, G. S. E., Shaaban, M., Shaaban, K. A., El-Naggar, M. E. E., & Laatsch, H. (2009). Three new unsaturated fatty acids from the marine green alga Ulva fasciata Delile. Zeitschrift für Naturforschung B, 64(10), 1199–1207.
https://doi.org/10.1515/znb-2009-1014 - Alamsjah, M. A., Hirao, S., Ishibashi, F., & Fujita, Y. (2005). Isolation and structure determination of algicidal compounds from Ulva fasciata. Bioscience, Biotechnology, and Biochemistry, 69(11), 2186–2192.
https://doi.org/10.1271/bbb.69.2186 - Alsufyani, T., Weiss, A., & Wichard, T. (2017). Time course exometabolomic profiling in the green marine macroalga Ulva (Chlorophyta) for identification of growth phase-dependent biomarkers. Marine Drugs, 15(1), 14.
https://doi.org/10.3390/md15010014 - Anggadiredja, J. T. (2009). Ethnobotany study of seaweed diversity and its utilization in Warambadi, Panguhalodo areas of East Sumba District. Jurnal Teknologi Lingkungan, 10(3), 297–310.
https://doi.org/10.29122/jtl.v10i3.1476 - Anjali, K. P., Sangeetha, B. M., Devi, G., Raghunathan, R., & Dutta, S. (2019). Bioprospecting of seaweeds (Ulva lactuca and Stoechospermum marginatum): The compound characterization and functional applications in medicine – A comparative study. Journal of Photochemistry and Photobiology B: Biology, 200, Article 111622.
https://doi.org/10.1016/j.jphotobiol.2019.111622 - Babich, O., Sukhikh, S., Larina, V., Kalashnikova, O., Kashirskikh, E., Prosekov, A., Noskova, S., Ivanova, S., Fendri, I., Smaoui, S., Abdelkafi, S., Michaud, P., & Dolganyuk, V. (2022). Algae: Study of edible and biologically active fractions, their properties and applications. Plants (Basel, Switzerland), 11(6), Article 780.
https://doi.org/10.3390/plants11060780 - Camarena-Gómez, M. T., Lähteenmäki-Uutela, A., & Spilling, K. (2022). Macroalgae production in Northern Europe: Business and government perspectives on how to regulate a novel blue bioeconomy. Aquaculture, 560(2), Article 738434.
https://doi.org/10.1016/j.aquaculture.2022.738434 - Cao, S., Yang, Y., Liu, S., Shao, Z., Chu, X., & Mao, W. (2022). Immunomodulatory activity in vitro and in vivo of a sulfated polysaccharide with novel structure from the green alga Ulva conglobata Kjellman. Marine Drugs, 20(7), Article 447.
https://doi.org/10.3390/md20070447 - Chakraborty, K., & Paulraj, R. (2010). Sesquiterpenoids with free-radical-scavenging properties from marine macroalga Ulva fasciata Delile. Food Chemistry, 122(1), 31–41.
https://doi.org/10.1016/j.foodchem.2010.02.012 - Chengkui, Z., Tseng, C. K., Junfu, Z., & Chang, C. F. (1984). Chinese seaweeds in herbal medicine. Hydrobiologia, 116/117, 152–154.
https://doi.org/10.1007/BF00027655 - Chi, Y., Zhang, M., Wang, X., Fu, X., Guan, H., & Wang, P. (2020). Ulvan lyase assisted structural characterization of Ulvan from Ulva pertusa and its antiviral activity against vesicular stomatitis virus. International Journal of Biological Macromolecules, 157, 75–82.
https://doi.org/10.1016/j.ijbiomac.2020.04.187 - Costa, S. P., Cotas, J., & Pereira, L. (2024). Laminar Ulva species: A multi-tool for humankind? Applied Sciences, 14(8), 3448.
https://doi.org/10.3390/app14083448 - Del Mondo, A., Sansone, C., & Brunet, C. (2022). Insights into the biosynthesis pathway of phenolic compounds in microalgae. Computational and Structural Biotechnology Journal, 20, 1901–1913.
https://doi.org/10.1016/j.csbj.2022.04.019 - Dumilag, R. V., & Javier, R. F. (2022). Ethnobotany of medicinal seaweeds of Ilocos Norte, Philippines. The Philippine Journal of Science, 151(3), 1135–1156.
https://doi.org/10.56899/151.03.28 - El Ashry, E. S. H., Atta-ur-Rahman, Choudhary, M. I., Kandil, S. H., El Nemr, A., Gulzar, T., & Shobier, A. H. (2011). Studies on the constituents of the green alga Ulva lactuca. Chemistry of Natural Compounds, 47(3), 335–338.
https://doi.org/10.1007/s10600-011-9926-0 - El-Bilawy, E. H., Al-Mansori, A. N. A., Alotibi, F. O., Al-Askar, A. A., Arishi, A. A., Teiba, I. I, Sabry, A. E.-N., Elsharkawy, M. M., Heflish, A. A., Behiry, S. I, & Abdelkhalek, A. (2022). Antiviral and antifungal of Ulva fasciata extract: HPLC analysis of polyphenolic compounds. Sustainability, 14(19), Article 12799.
https://doi.org/10.3390/su141912799 - El-Mesallamy, A. M. D., Mohamed, S. Z., Ali, Y. M., & Amer, T. N. (2021). Phytochemical constituents of Ulva lactuca and supplementation to improve the Nile tilapia (Oreochromis niloticus) haemato-biochemical status. Egyptian Journal of Chemistry, 64(5), 2663–2670.
https://doi.org/10.21608/ejchem.2021.60453.3296 - Flodin, C., & Whitfield, F. B. (1999). 4-Hydroxybenzoic acid: A likely precursor of 2,4,6-tribromophenol in Ulva lactuca. Phytochemistry, 51(2), 249–255.
https://doi.org/10.1016/S0031-9422(98)00754-7 - Ghaderiardakani, F., Langhans, L., Kurbel, V. B., Fenizia, S., & Wichard, T. (2022). Metabolite profiling reveals insights into the species-dependent cold stress response of the green seaweed holobiont Ulva (Chlorophyta). Environmental and Experimental Botany, 200, Article 104913.
https://doi.org/10.1016/j.envexpbot.2022.104913 - Ghallab, D. S., Shawky, E., Ibrahim, R. S., & Mohyeldin, M. M. (2022). Comprehensive metabolomics unveil the discriminatory metabolites of some Mediterranean Sea marine algae in relation to their cytotoxic activities. Scientific Reports, 12(1), 8094.
https://doi.org/10.1038/s41598-022-12265-7 - Gupta, V., & Kushwaha, H. R. (2017). Metabolic regulatory oscillations in intertidal green seaweed Ulva lactuca against tidal cycles. Scientific Reports, 7(1), 16430.
https://doi.org/10.1038/s41598-017-15994-2 - He, L., Jiang, B., Peng, Y., Zhang, X., & Liu, M. (2025). NMR based methods for metabolites analysis. Analytical Chemistry, 97(10), 5393–5406.
https://doi.org/10.1021/acs.analchem.4c06477 - He, Y., Hu, C., Wang, Y., Cui, D., Sun, X., Li, Y., & Xu, N. (2018). The metabolic survival strategy of marine macroalga Ulva prolifera under temperature stress. Journal of Applied Phycology, 30(6), 3611–3621.
https://doi.org/10.1007/s10811-018-1493-3 - Kammoun, I., Ben Salah, H., Ben Saad, H., Cherif, B., Droguet, M., Magné, C., Kallel, C., Boudawara, O., Hakim, A., Gharsallah, N., & Ben Amara, I. (2018). Hypolipidemic and cardioprotective effects of Ulva lactuca ethanolic extract in hypercholesterolemic mice. Archives of Physiology and Biochemistry, 124(4), 313–325.
https://doi.org/10.1080/13813455.2017.1401641 - Kendel, M., Wielgosz-Collin, G., Bertrand, S., Roussakis, C., Bourgougnon, N., & Bedoux, G. (2015). Lipid composition, fatty acids and sterols in the seaweeds Ulva armoricana and Solieria chordalis from Brittany (France): An analysis from nutritional, chemotaxonomic, and antiproliferative activity perspectives. Marine Drugs, 13(9), 5606–5628.
https://doi.org/10.3390/md13095606 - Kidgell, J. T., Magnusson, M., de Nys, R., & Glasson, C. R. K. (2019). Ulvan: A systematic review of extraction, composition and function. Algal Research, 39(2), Article 101422.
https://doi.org/10.1016/j.algal.2019.101422 - Lahaye, M., & Robic, A. (2007). Structure and functional properties of Ulvan, a polysaccharide from green seaweeds. Biomacromolecules, 8(6), 1765–1774.
https://doi.org/10.1021/bm061185q - Laramore, S. E., Wills, P. S., & Hanisak, M. D. (2022). Seasonal variation in the nutritional profile of Ulva lactuca produced in a land-based IMTA system. Aquaculture International, 30(6), 3067–3079.
https://doi.org/10.1007/s10499-022-00950-3 - Li, Z., He, Y., He, H., Zhou, W., Li, M., Lu, A., Che, T., & Shen, S. (2023). Purification identification and function analysis of ACE inhibitory peptide from Ulva prolifera protein. Food Chemistry, 401, Article 134127.
https://doi.org/10.1016/j.foodchem.2022.134127 - Mannino, A. M., & Micheli, C. (2020). Ecological function of phenolic compounds from Mediterranean fucoid algae and seagrasses: An overview on the genus Cystoseira sensu lato and Posidonia oceanica (L.) Delile. Journal of Marine Science and Engineering, 8(1), Article 19.
https://doi.org/10.3390/jmse8010019 - Marques, A., Ferreira, J., Abreu, H., Pereira, R., Pinto, D., Silva, A., Gaivão, I., & Pacheco, M. (2021). Comparative genoprotection ability of wild-harvested vs. aqua-cultured Ulva rigida coupled with phytochemical profiling. European Journal of Phycology, 56(1), 105–118.
https://doi.org/10.1080/09670262.2020.1778796 - Martin, M. M. (2017). Technical aspects and pitfalls of LC/MS hyphenation. In S. Kromidas (Ed.), The HPLC-MS handbook for practitioners (pp. 19–71).
https://doi.org/10.1002/9783527809202.ch2 - Mohy El-Din, S. M., & Alagawany, N. I. (2019). Phytochemical constituents and anticoagulation property of marine algae Gelidium crinale, Sargassum hornschuchii and Ulva linza. Thalassas, 35(5), 381–397.
https://doi.org/10.1007/s41208-019-00142-6 - Nwokorogu, V. C., & Sabiu, S. (2026). Targeted and untargeted metabolomics for algal characterization and application. Algae and algal metabolites: Emerging applications, trends and prospects (pp. 1–32). Springer Nature Switzerland.
https://doi.org/10.1007/978-3-031-80866-1_34-2 - Pakingking, R. Jr., Usero, R., de Jesus-Ayson, E. G. T., Logronio, D. J., & Caipang, C. M. (2022). Phytochemical composition, antioxidant, and antibacterial activity of the Philippine marine green alga (Ulva pertusa). International Aquatic Research, 14(1), 51–62.
https://doi.org/10.22034/iar.2022.1946410.1217 - Pappou, S., Dardavila, M. M., Savvidou, M. G., Louli, V., Magoulas, K., & Voutsas, E. (2022). Extraction of bioactive compounds from Ulva lactuca. Applied Sciences, 12(4), Article 2117.
https://doi.org/10.3390/app12042117 - Pereira, L. (2015). Seaweed flora of the European North Atlantic and Mediterranean. In S.-K. Kim (Ed.), Springer handbook of marine biotechnology (pp. 65–178). Springer Berlin Heidelberg.
https://doi.org/10.1007/978-3-642-53971-8_6 - Pirian, K., Piri, K., Sohrabipour, J., Tamadoni Jahromi, S., & Blomster, J. (2016). Nutritional and phytochemical evaluation of the common green algae, Ulva spp. (Ulvophyceae), from the Persian Gulf. Fundamental and Applied Limnology, 188(4), 285–297.
https://doi.org/10.1127/fal/2016/0947 - Putra, N. R., Fajriah, S., Qomariyah, L., Dewi, A. S., Rizkiyah, D. N., Irianto, I., Rusmin, D., Melati, M., Trisnawati, N. W., Darwati, I., & Arya, N. N. (2024). Exploring the potential of Ulva lactuca: Emerging extraction methods, bioactive compounds, and health applications—A perspective review. South African Journal of Chemical Engineering, 47(1), 233–245.
https://doi.org/10.1016/j.sajce.2023.11.017 - Roach, L. A., Meyer, B. J., Fitton, J. H., & Winberg, P. (2022). Improved plasma lipids, anti-inflammatory activity, and microbiome shifts in overweight participants: Two clinical studies on oral supplementation with algal sulfated polysaccharide. Marine Drugs, 20(8), Article 500.
https://doi.org/10.3390/md20080500 - Ruan, Q., Chen, Y., Wen, J., Qiu, Y., Huang, Y., Zhang, Y., Farag, M. A., & Zhao, C. (2023). Regulatory mechanisms of the edible alga Ulva lactuca polysaccharide via modulation of gut microbiota in diabetic mice. Food Chemistry, 409, Article 135287.
https://doi.org/10.1016/j.foodchem.2022.135287 - Ruggeri, L. M., Maffei, C., Prisa, D., Crea, F., & Spagnuolo, D. (2026). Eco-friendly antifouling coatings based on macroalgal extracts from Ulva ohnoi and Asparagopsis taxiformis. Clean Technologies, 8(1), Article 8.
https://doi.org/10.3390/cleantechnol8010008 - Shobier, A. H., Ismail, M. M., & Hassan, S. W. M. (2023). Variation in anti-inflammatory, anti-arthritic, and antimicrobial activities of different extracts of common Egyptian seaweeds with an emphasis on their phytochemical and heavy metal contents. Biological Trace Element Research, 201(4), 2071–2087.
https://doi.org/10.1007/s12011-022-03297-1 - Silva, M., Vieira, L. M. M., Almeida, A. P., Silva, A. M. S., Seca, A. M. L., Barreto, M. C., Neto, A. I, Pedro, M., Pinto, E., & Kijjoa, A. (2013). Chemical study and biological activity evaluation of two Azorean macroalgae: Ulva rigida and Gelidium microdon. Oceanography, 1(1), 1–7.
https://doi.org/10.4172/ocn.1000102 - Simon, C., McHale, M., & Sulpice, R. (2022). Applications of Ulva Biomass and Strategies to Improve Its Yield and Composition: A Perspective for Ulva Aquaculture. Biology, 11(11), 1593.
https://doi.org/10.3390/biology11111593 - Sohail, N., Azam, M., Farhat, H., Hira, K., Urooj, F., Qureshi, S. A., Ara, J., Ali, M. S., & Ehteshamul-Haque, S. (2024). Ulva fasciata, a green alga, attenuates the kidney and liver dysfunctions in rats induced by acetaminophen. Drug and Chemical Toxicology, 47(1), 1–14.
https://doi.org/10.1080/01480545.2022.2150206 - Spagnuolo, D., Di Martino, A., Zammuto, V., Armeli Minicante, S., Spanò, A., Manghisi, A., Gugliandolo, C., Morabito, M., & Genovese, G. (2022). Conventional vs. innovative protocols for the extraction of polysaccharides from macroalgae. Sustainability, 14(10), Article 5750.
https://doi.org/10.3390/su14105750 - Steinhagen, S., Larsson, K., Olsson, J., Albers, E., Undeland, I., Pavia, H., & Toth, G. B. (2022). Closed life-cycle aquaculture of sea lettuce (Ulva fenestrata): Performance and biochemical profile differ in early developmental stages. Frontiers in Marine Science, 9, Article 942679.
https://doi.org/10.3389/fmars.2022.942679 - Torres, P., Osaki, S., Silveira, E., dos Santos, D. Y., & Chow, F. (2024). Comprehensive evaluation of Folin-Ciocalteu assay for total phenolic quantification in algae (Chlorophyta, Phaeophyceae, and Rhodophyta). Algal Research, 80, Article 103503.
https://doi.org/10.1016/j.algal.2024.103503 - Trentin, R., Custódio, L., Rodrigues, M. J., Moschin, E., Sciuto, K., da Silva, J. P., & Moro, I. (2020). Exploring Ulva australis Areschoug for possible biotechnological applications: In vitro antioxidant and enzymatic inhibitory properties, and fatty acids contents. Algal Research, 50, Article 101980.
https://doi.org/10.1016/j.algal.2020.101980 - Unnikrishnan, P. S., Animish, A., Madhumitha, G., Suthindhiran, K., & Jayasri, M. A. (2022). Bioactivity guided study for the isolation and identification of antidiabetic compounds from edible seaweed—Ulva reticulata. Molecules (Basel, Switzerland), 27(24), Article 8827.
https://doi.org/10.3390/molecules27248827 - Vyas, A., Jain, V., Sahu, U., Kumar, N., & Joshi, N. (2023). HPTLC method development of herbal drugs and its validation: An overview. Research Journal of Pharmacy and Technology, 16(8), 3964–3976.
https://doi.org/10.52711/0974-360X.2023.00652 - Wan, Y., Liu, L., Zhang, B., Wang, S., Wang, X., Chen, K., Zhao, T., & Qi, H. (2022). Structural characterization and anti-nonalcoholic fatty liver effect of high-sulfated Ulva pertusa polysaccharide. Pharmaceuticals (Basel, Switzerland), 16(1), Article 62.
https://doi.org/10.3390/ph16010062 - Yao, L., Liang, Y., Sun, M., Song, S., Wang, H., Dong, Z., Feng, T., & Yue, H. (2022). Characteristic volatile fingerprints of three edible marine green algae (Ulva spp.) in China by HS-GC-IMS and evaluation of the antioxidant bioactivities. Food Research International (Ottawa, Ont.), 162(Pt B), Article 112109.
https://doi.org/10.1016/j.foodres.2022.112109 - Yi, L., Wang, Q., Luo, H., Lei, D., Tang, Z., Lei, S., & Xiao, H. (2022). Inhibitory effects of polyphenols-rich components from three edible seaweeds on inflammation and colon cancer in vitro. Frontiers in Nutrition, 9, Article 892312.
https://doi.org/10.3389/fnut.2022.856273 - Zaatout, H. H., Ghareeb, D. A., Abd-Elgwad, A., & Ismael, A. A. (2019). Phytochemical, antioxidant, and anti-inflammatory screening of the Egyptian Ulva lactuca methanolic extract. Records of Pharmaceutical and Biomedical Sciences, 3(2), 33–38.
https://doi.org/10.21608/rpbs.2019.12251.1032
Language: English
Page range: 300 - 314
Submitted on: May 15, 2026
Accepted on: Aug 7, 2026
Published on: Sep 9, 2026
Published by: University of Gdańsk
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Related subjects:
© 2026 Lorenzo Maria Ruggeri, Domenico Prisa, Damiano Spagnuolo, published by University of Gdańsk
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.