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Preliminary study on antimicrobial activities of skin mucus from by-catch of Elasmobranch species Cover

Preliminary study on antimicrobial activities of skin mucus from by-catch of Elasmobranch species

Oceanological and Hydrobiological Studies
Volume 52 (2023): Issue 2 (June 2023)
By: Elif Özlem Arslan Aydoğdu,  Mert Kesiktaş,  Nazmiye Ozlem Sanli,  Nihal Doğruöz Güngör,  Serap Sancar,  Taner Yildiz and  Emre Yemişken  
Open Access
|Jun 2023

Figures & Tables

Figure 1

The effects of D. pastinaca mucus on cell viability of 3T3 fibroblast cells. Each bar represents the mean ± SEM of three independent experiments. The *p < 0.05, **p < 0.01, ***p < 0.001 versus control cells.
The effects of D. pastinaca mucus on cell viability of 3T3 fibroblast cells. Each bar represents the mean ± SEM of three independent experiments. The *p < 0.05, **p < 0.01, ***p < 0.001 versus control cells.

Figure 2

The effects of M. aquila mucus on cell viability of 3T3 fibroblast cells. Each bar represents the mean ± SEM of three independent experiments. The *p < 0.05 versus control cells.
The effects of M. aquila mucus on cell viability of 3T3 fibroblast cells. Each bar represents the mean ± SEM of three independent experiments. The *p < 0.05 versus control cells.

Figure 3

The effects of R. clavata mucus on cell viability of 3T3 fibroblast cells. Each bar represents the mean ± SEM of three independent experiments. The *p < 0.05, **p < 0.01, ***p < 0.001 versus control cells.
The effects of R. clavata mucus on cell viability of 3T3 fibroblast cells. Each bar represents the mean ± SEM of three independent experiments. The *p < 0.05, **p < 0.01, ***p < 0.001 versus control cells.

Figure 4

SDS-PAGE of mucus and skin extracts of R. clavata, D. pastinaca, and M. aquila. 40 μg mucoproteins of each sample were denaturated, loaded and separated on the 12% SDS-PAGE gels under 120 volts. The gels were fixed and then stained in 0.1% (v/v) Coomassie blue R-250. The weight of each protein band was calculated according to the weight of known proteins in the standard (S) loaded into the first well.
SDS-PAGE of mucus and skin extracts of R. clavata, D. pastinaca, and M. aquila. 40 μg mucoproteins of each sample were denaturated, loaded and separated on the 12% SDS-PAGE gels under 120 volts. The gels were fixed and then stained in 0.1% (v/v) Coomassie blue R-250. The weight of each protein band was calculated according to the weight of known proteins in the standard (S) loaded into the first well.

Growth inhibition rates (%) of tested strains by the acidic mucus extract of R_ clavata

Test microorganismsThe concentration of mucus extracts (μg μl-1)Negative Control (cfu ml-1)
502512.56.25
E. coli ATCC 25922> 99.999> 99.699> 99.999> 99.9992.23 × 1012
S. aureus ATCC 65382.57 × 1011
P. aeruginosa ATCC 902795.10094.8692.98 × 1013
MRSA ATCC 3359141.53929.0391.24 × 1011
VRE ATCC 5129999.46998.79963.17932.2791.89 × 1012
C. albicans ATCC 10231> 99.999> 99.999> 99.999> 99.9996.90 × 1011

Isolates from epidermal mucus of fish species and their rates of antimicrobial activity

SpeciesNumber of colony (cfu ml-1)Number of isolatesAntimicrobial activity rates of isolates (%)
Dasyatis pastinaca2442.5 ± 67.4640
Myliobatis aquila3665 ± 123.2782
Raja clavata37.5 ± 1.8225

Growth inhibition rates (%) of tested strains by the acidic mucus extract of D_ pastinaca

Test MicroorganismsThe concentration of mucus extracts (μg μl-1)Negative Control (cfu ml-1)
502512.56.25
E. coli ATCC 2592273.72960.16943.0197.6097.23 × 1013
S. aureus ATCC 653899.14985.40960.57952.1592.09 × 1013
P. aeruginosa ATCC 9027> 99.859> 92.47943.69937.3992.38 × 1014
MRSA ATCC 33591> 99.939> 99.60999.22998.0893.87 × 1014
VRE ATCC 5129991.24975.46924.499NR2.49 × 1010
C. albicans ATCC 10231> 99.999> 99.999> 99.999> 99.9698.35 × 1011

Growth inhibition rates (%) of tested strains by the acidic mucus extract of M_ Aquila

Test MicroorganismsThe concentration of mucus extracts (μg μl-1)Negative Control (cfu ml-1)
502512.56.25
E. coli ATCC 2592299.93999.90999.85978.5697.23 × 1013
S. aureus ATCC 653899.77997.62984.49978.8092.09 × 1013
P. aeruginosa ATCC 9027> 97.599> 94.66990.88989.0792.38 × 1014
MRSA ATCC 33591> 96.539> 92.79989.76987.1893.87 × 1014
VRE ATCC 5129999.95998.579NRNR2.49 × 1010
C. albicans ATCC 10231> 99.999> 99.999> 99.999> 99.9898.35 × 1011
DOI: https://doi.org/10.26881/oahs-2023.2.01 | Journal eISSN: 1897-3191 | Journal ISSN: 1730-413X
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Language: English
Page range: 137 - 146
Submitted on: Jun 13, 2022
Accepted on: Mar 8, 2023
Published on: Jun 28, 2023
Published by: University of Gdańsk
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
fish skin mucus,
antimicrobial activity,
batoid,
skate,
ray
Related subjects:
Chemistry,
Chemistry, other,
Geosciences,
Geosciences, other,
Life sciences,
Life sciences, other

© 2023 Elif Özlem Arslan Aydoğdu, Mert Kesiktaş, Nazmiye Ozlem Sanli, Nihal Doğruöz Güngör, Serap Sancar, Taner Yildiz, Emre Yemişken, published by University of Gdańsk
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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