Effects of chokeberry pomace supplementation on biochemical, metabolic and antioxidant parameters in high-yielding dairy goats

Journal of Veterinary Research

Volume 69 (2025): Issue 3 (September 2025)

By:

Monika Szymańska-Czerwińska

, Krzysztof Niemczuk

, Nina Strzałkowska

, Zbigniew Osiński

, Agnieszka Wierzbicka

, Barbara Wijas

, Sylwester Marczak

, Karina Horbańczuk

and Artur Jóźwik

Open Access

|Oct 2025

Figures & Tables

Pearson correlation matrix of selected biochemical, enzymatic, antioxidant and metabolic parameters in high-yielding dairy goats after supplementation with Arona melanocarpa pomace

Lysosomal enzyme activity in blood serum of lactating goats after supplementation with Aronia melanocarpa pomace

Parameter	Pomace mass per kg feed (group)	Mean ± standard deviation		P-value
		Start	Finish	A1, A2, C
		Start	Finish	Start to finish
ACPnmol/(mg protein·h)	15g (A1)	10.9±1.40	15.9±2.23	<0.001*
	30g (A2)	10.7±1.02	19.4±1.28	<0.001*
	control (C)	11.0±1.1	11.3±0.93	0.662
β-GRDnmol/(mg protein·h)	15g (A1)	2.3±0.35	2.0±0.30	0.052
	30g (A2)	2.4±0.47	1.7±0.25	<0.001*
	control (C)	2.4±0.2	2.3±0.22	0.468
β-GALnmol/(mg protein·h)	15g (A1)	3.4±0.29	4.2±0.26	<0.001*
	30g (A2)	3.4±0.50	4.8±0.32	<0.001*
	control (C)	3.5±0.4	3.6±0.34	0.429
β-GLUnmol/(mg protein·h)	15g (A1)	1.9±0.14	4.7±0.62	<0.001*
	30g (A2)	2.1±0.24	5.2±0.64	<0.001*
	control (C)	2.1±0.3	2.1±0.22	0.856
HEXnmol/(mg protein·h)	15g (A1)	2.9±0.42	5.4±0.51	<0.001*
	30g (A2)	2.6±0.42	5.8±0.78	<0.001*
	control (C)	2.8±0.5	2.8±0.35	0.871
α-GLUnmol/(mg protein·h)	15g (A1)	7.5±0.92	5.5±0.58	<0.001*
	30g (A2)	8.0±0.63	5.1±0.49	<0.001*
	control (C)	7.9±0.7	7.4±0.38	0.111
MANnmol/(mg protein·h)	15g (A1)	7.6±0.97	6.5±0.76	0.004*
	30g (A2)	7.6±0.43	6.6±0.69	0.013*
	control (C)	7.7±0.9	7.3±0.84	0.279

Metabolic and renal function parameters in blood serum of lactating goats after supplementation with Aronia melanocarpa pomace

Parameter	Pomace mass per kg feed (group)	Mean ± standard deviation		P-value
Parameter	Pomace mass per kg feed (group)	Start	Finish	A1, A2, CStart to finish
ALBg/L	15g (A1)	41.7±3.82	38.5±2.51	0.044*
	30g (A2)	40.1±1.87	39.0±1.29	0.483
	control (C)	42.2±5.6	37.6±3.36	0.006*
GGTIU/L	15g (A1)	51.5±5.68	45.4±4.78	0.012*
	30g (A2)	51.7±3.40	41.3±4.12	<0.001*
	control (C)	59.2±5.3	54.2±6.21	0.042*
ALPIU/L	15g (A1)	124.7±43.69	99.4±11.96	0.046*
	30g (A2)	110.1±26.08	115.7±23.78	0.645
	control (C)	119.2±27.0	102.0±6.95	0.184
ALTIU/L	15g (A1)	17.2±2.05	16.3±1.94	0.364
	30g (A2)	16.4±1.59	14.9±2.08	0.109
	control (C)	18.6±2.4	18.0±2.20	0.581
ASTIU/L	15g (A1)	89.2±8.63	79.7±8.31	0.006*
	30g (A2)	78.1±4.83	74.8±5.69	0.312
	control (C)	91.3±7.2	90.1±7.06	0.726
BILT mg/dl	15g (A1)	0.8±0.20	0.9±0.14	0.310
	30g (A2)	0.7±0.18	0.6±0.19	0.127
	control (C)	0.9±0.2	0.9±0.19	0.975
LIPIU/L	15g (A1)	19.8±2.23	17.4±1.85	0.010*
	30g (A2)	19.1±1.90	16.5±1.81	0.005*
	control (C)	19.8±2.1	19.0±1.57	0.366
CHEIU/L	15g (A1)	73.9±14.51	53.8±8.89	0.001*
	30g (A2)	52.8±13.74	57.0±13.25	0.453
	control (C)	70.2±11.7	55.1±9.14	0.014*
TP g/L	15g (A1)	72.7±5.26	70.2±5.62	0.345
	30g (A2)	71.1±5.41	69.8±4.13	0.610
	control (C)	75.4±6.4	72.3±6.30	0.249
CKIU/L	15g (A1)	183.8±20.81	158.8±20.84	0.077
	30g (A2)	146.6±17.56	214.7±50.78	<0.001*
	control (C)	181.4±29.0	150.0±16.71	0.032*
LDHIU/L	15g (A1)	801.3±50.46	778.2±37.45	0.458
	30g (A2)	779.6±53.64	638.9±48.52	<0.001*
	control (C)	798.9±106.6	810.6±75.38	0.714
CREμmol/L	15g (A1)	60.0±6.26	59.3±3.02	0.761
	30g (A2)	64.2±3.70	61.7±6.09	0.255
	control (C)	61.7±3.6	57.3±4.24	0.062
GLUmmol/L	15g (A1)	3.1±0.41	3.1±0.18	0.609
	30g (A2)	3.2±0.22	3.4±0.31	0.266
	control (C)	3.1±0.4	3.3±0.24	0.289
LACTmmol/L	15g (A1)	2.3±0.17	1.5±0.21	<0.001*
	30g (A2)	2.6±0.47	1.6±0.31	<0.001*
	control (C)	2.7±0.6	1.7±0.38	<0.001*

Macro- and microelement concentrations in blood serum of lactating goats after supplementation with Aronia melanocarpa pomace

Parameter	Pomace mass per kg feed (group)	Mean ± standard deviation		P-value
Parameter	Pomace mass per kg feed (group)	Start	Finish	A1, A2, CStart to finish
Kmmol/L	15g (A1)	4.3±0.39	4.2±0.34	0.726
	30g (A2)	4.2±0.56	4.2±0.32	0.746
	control (C)	4.1±0.4	4.2±0.45	0.707
Nammol/L	15g (A1)	148.5±8.57	134.2±3.16	<0.001*
	30g (A2)	146.3±5.61	137.0±2.71	0.001*
	control (C)	150.3±8.3	137.4±2.57	<0.001*
Clmmol/L	15g (A1)	110.1±7.24	108.9±2.61	0.614
	30g (A2)	107.6±6.50	108.7±2.78	0.624
	control (C)	110.8±6.1	108.1±1.86	0.265
Mgmmol/L	15g (A1)	1.3±0.15	1.1±0.09	<0.001*
	30g (A2)	1.3±0.07	1.2±0.06	0.035*
	control (C)	1.3±0.1	1.2±0.05	0.002*
Pmmol/L	15g (A1)	2.8±0.33	2.3±0.30	0.010*
	30g (A2)	2.2±0.19	2.2±0.32	0.786
	control (C)	2.9±0.4	2.8±0.47	0.815
Cammol/L	15g (A1)	2.0±0.34	2.0±0.16	0.488
	30g (A2)	2.1±0.15	2.2±0.11	0.696
	control (C)	2.1±0.4	2.0±0.30	0.511
Feμmol//L	15g (A1)	21.5±1.29	26.9±2.47	<0.001*
	30g (A2)	20.5±1.31	30.9±1.74	<0.001*
	control (C)	20.7±1.4	21.8±2.58	0.210

Antioxidant parameters in blood serum of lactating goats after supplementation with Aronia melanocarpa pomace

Parameter	Pomace mass per kg feed (group)	Mean ± standard deviation		P-value
		Start	Finish	A1, A2, C
		Start	Finish	Start to finish
DPPH% inhibition	15g (A1)	60.1±3.47	62.3±1.60	0.054
	30g (A2)	59.8±1.65	68.7±2.67	< 0.001*
	control (C)	60.2±2.4	60.8±1.10	0.630
Vitamin Cmg/100 mL	15g (A1)	0.9±0.12	1.1±0.11	0.039*
	30g (A2)	1.0±0.15	1.1±0.08	0.136
	control (C)	0.9±0.2	0.9±0.12	0.637
GSHμmol	15g (A1)	283.8±46.35	391.3±67.51	< 0.001*
	30g (A2)	284.3±19.57	509.2±44.12	< 0.001*
	control (C)	283.5±32.3	279.6±38.10	0.856
POLmg GAE/mL	15g (A1)	1.6±0.30	1.6±0.14	0.844
	30g (A2)	1.6±0.32	1.9±0.23	0.013*
	control (C)	1.6±0.2	1.6±0.27	0.834

Aminopeptidase enzyme activity in blood serum of lactating goats after supplementation with Aronia melanocarpa pomace

Parameter	Pomace mass per kg feed (group)	Mean ± standard deviation		P-value
		Start	Finish	A1, A2, C
		Start	Finish	Start to finish
AlaAPnmol/(mg protein·h)	15g (A1)	4.1±0.36	4.2±0.40	0.727
	30g (A2)	4.0±0.44	4.5±0.48	0.007*
	control (C)	4.3±0.4	4.3±0.38	0.809
LeuAPnmol/(mg protein·h)	15g (A1)	3.0±0.45	2.9±0.24	0.541
	30g (A2)	2.9±0.45	2.7±0.41	0.295
	control (C)	3.0±0.3	2.6±0.26	0.033*
ArgAPnmol/(mg protein·h)	15g (A1)	4.0±0.46	3.5±0.43	0.017*
	30g (A2)	4.8±0.37	4.0±0.44	< 0.001*
	control (C)	4.9±0.4	4.7±0.54	0.381

Lipid profile changes in blood serum of lactating goats after supplementation with Aronia melanocarpa pomace

Parameter	Pomace mass per kg feed (group)	Mean ± standard deviation		P-value
Parameter	Pomace mass per kg feed (group)	Start	Finish	A1, A2, CStart to finish
TRIG mmol/L	15g (A1)	Start	Finish	0.3±0.05	0.2±0.04	0.014*
	30g (A2)	0.3±0.03	0.2±0.03	<0.001*
	control (C)	0.3±0.1	0.4±0.06	0.123
CHOLmmol/L	15g (A1)	3.0±0.47	2.7±0.31	0.076
	30g (A2)	3.0±0.22	2.6±0.37	0.011*
	control (C)	3.2±0.4	3.3±0.38	0.467
HDLmmol/L	15g (A1)	2.1±0.17	2.3±0.13	0.006*
	30g (A2)	2.1±0.35	2.6±0.14	<0.001*
	control (C)	2.1±0.1	2.0±0.17	0.313
LDLmmol/L	15g (A1)	0.8±0.06	0.7±0.10	0.011
	30g (A2)	0.8±0.09	0.6±0.12	0.005*
	control (C)	0.8±0.1	0.9±0.15	0.242

DOI: https://doi.org/10.2478/jvetres-2025-0054 | Journal eISSN: 2450-8608

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Language: English

Page range: 417 - 427

Submitted on: Jul 11, 2025

Accepted on: Sep 25, 2025

Published on: Oct 4, 2025

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 times per year

Keywords:

bioactives,

biochemical parameters,

Related subjects:

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2025 Monika Szymańska-Czerwińska, Krzysztof Niemczuk, Nina Strzałkowska, Zbigniew Osiński, Agnieszka Wierzbicka, Barbara Wijas, Sylwester Marczak, Karina Horbańczuk, Artur Jóźwik, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

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