Fig. 1
Real-time positive PCR results for the chicken thigh samples used in this study
| Positive | Average | Estimated number | SAG1 PCR |
|---|---|---|---|
| samples | Ct | of copies T. gondii* | product** |
| 1 | 35.30 | 2.1/g | positive |
| 4 | 34.60 | 3.4/g | positive |
| 14 | 36.16 | 1.1/g | positive |
| 15 | 34.31 | 4.1/g | positive |
| 20 | 35.71 | 1.6/g | positive |
Primers utilised for the detection of T_ gondii in this study
| Primer | Primer sequences | Gene | Length (nt) | Reference |
|---|---|---|---|---|
| ToxoRoc F | 5ʹ-TAGACGAGACGACGCTTTCC-3ʹ | T. gondii repetitive | 64 | (11) |
| ToxoRoc R | 5ʹ- TCGCCCTCTTCTCCACTCT-3ʹ | sequence | ||
| ToxoRepeat 500 F | 5ʹ-CGCTGCAGGGAGGAAGACGAAAGTTG-3ʹ | T. gondii repetitive | ||
| ToxoRepeat 500 R | 5ʹ-CGCTGCAGACACAGTGCATCTGGATT-3ʹ | sequence | 529 | (11) |
| ToxoSG1 F | 5ʹ-TCATCGGTCGTCAATAA-3ʹ | |||
| ToxoSG1 R | 5ʹ- CTTTGACTCCATCTTTCC-3ʹ | SAG1 | 123 | (11) |