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Characterisation and antibiotic susceptibility profile of Clostridioides (Clostridium) difficile isolated from chicken carcasses

Journal of Veterinary Research

Volume 64 (2020): Issue 3 (September 2020)

By: Enver Baris Bingol, Hamparsun Hampikyan, Karlo Muratoglu, Esra Akkaya, Omer Cetin and Hilal Colak

Open Access

|Aug 2020

Figures & Tables

Primer sequence list used in the study

Gene	Primers	Amplicon size	Reference
tpi	F: 5′-AAAGAAGCTACTAAGGGTACAAA-3′	230 bp	15
tpi	R: 5′-CATAATATTGGGTCTATTCCTAC-3′	230 bp	15

tcdA	F: 5′-AGATTCCTATATTTACATGACAATAT-3′	369 bp	15
tcdA	R: 5′-GTATCAGGCATAAAGTAATATACTTT-3′	369 bp	15

tcdB	F: 5′-GGAAAAGAGAATGGTTTTATTAA-3′	160 bp	15
tcdB	R: 5′-ATCTTTAGTTATAACTTTGACATCTTT-3′	160 bp	15

cdtA	F: 5′-TGAACCTGGAAAAGGTGATG-3′	353 bp	28
cdtA	R: 5′-AGGATTATTTACTGGACCATTTG-3′	353 bp	28

cdtB	F: 5′-CTTATTGCAAGTAAATACTGAGAGTACTATATC-3′	490 bp	28
cdtB	R: 5′-ACCGGATCTCTTGCTTCAGTC-3′	490 bp	28

The distribution of determined ribotypes in terms of antibiotic susceptibility

		AMP			AMC			DA			IMP			MTZ			TE			VA			CTX
RTs	n	S	I	R	S	I	R	S	I	R	S	I	R	S	I	R	S	I	R	S	I	R	S I	R
RT027	6	4	2	-	6	-	-	4	1	1	-	-	6	6	-	-	6	-	-	6	-	-	- -	6
RT087	4	3	1	-	4	-	-	3	1	-	-	-	4	4	-	-	4	-	-	4	-	-	- -	4
RT470	4	2	2	-	4	-	-	4	-	-	-	-	4	4	-	-	4	-	-	4	-	-	- -	4
RT085	4	4	-	-	4	-	-	3	1	-	-	-	4	4	-	-	4	-	-	4	-	-	- -	4
RT456	2	2	-	-	2	-	-	2	-	-	-	-	2	2	-	-	2	-	-	2	-	-	- -	2
RT020	2	2	-	-	2	-	-	1	1	-	-	-	2	2	-	-	2	-	-	2	-	-	- -	2
RT010	1	1	-	-	1	-	-	1	-	-	-	-	1	1	-	-	1	-	-	1	-	-	- -	1
RT003	1	1	-	-	1	-	-	1	-	-	-	-	1	1	-	-	1	-	-	1	-	-	- -	1
ML027	6	3	3	-	6	-	-	3	2	1	-	-	6	6	-	-	6	-	-	6	-	-	- -	6
NR	3	26	11	2	39	-	-	17	8	14	6	1	32	31	-	8	36	1	2	37	-	2	2 -	3
	9																							7

TOTAL	69	48	19	2	69	-	-	39	14	16	6	1	62	61	-	8	66	1	2	67	-	2	2 -	67

Susceptibility profiles of C_ difficile isolates from chicken carcasses

n	Susceptibility	AMP (%)	AMC (%)	DA (%)	IMP (%)	MTZ (%)	TE (%)	VA (%)	CTX (%)
	Susceptible	48	69	39	6	61	66	67	2
		(69.6)	(100)	(56.5)	(8.7)	(88.4)	(95.7)	(97.1)	(2.9)
		19	0	14	1	0	1	0	0
69	Intermediate	(27.5)	(0)	(20.3)	(1.4)	(0)	(1.4)	(0)	(0)
		2	0	16	62	8	2	2	67
	Resistant	(2.9)	(0)	(23.2)	(89.9)	(11.6)	(2.9)	(2.9)	(97.1)

The distribution of the virulence genes and ribotypes of C_ difficile isolates (n = 69)

Toxigenic genes			Samples (%)	Ribotypes (n^r)
tcdA⁺	tcdB⁺	cdtA/B⁺	17 (24.6)	027 (6), 003 (1), ML^#027 (6), NR^* (4)
tcdA⁺	tcdB⁺	cdtA/B⁻	14 (20.3)	087 (4), 020 (2), NR (8)
tcdA⁺	tcdB⁻	cdtA/B⁺	1 (1.5)	NR (1)
tcdA⁺	tcdB⁻	cdtA/B⁻	3 (4.4)	NR (3)
tcdA⁻	tcdB⁺	cdtA/B⁺	13 (18.8)	NR (13)
tcdA⁻	tcdB⁺	cdtA/B⁻	11 (15.9)	470 (4), 456 (2), NR (5)
tcdA⁻	tcdB⁻	cdtA/B⁺	0 (0)	ND^**
tcdA⁻	tcdB⁻	cdtA/B⁻	10 (14.5)	010 (1), 085 (4), NR (5)

DOI: https://doi.org/10.2478/jvetres-2020-0052 | Journal eISSN: 2450-8608

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Language: English

Page range: 407 - 412

Submitted on: Jan 16, 2020

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Accepted on: Jul 27, 2020

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Published on: Aug 11, 2020

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

antibiotic susceptibility,

Related subjects:

Molecular biology,

Microbiology and virology,

Life sciences, other,

Veterinary medicine

© 2020 Enver Baris Bingol, Hamparsun Hampikyan, Karlo Muratoglu, Esra Akkaya, Omer Cetin, Hilal Colak, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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