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High-Throughput Resistance Phenotyping of Banana (Musa spp.) against Radopholus similis Cover

High-Throughput Resistance Phenotyping of Banana (Musa spp.) against Radopholus similis

Journal of Nematology
Volume 57 (2025): Issue 1 (February 2025)
By: Joseph Kisitu,  Kanan K. Saikai,  Danny Coyne,  Reagan Kanaabi,  James Kisaakye,  Rony Swennen and  Gloria V. Nakato  
Open Access
|Dec 2025

Figures & Tables

Figure 1:

Schematic representation of the methodology used in preparation of modified MS media, nematode inoculation and extraction. MS, Murashige and Skoog, TC, tissue-culture.
Schematic representation of the methodology used in preparation of modified MS media, nematode inoculation and extraction. MS, Murashige and Skoog, TC, tissue-culture.

Figure 2:

Contamination intensity (A) in banana tissue-cultured plants at 8 wk post-infection with 50 female R. similis sterilized with either SDW or streptomycin sulfate solution. Data pooled across two experiments and four banana accessions. A bar graph with different letters indicates a significant difference. Means separated by Tukey's HSD test at P < 0.05; Uncontaminated (B) and contaminated (C) plants at 8 wk post infection with R. similis sterilized with SDW and streptomycin sulfate solution, respectively. HSD, honestly significant difference; SDW, sterile distilled water.
Contamination intensity (A) in banana tissue-cultured plants at 8 wk post-infection with 50 female R. similis sterilized with either SDW or streptomycin sulfate solution. Data pooled across two experiments and four banana accessions. A bar graph with different letters indicates a significant difference. Means separated by Tukey's HSD test at P < 0.05; Uncontaminated (B) and contaminated (C) plants at 8 wk post infection with R. similis sterilized with SDW and streptomycin sulfate solution, respectively. HSD, honestly significant difference; SDW, sterile distilled water.

Figure 3:

Reproduction of R. similis in roots of control banana accessions at 2 wk (A), 4 wk (B), and 8 wk (C) post nematode inoculation under in vitro conditions. At each time point, means followed by different letter(s) indicate a significant difference. Means separated by Tukey's HSD test at P < 0.05. HSD, honestly significant difference; RF, reproduction factor.
Reproduction of R. similis in roots of control banana accessions at 2 wk (A), 4 wk (B), and 8 wk (C) post nematode inoculation under in vitro conditions. At each time point, means followed by different letter(s) indicate a significant difference. Means separated by Tukey's HSD test at P < 0.05. HSD, honestly significant difference; RF, reproduction factor.

Figure 4:

Forest plot comparing nematode reproduction (RF) in banana accessions under in vitro and screenhouse conditions. For each accession, square markers represent the mean RF value, with horizontal standard error bars. The vertical dotted red line indicates the highest RF value at which no significant difference (P > 0.05) from the resistant check is observed. RF, reproduction factor.
Forest plot comparing nematode reproduction (RF) in banana accessions under in vitro and screenhouse conditions. For each accession, square markers represent the mean RF value, with horizontal standard error bars. The vertical dotted red line indicates the highest RF value at which no significant difference (P > 0.05) from the resistant check is observed. RF, reproduction factor.

Response of TC banana accessions to R_ similis infection_

AccessionGenome groupResistance response
cv. RoseAAUnknown
TMB2x 9172AAUnknown
SH3142AAResistant
PahangAAUnknown
PITA 7AAABUnknown
PITA 6AAABUnknown
Obino l'EwaiAABSusceptible
MchareAASusceptible
MbwazirumeEA-AAASusceptible
IITA hybrid 2145/1320AAUnknown
716AAUnknown
401K-1AAAAUnknown
376K-1AAAAUnknown
365K-1AAAAUnknown
29275S-5AAAAUnknown
201087K-4AAUnknown
201071KAAUnknown
10969S-1AAUnknown
Yangambi km5AAAResistant
ValeryAAASusceptible
DOI: https://doi.org/10.2478/jofnem-2025-0055 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: May 27, 2025
|
Published on: Dec 7, 2025
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
banana nematodes,
lesion nematode,
rapid screening,
resistance screening
Related subjects:
Life sciences,
Life sciences, other

© 2025 Joseph Kisitu, Kanan K. Saikai, Danny Coyne, Reagan Kanaabi, James Kisaakye, Rony Swennen, Gloria V. Nakato, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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