First Record of Mermithidae (Enoplea: Mermithida) Parasitizing Philaenus spumarius (Hemiptera: Aphrophoridae) in Central Italy

Figures & Tables

		*Philaenus spumarius*		Mermithidae
Site	Coordinates	No. of dissected specimens	Examined instar	No. of observed nematodes	No. of nematodes per spittlebug	Parasitization rate (%)	Species
A^a	43.938753N	411	n1, n2, n3, n4, n5, adult	94	1–4	22.87	1
A^a	11.141433E	411	n1, n2, n3, n4, n5, adult	94	1–4	22.87	1
B	43.811145N	516	n2, n3, n4, n5, adult	17	1	3.29	1
B	11.031118E	516	n2, n3, n4, n5, adult	17	1	3.29	1
C^a	43.732465N	322	n1, n2, n3, n4, n5, adult	16	1–2	4.97	2
C^a	11.254696E	322	n1, n2, n3, n4, n5, adult	16	1–2	4.97	2
D^a	43.66854N	436	n1, n2, n3, n4, n5, adult	81	1–9	18.58	2
D^a	11.152888E	436	n1, n2, n3, n4, n5, adult	81	1–9	18.58	2
E^a	43.799643N	322	n1, n2, n3, n4, n5, adult	17	1–5	5.28	2
E^a	11.403262E	322	n1, n2, n3, n4, n5, adult	17	1–5	5.28	2
F	43.761272N	402	n1, n2, n3, n4, n5, adult	0		-	-
F	10.452208E	402	n1, n2, n3, n4, n5, adult	0		-	-
G	42.737900N	400	n2, n3, n4, n5, adult	0		-	-
G	11.05498E	400	n2, n3, n4, n5, adult	0		-	-
H	43.508942N	341	n1, n2, n3, n4, adult	0		-	-
H	11.874063E	341	n1, n2, n3, n4, adult	0		-	-

Primer name	Primer sequence	T_a	Reference
988F	CTCAAAGATTAAGCCATGC	45.0 °C	Holterman et al., 2006
26R	CATTCTTGGCAAATGCTTTCG		Nguyen and Hunt, 2007
MermF	CAAGGACGAAAGTTAGAGGTTC	47.0 °C	Kobylinski et al., 2012
MermR	GGAAACCTTGTTACGACTTTTA
Nem1	GCAAGTCTGGTGCCAGCAGC	45.0 °C	Foucher and Wilson, 2002
Nem2	CCGTGTTGAGTCAAATTAAG
18S	TTGATTACGTCCCTGCCCTTT	50.0 °C	Vrain et al., 1992
26S	TTTCACTCGCCGTTACTAAGG
D2A	ACAAGTACCGTGAGGGAAAGTTG	52.0 °C	Douda et al., 2010
D3B	TCGGAAGGAACCAGCTACTA