Table 1:
Morphometrics of Ditylenchoides agaricivorus n. sp.
| Character/ration | Female | Male | |
|---|---|---|---|
| Holotype | Paratypes | Paratypes | |
| n | - | 20 | 15 |
| L | 745.58 a | 728 ± 61.3 (612–846) | 641 ± 61.7 (511–720) |
| a | 40.85 | 40.4 ± 2.3 (34.5–43.4) | 41.3 ± 3.1 (34.5–47.1) |
| b | 6.83 | 6.4 ± 0.7 (5.4–8.5) | 5.6 ± 0.4 (4.7–6.2) |
| c | 10.82 | 11.1 ± 0.7 (9.9–12.7) | 10.6 ± 0.9 (9.1–12.0) |
| c’ | 5.28 | 5.5 ± 0.6 (4.5–6.8) | 5.7 ± 0.6 (4.7–6.5) |
| V or T (%) | 82.57 | 80.0 ± 1.8 (75.5–82.6) | 37.3 ± 3.0 (34.0–43.1) |
| Lip region height | 2.22 | 2.3 ± 0.1 (2.0–2.7) | 1.9 ± 0.2 (1.5–2.2) |
| Lip region diam. | 5.83 | 5.8 ± 0.3 (5.4–6.4) | 5.1 ± 0.3 (4.5–5.6) |
| Stylet length | 8.14 | 8.0 ± 0.3 (7.4–8.4) | 7.6 ± 0.3 (7.1–8.2) |
| Conus length | 2.95 | 2.6 ± 0.3 (2.1–3.0) | 2.6 ± 0.3 (2.2–3.1) |
| m | 36.24 | 32.9 ± 3.4 (26.7–38.1) | 34.0 ± 3.7 (28.8–39.6) |
| Body diam. | 18.25 | 18.1 ± 2.5 (14.1–24.3) | 15.6 ± 1.8 (12.7–19.4) |
| Nerve ring from anterior | 72.7 | 73.6 ± 5.6 (62.9–85.0) | 69.8 ± 3.1 (65.5–75.7) |
| Excretory pore form anterior | 83.67 | 94.3 ± 5.0 (83.7–108.2) | 88.9 ± 6.0 (71.9–98.7) |
| Esophagus length | 109.17 | 113.6 ± 8.3 (91.0–127.6) | 114.4 ± 4.1 (107.0–119.3) |
| Vulva to anus distance (VA) | 80.5 | 79.1 ± 7.5 (66.7–90.3) | - |
| Post vulval uterine sac | 40.76 | 36.5 ± 7.4 (21.8–51.6) | - |
| PUS/VA (%) | 50.63 | 46.0 ± 7.6 (32.7–69.4) | - |
| Ovary or testis length | 266.18 | 254.6 ± 22.6 (217.7–303.6) | 238.8 ± 24.1 (190.3–287.8) |
| Anal (cloacal) body diam. | 13.05 | 12.0 ± 2.0 (9.6–16.8) | 10.8 ± 1.2 (9.0–12.9) |
| Tail length | 68.92 | 65.5 ± 5.4 (58.2–76.9) | 60.8 ± 4.3 (49.9–68.0) |
| Spicule length (arc line) | - | - | 15.4 ± 0.6 (14.7–16.7) |
| Gubernaculum length | - | - | 5.3 ± 0.4 (4.4–6.0) |
Figure 1:
The line drawing of Ditylenchoides agaricivorus n. sp. A: Female entire body; B: Male entire body; C: Female esophageal region; D: Lateral field at mid-body; E: Female posterior body region; F: Male posterior region. (Scale bars = 20 μm).
Figure 2:
Light micrographs of Ditylenchoides agaricivorus n. sp. A: Female entire body; B: Male entire body; C, D: Female anterior body region; E: Spermatheca; F Lateral field at mid-body; G: Vulval region and post-vulval uterine sac; H: Female tail region; I: Male tail region. (Scale bars = 20 μm).
Figure 3:
Bayesian tree generated from the D2D3 of 28S rRNA gene dataset of Anguinid nematodes with the GTR + I + G model. Newly obtained sequence in bold letters (Scale bar = expected changes per site).
Figure 4:
Bayesian tree generated from the ITS rRNA gene dataset of Anguinid nematodes with the GTR + I + G model. The newly obtained sequence is in bold letters (Scale bar = expected changes per site).
Table 2:
The average reproduction factor value of Ditylenchoides agaricivorus n. sp. recovered from fungal cultures, alfalfa seedlings, and callused carrot disks.
| Culture | Juvenile individuals | Adult individuals | Reproduction factor |
|---|---|---|---|
| Callused carrot disk | 0 a | 0 | 0 |
| Alfalfa | 0 | 0 | 0 |
| Rhizoctonia solani | 89.33 ± 31 | 143.56 ± 15 | 11.64 ± 2 |
| Lentinula edodes | 0 | 0.78 ± 1 | 0.04 ± 0 |
| Pleurotus eryngii | 0 | 0.56 ± 1 | 0.03 ± 0 |
| Volvariella volvacea | 0 | 1.89 ± 1 | 0.09 ± 0 |
| Agaricus bisporus | 13.22 ± 12 | 35.22 ± 11 | 2.42 ± 1 |
| Agaricus bitorquis | 0 | 1.89 ± 1 | 0.09 ± 0 |