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Morphological and Molecular Characterization of a Novel Fungal-feeding Stem Nematode Ditylenchoides agaricivorus n. sp. (Tylenchida: Anguinidae) from Intercepted Samples Cover

Morphological and Molecular Characterization of a Novel Fungal-feeding Stem Nematode Ditylenchoides agaricivorus n. sp. (Tylenchida: Anguinidae) from Intercepted Samples

Open Access
|Feb 2025

Figures & Tables

Figure 1:

The line drawing of Ditylenchoides agaricivorus n. sp. A: Female entire body; B: Male entire body; C: Female esophageal region; D: Lateral field at mid-body; E: Female posterior body region; F: Male posterior region. (Scale bars = 20 μm).
The line drawing of Ditylenchoides agaricivorus n. sp. A: Female entire body; B: Male entire body; C: Female esophageal region; D: Lateral field at mid-body; E: Female posterior body region; F: Male posterior region. (Scale bars = 20 μm).

Figure 2:

Light micrographs of Ditylenchoides agaricivorus n. sp. A: Female entire body; B: Male entire body; C, D: Female anterior body region; E: Spermatheca; F Lateral field at mid-body; G: Vulval region and post-vulval uterine sac; H: Female tail region; I: Male tail region. (Scale bars = 20 μm).
Light micrographs of Ditylenchoides agaricivorus n. sp. A: Female entire body; B: Male entire body; C, D: Female anterior body region; E: Spermatheca; F Lateral field at mid-body; G: Vulval region and post-vulval uterine sac; H: Female tail region; I: Male tail region. (Scale bars = 20 μm).

Figure 3:

Bayesian tree generated from the D2D3 of 28S rRNA gene dataset of Anguinid nematodes with the GTR + I + G model. Newly obtained sequence in bold letters (Scale bar = expected changes per site).
Bayesian tree generated from the D2D3 of 28S rRNA gene dataset of Anguinid nematodes with the GTR + I + G model. Newly obtained sequence in bold letters (Scale bar = expected changes per site).

Figure 4:

Bayesian tree generated from the ITS rRNA gene dataset of Anguinid nematodes with the GTR + I + G model. The newly obtained sequence is in bold letters (Scale bar = expected changes per site).
Bayesian tree generated from the ITS rRNA gene dataset of Anguinid nematodes with the GTR + I + G model. The newly obtained sequence is in bold letters (Scale bar = expected changes per site).

The average reproduction factor value of Ditylenchoides agaricivorus n_ sp_ recovered from fungal cultures, alfalfa seedlings, and callused carrot disks_

CultureJuvenile individualsAdult individualsReproduction factor
Callused carrot disk0 a00
Alfalfa000
Rhizoctonia solani89.33 ± 31143.56 ± 1511.64 ± 2
Lentinula edodes00.78 ± 10.04 ± 0
Pleurotus eryngii00.56 ± 10.03 ± 0
Volvariella volvacea01.89 ± 10.09 ± 0
Agaricus bisporus13.22 ± 1235.22 ± 112.42 ± 1
Agaricus bitorquis01.89 ± 10.09 ± 0

Morphometrics of Ditylenchoides agaricivorus n_ sp_

Character/rationFemaleMale


HolotypeParatypesParatypes
n-2015
L745.58 a728 ± 61.3 (612–846)641 ± 61.7 (511–720)
a40.8540.4 ± 2.3 (34.5–43.4)41.3 ± 3.1 (34.5–47.1)
b6.836.4 ± 0.7 (5.4–8.5)5.6 ± 0.4 (4.7–6.2)
c10.8211.1 ± 0.7 (9.9–12.7)10.6 ± 0.9 (9.1–12.0)
c’5.285.5 ± 0.6 (4.5–6.8)5.7 ± 0.6 (4.7–6.5)
V or T (%)82.5780.0 ± 1.8 (75.5–82.6)37.3 ± 3.0 (34.0–43.1)
Lip region height2.222.3 ± 0.1 (2.0–2.7)1.9 ± 0.2 (1.5–2.2)
Lip region diam.5.835.8 ± 0.3 (5.4–6.4)5.1 ± 0.3 (4.5–5.6)
Stylet length8.148.0 ± 0.3 (7.4–8.4)7.6 ± 0.3 (7.1–8.2)
Conus length2.952.6 ± 0.3 (2.1–3.0)2.6 ± 0.3 (2.2–3.1)
m36.2432.9 ± 3.4 (26.7–38.1)34.0 ± 3.7 (28.8–39.6)
Body diam.18.2518.1 ± 2.5 (14.1–24.3)15.6 ± 1.8 (12.7–19.4)
Nerve ring from anterior72.773.6 ± 5.6 (62.9–85.0)69.8 ± 3.1 (65.5–75.7)
Excretory pore form anterior83.6794.3 ± 5.0 (83.7–108.2)88.9 ± 6.0 (71.9–98.7)
Esophagus length109.17113.6 ± 8.3 (91.0–127.6)114.4 ± 4.1 (107.0–119.3)
Vulva to anus distance (VA)80.579.1 ± 7.5 (66.7–90.3)-
Post vulval uterine sac40.7636.5 ± 7.4 (21.8–51.6)-
PUS/VA (%)50.6346.0 ± 7.6 (32.7–69.4)-
Ovary or testis length266.18254.6 ± 22.6 (217.7–303.6)238.8 ± 24.1 (190.3–287.8)
Anal (cloacal) body diam.13.0512.0 ± 2.0 (9.6–16.8)10.8 ± 1.2 (9.0–12.9)
Tail length68.9265.5 ± 5.4 (58.2–76.9)60.8 ± 4.3 (49.9–68.0)
Spicule length (arc line)--15.4 ± 0.6 (14.7–16.7)
Gubernaculum length--5.3 ± 0.4 (4.4–6.0)
DOI: https://doi.org/10.2478/jofnem-2025-0003 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Nov 4, 2024
Published on: Feb 27, 2025
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2025 Che-Chang Liang, Pei-Chen Chen, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.