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Exploring the use of metabarcoding to reveal eukaryotic associations with mononchids nematodes

Journal of Nematology
Volume 56 (2024): Issue 1 (March 2024)
By:
Open Access
|May 2024

Figures & Tables

Figure 1:

Detection of amplification fragments of rDNA 18S from 10 Mononchid species. A: Amplification with primer pair TAReuk454FWD1/TAReukREV3, lines 1–4 are M. brachyuris, C. parvus, Miconchus sp., and Prionchulus sp. respectively; B: Amplification with primer pair EUK20f/EUK302r+3, lines 1–6 are M. brevicaudatus, M. hawaiiensis, M. sigmaturellus, M. vulvapapillatus, Mylonchulus sp. and Anatonchus sp. respectively. M: DNA marker DL2000.
Detection of amplification fragments of rDNA 18S from 10 Mononchid species. A: Amplification with primer pair TAReuk454FWD1/TAReukREV3, lines 1–4 are M. brachyuris, C. parvus, Miconchus sp., and Prionchulus sp. respectively; B: Amplification with primer pair EUK20f/EUK302r+3, lines 1–6 are M. brevicaudatus, M. hawaiiensis, M. sigmaturellus, M. vulvapapillatus, Mylonchulus sp. and Anatonchus sp. respectively. M: DNA marker DL2000.

Figure 2:

Diversity of eukaryotic organisms presented in 10 mononchid species at phylum level. The classification system is based on the eukaryote classification system of Adl et al., (2012).
Diversity of eukaryotic organisms presented in 10 mononchid species at phylum level. The classification system is based on the eukaryote classification system of Adl et al., (2012).

Figure 3:

Diversity of eukaryotic organisms presented in 10 mononchid species at order level. The classification system is based on the eukaryote classification system of Adl et al., (2012).
Diversity of eukaryotic organisms presented in 10 mononchid species at order level. The classification system is based on the eukaryote classification system of Adl et al., (2012).

Figure S1:

Relative abundance of eukaryotic organisms presented in 10 mononchid species at different taxon levels. The percentages of each of the phyla or orders are calculated over the total number of reads excluding the nematode reads.
Relative abundance of eukaryotic organisms presented in 10 mononchid species at different taxon levels. The percentages of each of the phyla or orders are calculated over the total number of reads excluding the nematode reads.

Sampling information of Mononchid populations used in this study_

SpeciesHabitatSampling locality
M. brachyurisGrass land soilLinzhi, Tibetan
C. parvusMoss soilZhouzhi, Shaanxi
Miconchus sp.Moss soilNanjing, Jiangsu
Prionchulus sp.Populus sp. rhizosphereQiqihar, Heilongjiang
M. brevicaudatusMoss soilZhouzhi, Shaanxi
M. hawaiiensisMoss soilNanjing, Jiangsu
M. sigmaturellusDigitaria sp. rhizosphereNanyang, Henan
M. vulvapapillatusMoss soilHulun Buir, Inner Mongolia
Mylonchulus sp.Populus sp. rhizosphereQiqihar, Heilongjiang
Anatonchus sp.Moss soilZhouzhi, Shaanxi

Primer pair used, generated reads, and ASVs per mononchid sample_

SpeciesReadsASVsPrimer used
KingdomPhylumOrder
M. brachyuris4343432TAR
C. parvus4242363TAR
Miconchus sp.2317231523156TAR
Prionchulus sp.2142142113TAR
M. brevicaudatus5983598336187EUK
M. hawaiiensis43008426724061734EUK
M. sigmaturellus31737304222878189EUK
M. vulvapapillatus39443944392913EUK
Mylonchulus sp.34869348633486415EUK
Anatonchus sp.35140351403495930EUK
DOI: https://doi.org/10.2478/jofnem-2024-0016 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Nov 18, 2023
Published on: May 10, 2024
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
18S,
dietary,
ecological,
metabarcoding,
method,
sequencing,
universal
Related subjects:
Life sciences,
Life sciences, other

© 2024 Joseph O. Maosa, Siqi Wang, Shuhan Liu, Hongmei Li, Xue Qing, Wim Bert, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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