Nematicidal Properties of Chitosan Nanoformulation

R. Mouniga; B. Anita; A. Lakshmanan; A. Shanthi; G. Karthikeyan

doi:10.2478/jofnem-2023-0033

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Nematicidal Properties of Chitosan Nanoformulation

Journal of Nematology

Volume 55 (2023): Issue 1 (February 2023)

By: R. Mouniga, B. Anita, A. Lakshmanan, A. Shanthi and G. Karthikeyan

Open Access

|Aug 2023

Correction

This article was corrected on Jun 11, 2025

Figures & Tables

(A) Particle size of chitosan nanospheres. (B) Zeta potential of chitosan nanospheres.

Transmission Electron Microscope (TEM) micrograph of chitosan nanospheres.

FT-IR spectroscopy of 1% chitosan nanospheres formulation.

Scanning Electron Microscope images of chitosan nanospheres treated eggs (left side) and chitosan nanospheres untreated eggs (right side).

Table 1.

Effect of chitosan nanospheres on the egg hatching of M. incognita

Treatments	Number of hatched juveniles (Mean of four replications)
Treatments	24 h	48 h	72 h
Mean and transformed value	Mean and transformed value	Mean and transformed value
Chitosan nanospheres at 100 ppm	12.50^b (3.31)	16.50^b (4.84)	41.50^b (6.29)
Chitosan nanospheres at 500 ppm	10.50^b (3.09)	14.16^b (3.61)	26.50^b (4.79)
Chitosan nanospheres at 1000 ppm	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)
Chitosan nanospheres at 5000 ppm	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)
Control- tap water	38.83^c (6.21)	61.66^c (7.85)	84.80^c (17.27)
SEd	0.72	0.46	1.12
CD (p=0.01%)	2.29	1.48	3.88

* Figures in parentheses are square root transformed value. In a column, means followed by common different from each other at 1% level by DMRT.

Table 2.

Effect of chitosan nanospheres on M. incognita juvenile mortality

Treatments	Juvenile mortality in percentage (Mean of four replications)
Treatments	24 h	48 h	72 h
Chitosan nanospheres at 100 ppm	13.00^b (5.40)	21.5^c (3.47)	26.5^c (5.10)
Chitosan nanospheres at 500 ppm	27.25 ^b (5.14)	31.25^b (5.67)	28.25^c (5.26)
Chitosan nanospheres at 1000 ppm	29.00 ^b (5.85)	36.00^b (5.96)	46.25^b (6.70)
Chitosan nanospheres at 5000 ppm	100.00^a (10.02)	10.00^a (10.02)	100.00^a (10.02)
Control	0.00^c (0.707)	0.00^d (0.70)	0.00^d (0.70)
SEd	1.52	0.72	0.55
CD (p=0.01%)	4.48	2.14	1.64

* Figures in parentheses are square root transformed value. In a column, means followed by common alphabet are significantly different from each other at 1% level by DMRT.

Efficacy of different doses of chitosan nanospheres (1%) on *M. incognita* under pot culture conditions.

Table 3.

Effect chitosan nanospheres against root knot nematode, M. incognita under pot culture conditions

Treatments	Mean of three replications
	Number of galls/5g of roots	Number of egg masses/5g of roots	Number of females/5g of roots	Number of J2/100 cc of soil
	Mean and transformed value	Mean and transformed value	Mean and transformed value	Mean and transformed value
Chitosan nanospheres 1ml/plant+ M. incognita	13.30^a (3.51)	11.67^bcd (3.32)	17.67^cd (4.25)	48.60^bc (12.06)
Chitosan nanospheres 2ml/plant+ M. incognita	10.00^a (3.12)	6.00^b (2.53)	14.60^b (3.87)	28.30^ab (5.36)
Chitosan nanospheres 3ml/plant+ M. incognita	15.00^a (3.87)	7.33^bc (2.73)	17.60^cd (4.25)	40.30^bc (6.38)
Chitosan 1ml/plant+ M. incognita	18.00^a (4.28)	19.00^c (4.41)	22.30^f (4.76)	62.00^bcd (7.90)
Chitosan 2ml/plant + M. incognita	17.00^a (4.25)	10.00^bcd (3.22)	16.67^c (4.13)	46.70^bc (6.86)
Chitosan 3ml/plant + M. incognita	17.60^a (3.34)	10.66^bcd (3.30)	17.90^d (4.19)	44.70^bc (6.66)
Velum prime 0.5ml/plant + M. incognita	25.67^a (4.87)	15.67^de (3.95)	19.30^e (4.44)	32.67^bc (5.75)
P. lilacinum 1g/plant+M. incognita	17.67^a (4.25)	13.39^cde (1.37)	18.67^e (4.44)	50.60^bc (7.14)
Chitosan nanospheres without nematode Inoculums	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)
Untreated control	61.30^c (7.83)	37.16^f (6.04)	43.67^g (6.63)	105.60^cd (10.21)
SEd	0.96	0.47	0.06	2.26
CD (p=0.01%)	2.73	1.36	0.18	6.44

* Figures in parentheses are square root transformed value. In a column, means followed by common alphabet are significantly different from each other at 1% level by DMRT.

Table 4.

Effect of chitosan nanospheres against root knot nematode, M. incognita under field conditions

Treatments	Mean of three replications
	Number of galls/5g of roots¹	Number of egg masses/5g of roots¹	Number of females/5g of roots¹	Number of J2/100 cc of soil¹	Fruit weight (kg)/plot²	Fruit weight (tonne)/ha²
	Mean and transformed value	Mean and transformed value	Mean and transformed value	Mean and transformed value	Mean and transformed value	Fruit weight (tonne)/ha²
Chitosan nanospheres (1%)-5ml/plant	5.61^ab (2.29)	3.34^b (1.82)	18.31^b (4.26)	50.63^c (7.11)	95^a (18.75)	14.25
Chitosan nanospheres (2%)-5ml/plant	3.31^a (1.81)	0.99^ab (1.14)	17.38^b (4.16)	35.00^b (5.90)	89.5^b (11.87)	13.42
Chitosan (1%) - 5ml/plant	11.25^b (3.10)	3.62^b (1.93)	20.90^b (4.54)	58.54^e (7.61)	86^bc (7.5)	12.90
Chitosan (2%)-5ml/plant	4.91^ab (2.30)	1.88^ab (1.52)	17.79^b (4.19)	48.75^b (6.92)	83^cd (3.75)	12.45
Velum prime 500ml/acre	2.83^a (1.60)	0.09^a (0.75)	11.42^a (3.35)	30.45^a (5.47)	99^a (23.75)	14.85
Carbofuran 3G- 1kg ai/ha	7.32^b (2.95)	1.43^ab (1.35)	23.20^b (4.78)	54.23^d (7.34)	89^b (11.25)	13.35
Untreated control	44.00^c (0.62)	22.3^c (4.77)	57.25^c (7.57)	165.80^f (12.73)	80^d	12.00
SEd	0.46	0.37	0.37	0.10	2.28
CD (p=0.05%)	1.00	0.80	0.80	0.22	4.90

* ¹ and ²-Figures in parentheses are square root transformed value and increased over control respectively. In a column, means followed by common alphabet are significantly different from each other at 1% level by DMRT.

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DOI: https://doi.org/10.2478/jofnem-2023-0033 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Submitted on: Feb 1, 2023

Published on: Aug 24, 2023

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

Chitosan nanospheres,

Meloidogyne incognita,

tomato,

Related subjects:

© 2023 R. Mouniga, B. Anita, A. Lakshmanan, A. Shanthi, G. Karthikeyan, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 55 (2023): Issue 1 (February 2023)

Nematicidal Properties of Chitosan Nanoformulation

Correction

Figures & Tables

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Table 1.

Table 2.

Figure 5:

Table 3.

Table 4.

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