Nematicidal Properties of Chitosan Nanoformulation

Mouniga, R.; Anita, B.; Lakshmanan, A.; Shanthi, A.; Karthikeyan, G.

Nematicidal Properties of Chitosan Nanoformulation

Journal of Nematology

Volume 55 (2023): Issue 1 (February 2023)

By:

R. Mouniga, B. Anita, A. Lakshmanan, A. Shanthi and G. Karthikeyan

Open Access

|Aug 2023

Figures & Tables

(A) Particle size of chitosan nanospheres. (B) Zeta potential of chitosan nanospheres.

Transmission Electron Microscope (TEM) micrograph of chitosan nanospheres.

FT-IR spectroscopy of 1% chitosan nanospheres formulation.

Scanning Electron Microscope images of chitosan nanospheres treated eggs (left side) and chitosan nanospheres untreated eggs (right side).

Efficacy of different doses of chitosan nanospheres (1%) on M. incognita under pot culture conditions.

Effect of chitosan nanospheres against root knot nematode, M_ incognita under field conditions

Treatments	Mean of three replications

	Number of galls/5g of roots¹	Number of egg masses/5g of roots¹	Number of females/5g of roots¹	Number of J2/100 cc of soil¹	Fruit weight (kg)/plot²	Fruit weight (tonne)/ha²

	Mean and transformed value	Mean and transformed value	Mean and transformed value	Mean and transformed value	Mean and transformed value
Chitosan nanospheres (1%)-5ml/plant	5.61^ab (2.29)	3.34^b (1.82)	18.31^b (4.26)	50.63^c (7.11)	95^a (18.75)	14.25
Chitosan nanospheres (2%)-5ml/plant	3.31^a (1.81)	0.99^ab (1.14)	17.38^b (4.16)	35.00^b (5.90)	89.5^b (11.87)	13.42
Chitosan (1%) - 5ml/plant	11.25^b (3.10)	3.62^b (1.93)	20.90^b (4.54)	58.54^e (7.61)	86^bc (7.5)	12.90
Chitosan (2%)-5ml/plant	4.91^ab (2.30)	1.88^ab (1.52)	17.79^b (4.19)	48.75^b (6.92)	83^cd (3.75)	12.45
Velum prime 500ml/acre	2.83^a (1.60)	0.09^a (0.75)	11.42^a (3.35)	30.45^a (5.47)	99^a (23.75)	14.85
Carbofuran 3G- 1kg ai/ha	7.32^b (2.95)	1.43^ab (1.35)	23.20^b (4.78)	54.23^d (7.34)	89^b (11.25)	13.35
Untreated control	44.00^c (0.62)	22.3^c (4.77)	57.25^c (7.57)	165.80^f (12.73)	80^d	12.00
SEd	0.46	0.37	0.37	0.10	2.28
CD (p=0.05%)	1.00	0.80	0.80	0.22	4.90

Effect of chitosan nanospheres on M_ incognita juvenile mortality

Treatments	Juvenile mortality in percentage (Mean of four replications)

	24 h	48 h	72 h
Chitosan nanospheres at 100 ppm	13.00^b (5.40)	21.5^c (3.47)	26.5^c (5.10)
Chitosan nanospheres at 500 ppm	27.25 ^b (5.14)	31.25^b (5.67)	28.25^c (5.26)
Chitosan nanospheres at 1000 ppm	29.00 ^b (5.85)	36.00^b (5.96)	46.25^b (6.70)
Chitosan nanospheres at 5000 ppm	100.00^a (10.02)	10.00^a (10.02)	100.00^a (10.02)
Control	0.00^c (0.707)	0.00^d (0.70)	0.00^d (0.70)
SEd	1.52	0.72	0.55
CD (p=0.01%)	4.48	2.14	1.64

Effect chitosan nanospheres against root knot nematode, M_ incognita under pot culture conditions

Treatments	Mean of three replications

	Number of galls/5g of roots	Number of egg masses/5g of roots	Number of females/5g of roots	Number of J2/100 cc of soil

	Mean and transformed value	Mean and transformed value	Mean and transformed value	Mean and transformed value
Chitosan nanospheres 1ml/plant+ M. incognita	13.30^a (3.51)	11.67^bcd (3.32)	17.67^cd (4.25)	48.60^bc (12.06)
Chitosan nanospheres 2ml/plant+ M. incognita	10.00^a (3.12)	6.00^b (2.53)	14.60^b (3.87)	28.30^ab (5.36)
Chitosan nanospheres 3ml/plant+ M. incognita	15.00^a (3.87)	7.33^bc (2.73)	17.60^cd (4.25)	40.30^bc (6.38)
Chitosan 1ml/plant+ M. incognita	18.00^a (4.28)	19.00^c (4.41)	22.30^f (4.76)	62.00^bcd (7.90)
Chitosan 2ml/plant + M. incognita	17.00^a (4.25)	10.00^bcd (3.22)	16.67^c (4.13)	46.70^bc (6.86)
Chitosan 3ml/plant + M. incognita	17.60^a (3.34)	10.66^bcd (3.30)	17.90^d (4.19)	44.70^bc (6.66)
Velum prime 0.5ml/plant + M. incognita	25.67^a (4.87)	15.67^de (3.95)	19.30^e (4.44)	32.67^bc (5.75)
P. lilacinum 1g/plant+M. incognita	17.67^a (4.25)	13.39^cde (1.37)	18.67^e (4.44)	50.60^bc (7.14)
Chitosan nanospheres without nematode Inoculums	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)
Untreated control	61.30^c (7.83)	37.16^f (6.04)	43.67^g (6.63)	105.60^cd (10.21)
SEd	0.96	0.47	0.06	2.26
CD (p=0.01%)	2.73	1.36	0.18	6.44

Effect of chitosan nanospheres on the egg hatching of M_ incognita

Treatments	Number of hatched juveniles (Mean of four replications)

	24 h	48 h	72 h
	Mean and transformed value	Mean and transformed value	Mean and transformed value
Chitosan nanospheres at 100 ppm	12.50^b (3.31)	16.50^b (4.84)	41.50^b (6.29)
Chitosan nanospheres at 500 ppm	10.50^b (3.09)	14.16^b (3.61)	26.50^b (4.79)
Chitosan nanospheres at 1000 ppm	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)
Chitosan nanospheres at 5000 ppm	0.00^a (0.70)	0.00^a (0.70)	0.00^a (0.70)
Control- tap water	38.83^c (6.21)	61.66^c (7.85)	84.80^c (17.27)
SEd	0.72	0.46	1.12
CD (p=0.01%)	2.29	1.48	3.88

DOI: https://doi.org/10.2478/jofnem-2023-0033 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Submitted on: Feb 1, 2023

Published on: Aug 24, 2023

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

Chitosan nanospheres,

tomato,

Related subjects:

© 2023 R. Mouniga, B. Anita, A. Lakshmanan, A. Shanthi, G. Karthikeyan, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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Nematicidal Properties of Chitosan Nanoformulation

Figures & Tables

Figure 1:

Figure 2:

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Figure 4:

Figure 5:

Effect of chitosan nanospheres against root knot nematode, M_ incognita under field conditions

Effect of chitosan nanospheres on M_ incognita juvenile mortality

Effect chitosan nanospheres against root knot nematode, M_ incognita under pot culture conditions

Effect of chitosan nanospheres on the egg hatching of M_ incognita

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