Plant-Parasitic Nematodes Associated with Cannabis sativa in Florida

*Field site*	*County*	*Elevation*	*Soil type*	*Coordinates*
GCREC^a – Central FL	Hillsborough	31 m	Myakka fine sand	27.76° N, 82.23° W
TREC^b – South FL	Miami—Dade	1 m	Shallow Krome gravelly loam	25.47° N, 80.50° W
NFREC^c – North FL	Gadsden	63 m	Orangeburg loamy fine sand	30.58° N, 84.59° W

a GCREC, Gulf Coast Research and Education Center, Wimauma, FL.

b TREC, Tropical Research and Education Center, Homestead, FL.

c NFREC = North Florida Research and Education Center, Quincy, FL.

Table 2.

Hemp cultivars with their specific use, country of origin and year(s) planted at each location.

*Cultivars*	*Type/Use*	*Origin*	*Year Planted*
GCREC^a,b and TREC^c
Yuma-2	Fiber/Dual	China	2019
Bama	Fiber/Dual	China	2019
Puma-3	Fiber/Dual	China	2019, 2020
Tygra	Fiber/Dual	Poland	2019
Carmagnola Selezionata	Fiber/Dual	Italy	2019
Eletta Campana	Fiber/Dual	Italy	2019
Cherry Blossom x T1	CBD	US	2019
Cherry Blossom	CBD	US	2019
*TREC*
Berry Blossom	CBD	US	2019
Canda	Fiber/Dual	Canada	2019
Si-1	Fiber/Dual	China	2019
Fibranova	Fiber/Dual	Italy	2019
Han-NE	Fiber/Dual	China	2019, 2020
Carmagnola	Fiber/Dual	Italy	2020
NBS	CBD	US	2020
Wife	CBD	US	2020
Maverick	CBD	US	2020
*NFREC^d*
KG 9201	CBD	US	2019
KG 9202	CBD	US	2019
Cherry Wine	CBD	US	2019, 2020
Cherry Blossom x T1	CBD	US	2019
Cherry Blossom	CBD	US	2019, 2020
Berry Blossom	CBD	US	2020
Queens Dream	CBD	US	2020
Cinderella Story	CBD	US	2020
Cloud Berry	CBD	US	2020
Cherry Blonde	CBD	US	2020
Hot Blonde	CBD	US	2020

a GCREC, Gulf Coast Research and Education Center.

b Planting at GCREC was done in fall 2019 and spring 2020, and at TREC and NREC in summer 2019 and summer 2020.

c TREC, Tropical Research and Education Center.

d NFREC, North Florida Research and Education Center.

Table 3.

Nematode soil populations (no./200 cc soil) at harvest of eight different hemp cultivars for two consecutive seasons, GCREC, Fall 2019 (trial 1) and Spring 2020 (trial 2)^a.

*Sept. – Dec. 2019 (Fall)*	*Bacterivore*	*Fungivore*	*Omnivore*	*Stubby root*	*Root-knot*	*Sting*
Yuma-2	49	31	12	31 a	0	0
Bama	117	18	7	21 ab	0	0
Puma-3	120	32	12	32 a	0	1
Tygra	70	30	7	6 b	0	0
Carmagnola Selezionata	121	30	9	12 ab	0	1
Eletta Campana	89	19	7	11 ab	0	0
Cherry Blossom x T1	121	32	5	23 ab	0	1
Cherry Blossom	72	25	7	15 ab	0	1
P value	0.24	0.75	0.31	0.02	-	-

*Feb.-May 2020 (Spring)*	*Bacterivore*	*Fungivore*	*Omnivore*	*Stubby root*	*Root-knot*	*Sting*
Yuma-2	-	-	-	18 abc	16	15
Bama	-	-	-	62 ab	437	3
Puma-3	-	-	-	62 ab	610	13
Tygra	-	-	-	11 bc	289	14
Carmagnola Selezionata	-	-	-	11 bc	9	16
Eletta Campana	-	-	-	8 c	117	9
Cherry Blossom x T1	-	-	-	65 a	100	8
Cherry Blossom	-	-	-	24 abc	315	32
P value	-	-	-	0.002	0.16	0.17

a Numbers listed describe the number of a type of nematode found in 200 cc of soil collected from the root area of the hemp cultivars listed. There are multiple types/uses of hemp here. P values were calculated on Log (x+1) transformed data according to Tukey’s HSD where P ≤ 0.05; bacterivores, fungivores, omnivores were not counted in the second season.

Table 4.

Nematode soil populations (no./200 cc soil) at harvest of different hemp cultivars for two consecutive seasons, summer 2019 and summer 2020; TREC soil survey results^a.

*Summer 2019*	*Bacterivore*	*Fungivore*	*Omnivore*	*Reniform*	*Stunt*	*Spiral*	*Ring*	*RKN*
Puma-3	116	42	0	5,564 a	112	176	12	0
Bama	70	74	0	1,987 ab	156	221	26	0
Berry Blossom	28	52	0	574 abc	32	92	2	0
Tygra	22	26	2	762 abc	8	26	14	0
Carmagnola Selezionata	52	100	8	4,872 a	80	84	12	0
Helena	68	48	2	648 abc	24	48	0	0
Canda	80	116	8	480 abc	40	54	6	0
Si-1	54	21	0	319 c	178	286	0	0
Fibranova	83	27	0	333 bc	15	373	0	0
Yuma-2	39	29	2	636 abc	119	524	1	0
Han NE	25	12	1	195 c	11	266	0	0
Cherry Blossom x T1	42	25	0	989 abc	38	119	0	0
Eletta Campana	36	52	1	844 abc	30	216	14	0
P value	0.06	0.04	0.05	0.004	0.20	0.19	0.19	-

*Summer 2020*	*Bacterivore*	*Fungivore*	*Omnivore*	*Reniform*	*Stunt*	*Spiral*	*Ring*	*RKN*
Puma-3	141	31	1	78	79	148	73	41
Carmagnola	173	43	1	101	81	109	65	74
Han NE	180	42	4	214	216	409	17	62
NBS	73	19	2	22	54	256	59	20
Wife	215	50	2	69	108	686	447	57
Maverick	111	32	1	32	53	289	35	18
P value	0.21	0.22	0.82	0.15	0.11	0.06	0.25	0.57

Table 5.

Nematode soil populations (no./200 cc soil) at harvest of different hemp cultivars for two consecutive seasons, summer 2019 and summer 2020; NFREC soil survey results^a.

*Summer 2019*	*Bacterivore*	*Fungivore*	*Omnivore*	*Reniform*	*Stunt*	*Spiral*	*Ring*	*RKN^b*
KG 9201	139	40	0	41	1	0	0	3
Cherry Wine	158	47	0	92	4	2	0	5
KG 9202	312	74	1	127	3	0	1	4
Cherry Blossom x T1	307	80	0	239	1	6	0	65
Cherry Blossom	304	68	1	191	2	3	0	16
P value	0.42	0.30	0.54	0.43	0.71	0.36	0.44	0.33

*Summer 2020*	*Bacterivore*	*Fungivore*	*Omnivore*	*Reniform*	*Stunt*	*Spiral*	*Ring*	*RKN*
Berry Blossom	309	52	0	67	0	2	0	9
Queens Dream	212	32	1	40	2	0	0	3
Cinderella Story	119	61	0	103	1	2	0	38
Cherry Blossom	441	86	0	328	2	4	0	4
Cloud Berry	320	75	2	101	3	0	2	33
Cherry Blonde	167	50	0	53	1	1	0	41
Hot Blonde	319	115	0	453	1	9	0	28
Cherry Wine	224	64	1	90	2	1	0	83
P value	0.95	0.96	0.19	0.83	0.76	0.34	0.62	0.17

a Numbers listed describe the number of a type of nematode found in 200 cc of soil collected from the root area of the hemp cultivars listed. There are only CBD hemp cultivars here. P values were calculated on Log (x+1) transformed data according to Tukey’s HSD where P ≤ 0.05.

b RKN, root-knot.

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DOI: https://doi.org/10.2478/jofnem-2023-0018 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Submitted on: Jul 29, 2022

Published on: Jul 6, 2023

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

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© 2023 J. Desaeger, J. Coburn, J. Freeman, Z. Brym, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 55 (2023): Issue 1 (February 2023)